Conference on

“Promoting the Health of an Aging Population”

at the Year 2004 Annual Meeting of the Gerontological Society of America

 

Symposium Topic

Chair/Co-Chair

The Paradox of the Insulin Signaling Pathway and Longevity

Nir Barzilai

Profiles of Healthy Aging: Quantifying Biological Age and Function

David Welsh

Mitochondria and Aging: Cause or Effect?

David Nicholls

Pathobiology and Degenerative Disorders of Aging – A Model Systems Approach

Monica Driscoll

Nature, Nurture, and Longevity: What Most of the World’s Organisms Can Tell Us

Stephen Helfand

Whole Animal Energy Metabolism in Lab and Field: Is There a Link to Aging?

Roger McCarter

The Cellular and Molecular Mechanisms of Immunosenescence

John Mountz

Recent Breakthroughs in Understanding Progeria and Premature Aging Syndromes

Ted Brown

Chance Events in Aging: Findings from Twin Studies

Nicholas Greco/Michal Jazwinski

 

(1)  The Paradox of the Insulin Signaling Pathway and Longevity

 

Chair:  Nir Barzilai, Institute for Aging Research, Diabetes Center, Departments of Medicine and Molecular Genetics, Albert Einstein College of Medicine, Bronx NY 10461

 

      Down regulation of the insulin signaling pathway is associated with significant longevity in lower species. However, similar alterations in insulin signaling and insulin action are associated with variety of age-related diseases leading to death in mammals. This symposium brings together experts in insulin action to discuss this paradox. Dr. Rossetti will discuss the catabolic and variable metabolic effects caused by activation of insulin signaling in the brain, while opposite effects are noted by the activation of the peripheral insulin signaling pathway. Because nematodes have only a central insulin signaling pathway, this may explain part of the paradox. Dr. Barzilai, will expand on the divergence between IGF and insulin signaling pathways in mammalian and the role of low IGF states in longevity, further adding to the explanation of the paradox. Dr. Barzilai will also demonstrate the harmful effects of decreased insulin action on mammalian models. Finally, since intact insulin action is required for mammalian longevity, Dr. Kenyon will summarize the efforts to identify the relevant downstream pathways that may be common with lower species. Such possibilities may include activation of small heat-shock proteins, upregulation of a wide variety of genes, including cellular stress-response, antimicrobial and metabolic genes, and downregulation of specific life-shortening genes with potential impact on the elderly population.

 

L. Rossetti (Diabetes Center, Albert Einstein College of Medicine, Bronx, NY) Insulin signaling pathway in brain and in the periphery have opposite physiologic effects in mammalians

 

N. Barzilai (Institute for Aging Research, Albert Einstein College of Medicine, Bronx, NY) Insulin resistance as a risk for a shorter life span in mammalians

 

C. Kenyon (Department of Biochemistry and Biophysics, University of California, San Francisco, CA) How may longevity be induced by alterations in the insulin signaling pathway in nematodes

 

Open slot: Late-breaking developments

 

(2) Profiles of Healthy Aging: Quantifying Biological Age and Function

 

Chair: David Welsh, Department of Medicine, Louisiana State University Health Sciences Center, New Orleans, LA

 

      While a person’s lifespan can be relatively easily measured, it has become clear that chronological age is not equivalent to physiologic age.  Further, biomarkers that have been shown to be predictive of future outcomes may not necessarily reflect current functionality.  Much work has been completed in recent years attempting to define the concepts of physiologic (biologic) age, successful aging, and frailty.  Despite this effort there is no universally accepted definition or “gold standard” of successful aging, nor is there a definitive measurement instrument.  This symposium will facilitate discussion and thought on current and future methodologies by which to quantify healthy or successful aging.  Featured speakers will propose definitions of successful (and unsuccessful) aging, review the data from investigations that have validated indices or profiles of frailty and provide insights on future directions to be pursued in validating quantitative measurement instruments reflective of successful aging.

Kenneth Rockwood (Division of Geriatric Medicine, Dalhousie University, Halifax, NS, Canada) The frailty index and biologic age


Linda P. Fried (The Johns Hopkins University School of Medicine, The Johns Hopkins University School of Hygiene and Public Health, Baltimore, MD) The phenotype of frailty

 

James F. Fries* (Department of Medicine, Stanford University School of Medicine, Palo Alto, CA) Measuring and monitoring success in compressing morbidity

 

Open slot: Late-breaking developments

(3) Mitochondria and Aging: Cause or Effect?

 

Chair:   David Nicholls, Buck Institute for Age Research, Novato CA

 

      There is considerable evidence that mitochondrial bioenergetic function is impaired in aging. A fundamental question, however, is whether mitochondrial dysfunction is one of the causes of aging, or whether mitochondrial dysfunction is merely a secondary consequence of the aging process. David Nicholls will review current hypotheses from the standpoint of a basic bioenergeticist, Christiaan Leeuwenburgh will discuss current findings relating caloric restriction to mitochondrial function and aging and Michael Breitenbach will cover insights into mitochondria and aging obtained from yeast studies.

David Nicholls (Buck Institute for Age Research, Novato CA) Mitochondria and aging: a review of current hypotheses

Christiaan Leeuwenburgh (Biochemistry of Aging Laboratory, College of Health and Human Performance, College of Medicine, Center for Exercise Science, University of Florida Gainesville, FL) Aging, caloric restriction, and mitochondrial energy production

Michael Breitenbach (Institute of Genetics and General Biology, University of Salzburg, Salzburg, Austria) Yeast aging and mitochondrial function

Open slot: Late-breaking developments

(4)  Pathobiology and Degenerative Disorders of Aging – A Model Systems Approach

 

Chair:   Monica Driscoll, Department of Molecular Biology and Biochemistry, Rutgers, The State University of New Jersey, Piscataway, NJ

 

      One of the most promising themes to emerge from gerontological research in recent years is that several processes contributing to age-related decline involve molecular mechanisms conserved from lower organisms to humans.  Moreover, modeling of specific disease states in experimental organisms has established powerful paradigms for age-associated degenerative diseases (such as Alzheimer's disease and Parkinson's disease) that are providing novel insights into disease progression and prevention.  This symposium will highlight advances into our understanding of fundamental processes that contribute to age-related decline and underlie late-onset neurodegenerative conditions as gleaned from a model systems perspective.

 

Nancy M. Bonini (Department of Biology and HHMI, University of Pennsylvania, Philadelphia, PA) Pathological mechanisms for protein aggregation disorders in Drosophila

 

Frank LaFerla (Department of Neurobiology and Behavior, University of California, Irvine, CA) Intracellular Ab and synaptic dysfunction modeled in mouse

 

Monica Driscoll (Department of Molecular Biology and Biochemistry, Rutgers University, Piscataway, NJ) The genetic control of glycation, sarcopenia, and healthspan in C. elegans

 

Open slot: Late-breaking developments

 

(5) Nature, Nurture, and Longevity: What Most of the World’s Organisms Can Tell Us

 

Chair: Stephen Helfand, Department of Genetics and Developmental Biology, University of Connecticut Health Center, Farmington, CN

 

      One of the most interesting discoveries in recent years is the realization that many of the biochemical and physiological pathways involved in the determination of life span appear to be shared between organisms as diverse as mammals and yeast.  Thus model organisms may be able to tell us more about the process of aging in humans than we could have imagined.  One of the many advantages of model organisms is the ability to readily and rapidly manipulate their genetic constituency and epigenetic environment.  This allows experimenters to quickly test physiological systems thought to be important in human longevity and to develop molecular and pharmaceutical interventions capable of extending life span.  In this symposium we will examine the power of three important model systems: yeast, nematodes, and fruit flies to help us understand the basic biology of the aging process and identify interventions, both pharmaceutical and environmental, that may contribute to our understanding of human aging and the potential amelioration of age-associated diseases.

 

David Sinclair (Department of Pathology, Harvard Medical School, Boston, MA) Small molecule and genetic mimetics of calorie restriction

 

Gordon Lithgow (Buck Institute for Age Research, Novato CA) Lifespan determination by stress response in C. elegans
 
Stephen L. Helfand (Department of Genetics and Developmental Biology, University of Connecticut Health Center, Farmington, CN) I'm not dead yet: a gene that doubles the life span of the fly

 

Open slot: Late-breaking developments

 

(6) Whole Animal Energy Metabolism in Lab and Field: Is There a Link to Aging?

 

Chair:  Roger McCarter, Department of Physiology, University of Texas Health Science Center at San Antonio and GRECC, Audie L. Murphy Memorial VA Hospital, San Antonio, TX

        

      There is widespread belief that energy metabolism, in particular oxidative energy metabolism involves deleterious consequences which accumulate over the lifespan and constitute important mechanisms of aging.  This view is consistent with theories of aging, from Rate of Living to Oxidative Free Radical and Mitochondrial theories of aging.  It is also consistent with the age-retarding effects of calorie restriction, in which it is known that the anti-aging effect is a consequence of reduced daily energy intake, rather than specific nutrient intake.  Mechanisms linking energy metabolism to aging processes are however controversial and have not been precisely identified.  The goal of this symposium is to critically evaluate the involvement of whole animal energy metabolism in aging across wide phylogenetic lines.  Dr. Van Voorhies will discuss possible links between metabolic rate and aging in invertebrates, with particular emphasis on provocative new findings in different lines of Drosophila melanogaster.  Dr. Kemnitz will evaluate the relevance of total versus resting energy expenditure in mechanistic effects of lifelong calorie restriction in non-human primates.  Dr. McCarter will examine evidence in calorie restricted laboratory rats and mice of a link between longevity and altered energy metabolism, for both sedentary and vigorously exercising animals.  Dr. Speakman  will discuss measurement of total daily energy expenditure of animals in the field and evaluate the possible relevance of metabolic rate to aging in non-laboratory settings.  These presentations will provide a comprehensive overview of evidence, at the level of the whole animal, of the potential importance of energy metabolism in mechanisms of aging.

 

Wayne Van Voorhies (Department of Molecular Biology, New Mexico State University, Los Cruces, NM) Metabolic rate and lifespan in Drosophila

Roger McCarter (Department of Physiology, University of Texas Health Science Center, San Antonio, TX) Metabolic rate over the lifespan of calorie restricted  laboratory rats and mice

Joseph Kemnitz (Primate Center, University of Wisconsin, Madison, WI) Energy metabolism and nutrition in aging non- human primates

John Speakman (Department of Zoology, University of Aberdeen, Aberdeen, Great Britain) Energy metabolism of animals in the field

 

(7) The Cellular and Molecular Mechanisms of Immunosenescence

 

Chair:     John Mountz, University of Alabama at Birmingham, Department of Medicine, Division of Clinical Immunology and Rheumatology, Birmingham, AL

 

      The hallmark of immune senescence in the peripheral lymphoid system is an age-related defect in the CD4+ T cell helper response as well as CD8+ cytotoxic T cell response.  The process of immune senescence parallels the biological process of aging as one of the primary models for cell senescence. With aging this replicative senescence is associated with telomere shortening and slowing and arrest of the cell cycle.  Dr. Effros will discuss human immune senescence with a focus on the CD8 cytotoxic T cell population.  The thymic output of CD8+ T cells decreases faster than CD4+ T cells, and peripheral expansion of CD8+ T cells is decreased and becomes oligoclonal with age.  Underlying this dramatic alteration in human CD8+ T cell population dynamics is a cell cycle arrest resulting in replicative senescence.  Furthermore, T cells that have undergone replicative senescence exhibit down modulation of CD28.  The key role of cytotoxic T cells in elderly humans is to combat viral infection.  To this end, most vaccines that are used in the elderly are designed to enhance the CD8 cytotoxic T cell response.  Dr. Donna Murasko will discuss the limitations and efficacy of influenza vaccine in the elderly.  This vaccine relies heavily on the production of Th1 T cells and CD8+ cytotoxic T cell response.  Finally, the CD4+ T cell response is critical to promote both B cell response and CD8 cytotoxic T cell response.  Dr. Goronzy will discuss mechanisms of decreased CD4+ T cell response in the elderly.  The efficiency of CD4+ T cell response is highly dependent on the CD28 signaling pathway.  Dr. Goronzy will discuss the molecular basis of modulators of CD28 gene transcription as a fundamental mechanism for the decrease in the T cell response with age.  Dr. Mountz will discuss his work indicating the critical role of both a robust proliferative response and an active apoptotic process in T cells, which is associated with longevity.

 

Rita Effros (Department of Pathology and Laboratory Medicine, David Geffen School of Medicine, University of California, Los Angeles, CA) T cell replicative senescence in CD28 expression

 

Donna Murasko (College of Arts and Sciences, Drexel University, Philadelphia, PA) Age-related decrease in cytotoxic T cells to influenza

 

Jorg Goronzy (Department of Medicine and Immunology, Mayo Clinic, Rochester, MN) CD4+ T cells signaling defects with aging, role of CD28 signaling

 

John Mountz (Center for Aging, University of Alabama at Birmingham, Birmingham, AL) Genetic analysis of T cell senescence in mice and man

 

(8) Recent Breakthroughs in Understanding Progeria and Premature Aging Syndromes

 

Chair:  W. Ted Brown, Department of Human Genetics, Institute for Basic Research in Developmental Disabilities, Staten Island, NY

 

      Progeria, the Hutchinson-Gilford progeria syndrome (HGPS) (from the Greek for premature aging), is a rare disease of childhood with striking features resembling premature aging.  Other "premature aging syndromes", such as Werner syndrome (progeria of the adult) and Down syndrome, have provided valuable information regarding the phenomenon of aging.  Important clues about aging may also result from understanding the pathogenic mechanisms involved in HGPS. Recently, it was discovered that de novo mutations in the gene LMNA encoding the lamin A/C proteins cause HGPS.  Mutations of lamin A have been found in six other laminopathic disorders (Emery-Dreyfuss muscular dystrophy, dilated cardiomyopathy, familial partial lipodystrophy, limb girdle muscular dystrophy, Charcot-Marie-Tooth II, and mandibuloacral dysplasia).  LMNA mutations also are found in several adult Werner syndrome progeric patients with atypical features that are negative for WRN mutations.  This symposium will present an overview of HGPS and the latest research on the disorder, as well as cover funding opportunities.

 

W. Ted Brown (Department of Human Genetics, Institute for Basic Research in Developmental Disabilities, Staten Island, NY) Overview of HGPS and comparison with other laminopathies and premature aging syndromes

 

Francis Collins (National Institute for Human Genome Research, Bethesda, MD) Lamin defects in Progeria -The HGPS gene defect and what it means

 

Leslie Gordon (Progeria Research Foundation, Peabody, MA) Prospects for therapy of HGPS and the role of the Progeria Research Foundation in supporting research

 

Open slot: Late-breaking developments

 

(9)  Chance Events in Aging: Findings from Twin Studies

 

 

Co-Chairs:  Nicholas Greco1 and S. Michal Jazwinski2, 1Department of Anatomy and Neurobiology, Virginia Commonwealth University, Medical College of Virginia, Richmond, VA, 2Louisiana State University Health Sciences Center, New Orleans, LA

 

      Many events that occur during aging have been tied to genetic make-up.  Twin research provides us with a unique opportunity to investigate events of aging that are both the result of genetics and chance events or non-genetic changes.  Non-genetic cellular variations as a result of development have been found to influence the outcomes of the physiological aging process.  Many chance events of aging occur as a result of environmental influence.  Through longitudinal twin studies, it has been found that both genetic and environmental influences have an impact on normative cognitive change and age of onset.

 

Caleb Finch (Department of Neurobiology and School of Gerontology, University of Southern California, Los Angeles, CA) Cellular variations during development influence outcomes of aging

 

Margaret Gatz (Department of Psychology and School of Gerontology, University of Southern California, Los Angeles, CA), Chandra Reynolds (University of California Riverside, Riverside, CA), Nancy Pederson (Department of Psychology, Virginia Commonwealth Unversity, Richmond, VA) Genetic and environmental influences on normative cognitive change

 

Gerald McClearn* (Center for Developmental and Health Genetics, Pennsylvania State University, State College, PA) Genetics of complex behaviors in aging