Application of the combined approaches of microbial genetics,
molecular biology and biochemistry to the study of fundamental
biological processes will be demonstrated. The research literature
will be used to describe the current state of knowledge of the
molecular mechanisms of prokaryotic gene regulation and the genetic
biochemistry of chromosome structure, and DNA replication, repair,
and recombination. Prerequisites: BIOL 302 and BIOL 303 or consent
of instructor. BIOL 437 is recommended.
Molecular Biology Laboratory
This two-semester course is designed to demonstrate the approaches
and methodologies of molecular biology used to study the organization,
expression, and regulation of genes. Emphasis is on acquiring facility
in the design of experiments, analysis of data, and troubleshooting,
as well as in developing a broad range of technical skills and the
ability to use modern laboratory equipment. The first part of the
course focuses on in vitro or recombinant DNA/cloning techniques
and emphasizes the isolation, manipulation, and molecular characterization
of DNA and RNA. The second part involves in vivo genetic analysis
in selected prokaryotic and eukaryotic organisms. It includes mutagenesis
and genetic mapping in E. coli as well as use of gene fusions to
assess gene expression in vivo. Eukaryotic cell culture and immunological
techniques are used to illustrate their utility in molecular biology.
Prerequisites: BIOL 624, CHEM 437 and 437L; CHEM 438 recommended
as concurrent. Enrollment priority will be given to AMB students.
Molecular Biology Laboratory
Continuation of BIOL635L (Spring)
A lecture and discussion course that considers the two major aspects
of animal development: 1) the means by which, starting with a fertilized
egg, progeny cells progressively differentiate from their precursors
and one another to produce the ultimate diversity of the multicellular
organism; and 2) the processes by which this increasingly complex
population of cells is synthesized into a single integrated organism.
Weekly student-led discussion of papers from the literature, a term
paper, and three exams. (Fall).
Designed to emphasize cellular, molecular, and biochemical aspects
of basic developmental questions, this course will introduce the
student to modern approaches to determination, differentiation,
and morphogenesis. Experimental design and analysis of data are
emphasized. Topics include: molecular and cellular aspects of gametogenesis,
fertilization, embryogenesis, and continuous development in the
adult; mechanisms of intra- and intercellular communication; developmental
model system using unicellular organisms will be considered. May
be repeated for credit. Prerequisites: BIOL 442 and/or consent of
the instructor. May be repeated for credit.
This course will examine some of the methods by which the reception
of signals from the environment leads to the changes in gene
and protein activity in responding cells which constitute a biological
response. Signal transduction in the context of developmental
biology and neurobiology will be the main areas of study. Six
to eight topics will be covered in detail. The design and interpretation
of scientific experiments will be emphasized through critical
reading, analysis and presentation of original articles from
the primary literature. The use of genetic, molecular and biochemical
techniques to address questions in the field of signal transduction
will be examined. Prerequisites: BIOL 302 and 303.
A study of nervous system function at the cellular and organismic
level. Topics include: mechanisms underlying electrical activity
in nerve cells and synapses, transduction and integration of sensory
information, action of certain drugs and neurotoxic agents, activity
in population of neurons, trophic and plastic properties of nerve
cells, and neural systems analysis. Prerequisite: BIOL 305 or consent
of the instructor.
Bases of Behavior
Studies of behavioral patterns and their physiological bases. The
course begins with an extensive review of the fundamentals of neurobiology
and basic principles of animal behavior, followed by neurophysiological
analyses of specific invertebrate behaviors such as: locomotion,
feeding, prey capture and predator evasion, and learning. Prerequisite:
BIOL 305 or consent of the instructor.
Following a brief review of some important principles and techniques
in molecular biology, this course will pursue in depth such topics
as the cloning and characterization of chloroplast, mitochondrial,
and nuclear genomes in plants; interactions of the nuclear and chloroplast
gene products; genetic engineering of the nitrogen fixation genes,
DNA plant viruses, and the Agrobacterium Ti plasmid. The course
content will reflect the status of research in this rapidly developing
area. Prerequisites: BIOL 302 and BIOL 303, or consent of the instructor.
of Marine and Estuarine Animals
A study of the physiological specializations demanded by marine/estuarine
environments, including the following topics: physiological mechanisms
for coping with stresses imposed by extremes of temperature, salinity,
aerial exposure, and low oxygen concentrations; sensory physiology
including visual, chemical, and mechanical modalities; exogenous
and endogenous rhythms related to tidal or diel cycles; and bioluminescence.
The course will include one or more trips to field laboratories.
Prerequisite: BIOL 305.
and Quantitative Biology
Mathematical, statistical, and computer techniques used in quantitative
analysis of biological phenomena. Topics will include the theoretical
bases for commonly used univariate tests as well as multivariate
techniques such as discriminant, canonical factor and cluster analyses.
Applications of methods will be discussed. Data sets will be assigned
for analysis. Prerequisites: BIOL 301 and BIOL 309 (also listed
as STAT 350).
and Community Ecology
Advanced topics in population and community ecology. An overview
of current ecological theory is combined with a critical examination
of field studies and experiments from the ecological literature.
Major topics include population regulation and biological control
of pest species, evolution of life history patterns, interplay between
competition and predation in determining community structure, relation
between diversity and stability of ecological communities, energy
flow and nutrient cycling in major ecosystems. (Fall) Prerequisites:
BIOL 301 and BIOL 309 (also listed as STAT 350); MATH 151 is recommended.
and Quantitative Genetics
The emphasis in this course will be the study in natural populations
of characters whose variation is controlled by multiple genes. The
foundations in Mendelian and population genetics will be described,
followed by a comprehensive treatment of the field of quantitative
genetics and then by a discussion of the place of quantitative genetics
in behavioral genetics, physiological ecology, and in population
biology in general. Prerequisites: STAT 350 or BIOL 301 and BIOL
309 or consent of the instructor.
This course is designed to examine the basic properties of animal
viruses and bacteriophages with emphasis on the morphological and
biochemical aspects of viruses. Major topics will include virus-host
cell interaction, characterization and classification of viruses,
cultivation and quantitation of viruses, mechanisms of virus pathogenecity
and control. One hour each week will be devoted to discussion of
current literature in virology. Prerequisites: BIOL 302 and BIOL
303; BIOL 430 is recommended.
The biological characteristics of bacteria will be considered. Bacterial
physiology and ecology structure-function relationships, and host-parasite
interactions will be emphasized. Prerequisite: BIOL 303 or consent
of the instructor.
||Evolution: From Genes
This is a combined lecture and hands-on computing course comprising
four major sections that study the applications of evolutionary theory
to the exploration and analysis of phenotypic and biological
sequence data. We will begin by building a sound conceptual basis
theory of evolution, including an introduction to population
genetics. Real biological sequence data then will be introduced
to illustrate and extend this theory. From here, the focus will
shift to some major branches of current evolutionary research,
introducing recent published work for each topic. Finally, we
will stage a series of student-led debates that require application
of the science that has been learned to current socio-political
areas of controversy. Prerequisite: BIOL 301 and 302.
in Vertebrate Evolution
This course will provide a comprehensive survey treatment of the
five vertebrate classes, emphasizing paleontological approaches,
their morphological and behavioral adaptations, and evolution in
relation to climatic and geologic change. Both past and present
vertebrate communities will be considered. The laboratory component
of this course will stress species identification, biomechanics
and structure, and composition of past communities. Prerequisites:
BIOL 301 or consent of the instructor.
This course will provide a comprehensive review of current progress
of genetic engineering in plants, the applications of DNA technology
in crop improvement, using plants as energy, chemical and genetic
resources. Prospects of environmental impacts of terrestrial and
marine plants will also be discussed. Prerequisites: BIOL 302 and
Tutorial designed for nonthesis M.S. candidates to research and
write a scholarly paper in order to complete the requirements for
the non-thesis master's degree.
Seminar: Topics in Genetics
An examination of current problems in prokaryotic and eukaryotic
genetics. Such problems may include: transcription and translation,
recombination, DNA and chromosome replication, chromosome organization,
gene regulation, and extra chromosomal inheritance. Prerequisites:
Consent of the instructor, and passage of the genetics qualifying
examination. May be repeated for credit.