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This page contains a single entry from the blog posted on July 20, 2009 10:35 AM.

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MS Defense - Shelly Watts

Shelly successfully defended her masters thesis on July 20, 2009.

TITLE:
A study of Surface Plasmon-Coupled Emission from Rhodamine 6G using picosecond pulses

ABSTRACT:
Fluorescence measurements are used in life sciences to provide important information
about biomolecules (fluorophores) such as structure, mobility, and conformational
changes by detecting the target molecules on surfaces. Currently, fluorescence
measurements are performed using free-space (FS) detection, which are mostly isotropic,
resulting in detection of approximately 1% of the total emission. The emission process
may be limited by the background fluorescence due to its isotropic nature and,
photochemical destruction of the fluorophores.

Surface Plasmon-Coupled Emission (SPCE) is a fluorescence technique that has been
recently introduced that increases the fluorescence yield. SPCE is based on the
interaction of excited-state fluorophores with a nearby metal surface. The fluorophores
above the metal surface can couple with the plasmon resonances in the metal, resulting in
directional and wavelength-resolved emission. The coupled emission is characterized by
a dependence of the emission wavelength on the emission angle. In addition, the
emission is horizontally (p) polarized. An advantage of the SPCE over FS signal is the
reduction of the background fluorescence signal, since only fluorophores close to the
metal surface will couple to the surface plasmons.

Picosecond pulses were used to study the SPCE properties of Rhodamine 6G fluorophore
on a thin silver film. It is expected that using pulsed laser sources can greatly enhance
the SPCE signal over the FS signal. The SPCE signal is 3 times higher that the isotropic
FS signal. Thus, SPCE technique under pulsed excitation promises to be an effective tool
for fluorescence measurements in investigating the optical properties of biomolecules.

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