Synthetic Gene DataBase
 

Synthetic Gene 199


 
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Field NameNatural GeneSynthetic Gene
SGDB Gene ID185199
GenBank AccessionAF217189DQ289494
GenBank GI745355482880270
Gene NameepoDepoD(SYN)
Gene Length (bp)2174421834
SpeciesPolyangium cellulosum (Sorangium cellulosum)Escherichia coli
StrainsSMP44K207-3
CDSatgactactcgcggtcctacggcacagcagaatccgctgaaacaagcggccatcatcatt
cagcggctggaggagcggctcgctgggctcgcacaggcggagctggaacggaccgagccg
atcgccatcgtcggtatcggctgccgcttccctggcggtgcggacgctccggaagcgttt
tgggagctgctcgacgcggagcgcgacgcggtccagccgctcgacaggcgctgggcgctg
gtaggtgtcgctcccgtcgaggccgtgccgcactgggcggggctgctcaccgagccgata
gattgcttcgatgctgcgttcttcggcatctcgcctcgggaggcgcgatcgctcgacccg
cagcatcgtctgttgctggaggtcgcttgggaggggctcgaggacgccggtatcccgccc
cggtccatcgacgggagccgcaccggtgtgttcgtcggcgctttcacggcggactacgcg
cgcacggtcgctcggttgccgcgcgaggagcgagacgcgtacagcgccaccggcaacatg
ctcagcatcgccgccggacggctgtcgtacacgctggggctgcagggaccttgcctgacc
gtcgacacggcgtgctcgtcatcgctggtggcgattcacctcgcctgccgcagcctgcgc
gcaggagagagcgatctcgcgttggcgggaggggtcagcacgctcctctcccccgacatg
atggaagccgcggcgcgcacgcaagcgctgtcgcccgatggtcgttgccggaccttcgat
gcttcggccaacgggttcgtccgtggcgagggctgtggcctggtcgtcctcaaacggctc
tccgacgcgcaacgggatggcgaccgcatctgggcgctgatccggggctcggccatcaac
catgatggccggtcgaccgggttgaccgcgcccaacgtgctggctcaggagacggtcttg
cgcgaggcgctgcggagcgcccacgtcgaagctggggccgtcgattacgtcgagacccac
ggaacagggacctcgctgggcgatcccatcgaggtcgaggcgctgcgggcgacggtgggg
ccggcgcgctccgacggcacacgctgcgtgctgggcgcggtgaagaccaacatcggccat
ctcgaggccgcggcaggcgtagcgggcctgatcaaggcagcgctttcgctgacgcacgag
cgcatcccgagaaacctcaacttccgcacgctcaatccgcggatccggctcgagggcagc
gcgctcgcgttggcgaccgagccggtgccgtggccgcgcacggaccgtccgcgcttcgcg
ggggtgagctcgttcgggatgagcggaacgaacgcgcatgtggtgctggaagaggcgccg
gcggtggagctgtggcctgccgcgccggagcgctcggcggagcttttggtgctgtcgggc
aagagcgagggggcgctcgacgcgcaggcggcgcggctgcgcgagcacctggacatgcac
ccggagctcgggctcggggacgtggcgttcagcctggcgacgacgcgcagcgcgatgacc
caccggctcgcggtggcggtgacgtcgcgcgaggggctgctggcggcgctttcggccgtg
gcgcaggggcagacgccggcgggggcggcgcgctgcatcgcgagctcctcgcgcggcaag
ctggcgttgctgttcaccggacagggcgcgcagacgccgggcatgggccgggggctctgc
gcggcgtggccagcgttccgggaggcgttcgaccggtgcgtgacgctgttcgaccgggag
ctggaccgcccgctgcgcgaggtgatgtgggcggaggcggggagcgccgagtcgttgttg
ctggaccagacggcgttcacccagcccgcgctcttcgcggtggagtacgcgctgacggcg
ctgtggcggtcgtggggcgtagagccggagctcctggttgggcatagcatcggggagctg
gtggcggcgtgcgtggcgggggtgttctcgctggaagatggggtgaggctcgtggcggcg
cgcgggcggctgatgcaggggctctcggcgggcggcgcgatggtgtcgctcggagcgccg
gaggcggaggtggccgcggcggtggcgccgcacgcggcgtgggtgtcgatcgcggcggtc
aatgggccggagcaggtggtgatcgcgggcgtggagcaagcggtgcaggcgatcgcggcg
gggttcgcggcgcgcggcgtgcgcaccaagcggctgcatgtctcgcacgcgttccactcg
ccgctgatggaaccgatgctggaggagttcgggcgggtggcggcgtcggtgacgtaccgg
cggccaagcgtttcgctggtgagcaacctgagcgggaaggtggtcacggacgagctgagc
gcgccgggctactgggtgcggcacgtgcgggaggcggtgcgcttcgcggacggggtgaag
gcgctgcacgaagccggcgcgggcacgttcctcgaagtgggcccgaagccgacgctgctc
ggcctgttgccagcttgcctgccggaggcggagccgacgttgctggcgtcgttgcgcgcc
gggcgcgaggaggctgcgggggtgctcgaggcgctgggcaggctgtgggccgctggcggc
tcggtcagctggccgggcgtcttccccacggctgggcggcgggtgccgctgccgacctat
ccgtggcagcggcagcggtactggatcgaggcgccggccgaagggctcggagccacggcc
gccgatgcgctggcgcagtggttctaccgggtggactggcccgagatgcctcgctcatcc
gtggattcgcggcgagcccggtccggcgggtggctggtgctggccgaccggggtggagtc
ggggaggcggccgcggcggcgctttcgtcgcagggatgttcgtgcgccgtgctccatgcg
cccgccgaggcctccgcggtcgccgagcaggtgacccaggccctcggtggccgcaacgac
tggcagggggtgctgtacctgtggggtctggacgccgtcgtggaggcgggggcatcggcc
gaagaggtcggcaaagtcacccatcttgccacggcgccggtgctcgcgctgattcaggcg
gtgggcacggggccgcgctcaccccggctctggatcgtgacccgaggggcctgcacggtg
ggcggcgagcctgacgctgccccctgtcaggcggcgctgtggggtatgggccgggtcgcg
gcgctggagcatcccggctcctggggcgggctcgtggacctggatccggaggagagcccg
acggaggtcgaggccctggtggccgagctgctttcgccggacgccgaggatcagctggca
ttccgccaggggcgccggcgcgcagcgcggctcgtggccgccccaccggagggaaacgca
gcgccggtgtcgctgtctgcggaggggagttacttggtgacgggtgggctgggcgccctt
ggcctcctcgttgcgcggtggttggtggagcgcggggcggggcaccttgtgctgatcagc
cggcacggattgcccgaccgcgaggaatggggccgagatcagccgccagaggtgcgcgcg
cgcattgcggcgatcgaggcgctggaggcgcagggcgcgcgggtcaccgtggcggcggtc
gacgtggccgatgccgaaggcatggcggcgctcttggcggccgtcgagccgccgctgcgg
ggggtcgtgcacgccgcgggtctgctcgacgacgggctgctggcccaccaggacgccggt
cggctcgcccgggtgttgcgccccaaggtggagggggcatgggtgctgcacacccttacc
cgcgagcagccgctggacctcttcgtactgttttcctcggcgtcgggcgtcttcggctcg
atcggccagggcagctacgcggcaggcaatgcctttttggacgcgctggcggacctccgt
cgaacgcaggggctcgccgccctgagcatcgcctggggcctgtgggcggagggggggatg
ggctcgcaggcgcagcgccgggaacatgaggcatcgggaatctgggcgatgccgacgagt
cgtgccctggcggcgatggaatggctgctcggtacgcgcgcgacgcagcgcgtggtcatc
cagatggattgggcccatgcgggagcggctccgcgcgacgcgagccgaggccgcttctgg
gatcggctggtaactgtcacgaaagcggcctcctcctcggccgtgccagctgtagagcgc
tggcgcaacgcgtctgttgtggagacccgctcggcgctctacgagcttgtgcgcggcgtg
gtcgccggggtgatgggctttaccgaccaaggcacgctcgacgtgcgacgaggcttcgcc
gagcagggcctcgactccctgatggctgtggagatccgcaaacggcttcagggtgagctg
ggtatgccgctgtcggcgacgctggcgttcgaccatccgaccgtggagcggctggtggaa
tacttgctgagccaggcgctggagctgcaggaccgcaccgacgtgcgaagcgttcggttg
ccggcgacagaggacccgatcgccatcgtgggtgccgcctgccgcttcccgggcggggtc
gaggacctggagtcctactggcagctgttgaccgagggcgtggtggtcagcaccgaggtg
ccggccgaccggtggaatggggcagacgggcgcggccccggctcgggagaggctccgaga
cagacctacgtgcccaggggtggctttctgcgcgaggtggagacgttcgatgcggcgttc
ttccacatctcgcctcgggaggcgatgagcctggacccgcaacagcggctgctgctggaa
gtgagctgggaggcgatcgagcgcgcgggccaggacccgtcggcgctgcgcgagagcccc
acgggcgtgttcgtgggcgcgggccccaacgaatatgccgagcgggtgcaggacctcgcc
gatgaggcggcggggctctacagcggcaccggcaacatgctcagcgttgcggcgggacgg
ctgtcatttttcctgggcctgcacgggccgaccctggctgtggatacggcgtgctcctcg
tcgctcgtggcgctgcacctcggctgccagagcttgcgacggggcgagtgcgaccaagcc
ctggttggcggggtcaacatgctgctctcgccgaagaccttcgcgctgctctcacggatg
cacgcgctttcgcccggcgggcggtgcaagacgttctcggccgacgcggacggctacgcg
cgggccgagggctgcgccgtggtggtgctcaagcggctctccgacgcgcagcgcgaccgc
gaccccatcctggcggtgatccggggtacggcgatcaatcatgatggcccgagcagcggg
ctgacagtgcccagcggccctgcccaggaggcgctgttacgccaggcgctggcgcacgca
ggggtggttccggccgacgtcgatttcgtggaatgccacgggaccgggacggcgctgggc
gacccgatcgaggtgcgggcgctgagcgacgtgtacgggcaagcccgccctgcggaccga
ccgctgatcctgggagccgccaaggccaaccttgggcacatggagcccgcggcgggcctg
gccggcttgctcaaggcggtgctcgcgctggggcaagagcaaataccagcccagccggag
ctgggcgagctcaacccgctcttgccgtgggaggcgctgccggtggcggtggcccgcgca
gcggtgccgtggccgcgcacggaccgtccgcgcttcgcgggggtgagctcgttcgggatg
agcggaacgaacgcgcatgtggtgctggaagaggcgccggcggtggagctgtggcctgcc
gcgccggagcgctcggcggagcttttggtgctgtcgggcaagagcgagggggcgctcgac
gcgcaggcggcgcggctgcgcgagcacctggacatgcacccggagctcgggctcggggac
gtggcgttcagcctggcgacgacgcgcagcgcgatgaaccaccggctcgcggtggcggtg
acgtcgcgcgaggggctgctggcggcgctttcggccgtggcgcaggggcagacgccgccg
ggggcggcgcgctgcatcgcgagctcgtcgcgcggcaagctggcgttcctgttcaccgga
cagggcgcgcagacgccgggcatgggccgggggctttgcgcggcgtggccagcgttccga
gaggcgttcgaccggtgcgtggcgctgttcgaccgggagctggaccgcccgctgtgcgag
gtgatgtgggcggagccggggagcgccgagtcgttgttgctcgaccagacggcgttcacc
cagcccgcgctcttcacggtggagtacgcgctgacggcgctgtggcggtcgtggggcgta
gagccggagctggtggctgggcatagcgccggggagctggtggcggcgtgcgtggcgggg
gtgttctcgctggaagatggggtgaggctcgtggcggcgcgcgggcggctgatgcagggg
ctctcggcgggcggcgcgatggtgtcgctcggagcgccggaggcggaggtggccgcggcg
gtggcgccgcacgcggcgtgggtgtcgatcgcggcggtcaatgggccggagcaggtggtg
atcgcgggcgtggagcaagcggtgcaggcgatcgcggcggggttcgcggcgcgcggcgtg
cgcaccaagcggctgcatgtctcgcacgcatcccactcgccgctgatggaaccgatgctg
gaggagttcgggcgggtggcggcgtcggtgacgtaccggcggccaagcgtttcgctggtg
agcaacctgagcgggaaggtggtcacggacgagctgagcgcgccgggctactgggtgcgg
cacgtgcgggaggcggtgcgcttcgcggacggggtgaaggcgctgcacgaagccggcgcg
gggacgttcctcgaagtgggcccgaagccgacgctgctcggcctgttgccagcttgcctg
ccggaggcggagccgacgctgctggcgtcgttgcgcgccgggcgcgaggaggctgcgggg
gtgctcgaggcgctgggcaggctgtgggccgccggcggctcggtcagctggccgggcgtc
ttccccacggctgggcggcgggtgccgctgccgacctatccgtggcagcggcagcggtac
tggcccgacatcgagcctgacagccgtcgccacgcagccgcggatccgacccaaggctgg
ttctatcgcgtggactggccggagatacctcgcagcctccagaaatcagaggaggcgagc
cgcgggagctggctggtattggcggataagggtggagtcggcgaggcggtcgctgcagcg
ctgtcgacacgtggacttccatgcgtcgtgctccatgcgccggcagagacatccgcgacc
gccgagctggtgaccgaggctgccggcggtcgaagcgattggcaggtagtgctctacctg
tggggtctggacgccgtcgtcggcgcggaggcgtcgatcgatgagatcggcgacgcgacc
cgtcgtgctaccgcgccggtgctcggcttggctcggtttctgagcaccgtgtcttgttcg
ccccgactctgggtcgtgacccggggggcatgcatcgttggcgacgagcctgcgatcgcc
ccttgtcaggcggcgttatggggcatgggccgggtggcggcgctcgagcatcccggggcc
tggggcgggctcgtggacctggatccccgagcgagcccgccccaagccagcccgatcgac
ggcgagatgctcgtcaccgagctattgtcgcaggagaccgaggaccagctcgccttccgc
catgggcgccggcacgcggcacggctggtggccgccccgccacggggggaagcggcaccg
gcgtcgctgtctgcggaggcgagctacctggtgacgggaggcctcggtgggctgggcctg
atcgtggcccagtggctggtggagctgggagcgcggcacttggtgctgaccagccggcgc
gggttgcccgaccggcaggcgtggcgcgagcagcagccgcctgagatccgcgcgcggatc
gcagcggtcgaggcgctggaggcgcggggtgcacgggtgaccgtggcagcggtggacgtg
gccgacgtcgaaccgatgacagcgctggtttcgtcggtcgagcccccgctgcgaggggtg
gtgcacgccgctggcgtcagcgtcatgcgtccactggcggagacggacgagaccctgctc
gagtcggtgctccgtcccaaggtggccgggagctggctgctgcaccggctgctgcacggc
cggcctctcgacctgttcgtgctgttctcgtcgggcgcagcggtgtggggtagccatagc
cagggtgcgtacgcggcggccaacgctttcctcgacgggctcgcgcatcttcggcgttcg
caatcgctgcctgcgttgagcgtcgcgtggggtctgtgggccgagggaggcatggcggac
gcggaggctcatgcacgtctgagcgacatcggggttctgcccatgtcgacgtcggcagcg
ttgtcggcgctccagcgcctggtggagaccggcgcggctcagcgcacggtgacccggatg
gactgggcgcgcttcgcgccggtgtacaccgctcgagggcgtcgcaacctgctttcggcg
ctggtcgcagggcgcgacatcatcgcgccttcccctccggcggcagcaacccggaactgg
cgtggcctgtccgttgcggaagcccgcatggctctgcacgaggtcgtccatggggccgtc
gctcgggtgctgggcttcctcgacccgagcgcgctcgatcctgggatggggttcaatgag
cagggcctcgactcgttgatggcggtggagatccgcaacctccttcaggctgagctggac
gtgcggctttcgacgacgctggcctttgatcatccgacggtacagcggctggtggagcat
ctgctcgtcgatgtactgaagctggaggatcgcagcgacacccagcatgttcggtcgttg
gcgtcagacgagcccatcgccatcgtgggagccgcctgccgcttcccgggcggggtggag
gacctggagtcctactggcagctgttggccgagggcgtggtggtcagcgccgaggtgccg
gccgaccggtgggatgcggcggactggtacgaccctgatccggagatcccaggccggact
tacgtgaccaaaggcgccttcctgcgcgatttgcagagattggatgcgaccttcttccgc
atctcgcctcgcgaggcgatgagcctcgacccgcagcagcggttgctcctggaggtaagc
tgggaggcgctcgagagcgcgggtatcgctccggatacgctgcgagatagccccaccggg
gtgttcgtgggtgcggggcccaatgagtactacacgcagcggctgcgaggcttcaccgac
ggagcggcagggctgtacggcggcaccgggaacatgctcagcgttgcggctggacggctg
tcgtttttcctgggtctgcacggcccgacgctggccatggatacggcgtgctcgtcctcc
ctggtcgcgctgcacctcgcctgccagagcctgcgactgggcgagtgcgatcaagcgctg
gttggcggggtcaacgtgctgctcgcgccggagaccttcgtgctgctctcacggatgcgc
gcgctttcgcccgacgggcggtgcaagacgttctcggccgacgcggacggctacgcgcgg
ggcgaggggtgcgccgtggtggtgctcaagcggctgcgcgatgcgcagcgcgccggcgac
tccatcctggcgctgatccggggaagcgcggtgaaccacgacggcccgagcagcgggctg
accgtgcccaacggacccgcccagcaagcattgctgcgccaggcgctttcgcaagcaggc
gtgtctccggtcgacgttgattttgtggagtgtcacgggacagggacggcgctgggcgac
ccgatcgaggtgcaggcgctgagcgaggtgtatggtccagggcgctccgaggatcgaccg
ctggtgctgggggccgtcaaggccaacgtcgcgcatctggaggcggcatccggcttggcc
agcctgctcaaggccgtgcttgcgctgcggcacgagcagatcccggcccagccggagctg
ggggagctcaacccgcacttgccgtggaacacgctgccggtggcggtgccacgtaaggcg
gtgccgtgggggcgcggcgcacggccgcgtcgggccggcgtgagcgcgttcgggttgagc
ggaaccaacgtgcatgtcgtgctggaggaggcaccggaggtggagctggtgcccgcggcg
ccggcgcgaccggtggagctggttgtgctatcggccaagagcgcggcggcgctggacgcc
gcggcggaacggctctcggcgcacctgtccgcgcacccggagctgagcctcggcgacgtg
gcgttcagcctggcgacgacgcgcagcccgatggagcaccggctcgccatcgcgacgacc
tcgcgcgaggccctgcgaggcgcgctggacgccgcggcgcagcggcagacgccgcagggc
gcggtgcgcggcaaggccgtgtcctcacgcggtaagttggctttcctgttcaccggacag
ggcgcgcaaatgccgggcatgggccgtgggctgtacgaggcgtggccagcgttccgggag
gcgttcgaccggtgcgtggcgctcttcgatcgggagctcgaccagcctctgcgcgaggtg
atgtgggctgcgccgggcctcgctcaggcggcgcggctcgatcagaccgcgtacgcgcag
ccggctctctttgcgctggagtacgcgctggctgccctgtggcgttcgtggggcgtggag
ccgcacgtactcctcggtcatagcatcggcgagctggtcgccgcctgcgtggcgggcgtg
ttctcgctcgaagacgcggtgaggttggtggccgcgcgcgggcggctgatgcaggcgctg
cccgccggcggtgccatggtcgccatcgcagcgtccgaggccgaggtggccgcctccgtg
gcaccccacgccgccacggtgtcgatcgccgcggtcaacggtcctgacgccgtcgtgatc
gctggcgccgaggtacaggtgctcgccctcggcgcgacgttcgcggcgcgtgggatacgc
acgaagaggctcgccgtctcccatgcgttccactcgccgctcatggatccgatgctggaa
gacttccagcgggtcgctgcgacgatcgcgtaccgcgcgccagaccgcccggtggtgtcg
aatgtcaccggccacgtcgcaggccccgagatcgccacgcccgagtattgggtccggcat
gtgcgaagcgccgtgcgcttcggcgatggggcaaaggcgttgcatgccgcgggtgccgcc
acgttcgtcgagattggcccgaagccggtcctgctcgggctattgccagcgtgcctcggg
gaagcggacgcggtcctcgtgccgtcgctacgcgcggaccgctcggaatgcgaggtggtc
ctcgcggcgctcgggacttggtatgcctgggggggtgcgctcgactggaagggcgtgttc
cccgatggcgcgcgccgcgtggctctgcccatgtatccatggcagcgtgagcgccattgg
atggacctcaccccgcgaagcgccgcgcctgcagggatcgcaggtcgctggccgctggct
ggtgtcgggctctgcatgcccggcgctgtgttgcaccacgtgctctcgatcggaccacgc
catcagcccttcctcggtgatcacctcgtgtttggcaaggtggtggtgcccggcgccttt
catgtcgcggtgatcctcagcatcgccgccgagcgctggcccgagcgggcgatcgagctg
acaggcgtggagttcctgaaggcgatcgcgatggagcccgaccaggaggtcgagctccac
gccgtgctcacccccgaagccgccggggatggctacctgttcgagctggcgaccctggcg
gcgccggagaccgaacgccgatggacgacccacgcccgcggtcgggtgcagccgacagac
ggcgcgcccggcgcgttgccgcgcctcgaggtgctggaggaccgcgcgatccagcccctc
gacttcgccggattcctcgacaggttatcggcggtgcggatcggctggggtccgctttgg
cgatggctgcaggacgggcgcgtcggcgacgaggcctcgcttgccaccctcgtgccgacc
tatccgaacgcccacgacgtggcgcccttgcacccgatcctgctggacaacggctttgcg
gtgagcctgctggcaacccggagcgagccggaggacgacgggacgcccccgctgccgttc
gccgtggaacgggtgcggtggtggcgggcgccggttggaagggtgcggtgtggcggcgtg
ccgcggtcgcaggcattcggtgtctcgagcttcgtgctggtcgacgaaactggcgaggtg
gtcgctgaggtggagggatttgtttgccgccgggcgccgcgagaggtgttcctgcggcag
gagtcgggcgcgtcgactgcagccttgtaccgcctcgactggcccgaagcccccttgccc
gatgcgcctgcggaacggatggaggagagctgggtcgtggtggcagcacctggctcggag
atggccgcggcgctcgcaacacggctcaaccgctgcgtactcgccgaacccaaaggcctc
gaggcggccctcgcgggggtgtctcccgcaggtgtgatctgcctctgggaacctggagcc
cacgaggaagctccggcggcggcgcagcgtgtggcgaccgagggcctttcggtggtgcag
gcgctcagggatcgcgcggtgcgcctgtggtgggtgaccacgggcgccgtggctgtcgag
gccggtgagcgggtgcaggtcgccacagcgccggtatggggcctgggccggacagtgatg
caggagcgcccggagctcagctgcactctggtggatttggagccggaggtcgatgccgcg
cgttcagctgacgttctgctgcgggagctcggtcgcgctgacgacgagacccaggtggtt
ttccgttccggagagcgccgcgtagcgcggctggtcaaagcgacaacccccgaagggctc
ttggtccctgacgcagaatcctatcgactggaggctgggcagaagggcacattggaccag
ctccgcctcgcgccggcacagcgccgggcacccggcccgggcgaggtcgagatcaaggta
accgcctcggggctcaacttccggaccgtcctcgctgtgctgggaatgtatccgggcgac
gctgggccgatgggcggagattgtgccggtatcgtcacggcggtgggccagggggtgcac
cacctctcggtcggcgatgctgtcatgacgctggggacgttgcatcgattcgtcacggtc
gacgcgcggctggtggtccggcagcctgcagggctgactcccgcgcaggcagctacggtg
ccggttgcgttcctgacggcctggctcgctctgcacgacctggggaatctgcggcgcggc
gagcgggtgctgatccatgctgcggccggcggcgtgggcatggccgcggtgcaaatcgcc
cgatggataggggccgaggtgttcgccacggcgagcccgtccaagtgggcagcggttcag
gccatgggcgtgccgcgcacgcacatcgccagctcgcggacgctggagtttgctgagacg
ttccggcaggtcaccggcggccggggcgtggacgtggtgctcaacgcgctggccggcgag
ttcgtggacgcgagcctgtccctgctgacgacgggcgggcggttcctcgagatgggcaag
accgacatacgggatcgagccgcggtcgcggcggcgcatcccggtgttcgctatcgggta
ttcgacatcctggagctcgctccggatcgaactcgagagatcctcgagcgcgtggtcgag
ggctttgctgcgggacatctgcgcgcattgccggtgcatgcgttcgcgatcaccaaggcc
gaggcagcgtttcggttcatggcgcaagcgcggcatcagggcaaggtcgtgctgctgccg
gcgccctccgcagcgcccttggcgccgacgggcaccgtactgctgaccggtgggctggga
gcgttggggctccacgtggcccgctggctcgcccagcagggcgcgccgcacatggtgctc
acaggtcggcggggcctggatacgccgggcgctgccaaagccgtcgcggagatcgaagcg
ctcggcgctcgggtgacgatcgcggcgtcggatgtcgccgatcggaacgcgctggaggct
gtgctccaggccattccggcggagtggccgttacagggcgtgatccatgcagccggagcg
ctcgatgatggtgtgcttgatgagcagaccaccgaccgcttctcgcgggtgctggcaccg
aaggtgactggcgcctggaatctgcatgagctcacggcgggcaacgatctcgctttcttc
gtgctgttctcctccatgtcggggctcttgggctcggccgggcagtccaactatgcggcg
gccaacaccttcctcgacgcgctggccgcgcatcggcgggccgaaggcctggcggcgcag
agcctcgcgtggggcccatggtcggacggaggcatggcagcggggctcagcgcggcgctg
caggcgcggctcgctcggcatgggatgggagcgctgtcgcccgctcagggcaccgcgctg
ctcgggcaggcgctggctcggccggaaacgcagctcggggcgatgtcgctcgacgtgcgt
gcggcaagccaagcttcgggagcggcagtgccgcctgtgtggcgcgcgctggtgcgcgcg
gaggcgcgccatgcggcggctggggcgcagggggcattggccgcgcgccttggggcgctg
cccgaggcgcgtcgcgccgacgaggtgcgcaaggtcgtgcaggccgagatcgcgcgcgtg
ctttcatggggcgccgcgagcgccgtgcccgtcgatcggccgctgtcggacttgggcctc
gactcgctcacggcggtggagctgcgcaacgtgctcggccagcgggtgggtgcgacgctg
ccggcgacgctggcattcgatcacccgacggtcgacgcgctcacgcgctggctgctcgat
aaggtcctggccgtggccgagccgagcgtatcgcccgcaaagtcgtcgccgcaggtcgcc
ctcgacgagcccattgcggtgatcggcatcggctgccgtttcccaggcggcgtgaccgat
ccggagtcgttttggcggctgctcgaagagggcagcgatgccgtcgtcgaggtgccgcat
gagcgatgggacatcgacgcgttctatgatccggatccggatgtgcgcggcaagatgacg
acacgctttggcggcttcctgtccgatatcgaccggttcgagccggccttcttcggcatc
tcgccgcgcgaagcgacgaccatggatccgcagcagcggctgctcctggagacgagctgg
gaggcgttcgagcgcgccgggattttgcccgagcggctgatgggcagcgataccggcgtg
ttcgtggggctcttctaccaggagtacgctgcgctcgccggcggcatcgaggcgttcgat
ggctatctaggcaccggcaccacggccagcgtcgcctcgggcaggatctcttatgtgctc
gggctaaaggggccgagcctgacggtggacaccgcgtgctcctcgtcgctggtcgcggtg
cacctggcctgccaggcgctgcggcggggcgagtgttcggtggcgctggccggcggcgtg
gcgctgatgctcacgccggcgacgttcgtggagttcagccggctgcgaggcctggctccc
gacggacggtgcaagagcttctcggccgcagccgacggcgtggggtggagcgaaggctgc
gccatgctcctgctcaaaccgcttcgcgatgctcagcgcgatggggatccgatcctggcg
gtgatccgcggcaccgcggtgaaccaggatgggcgcagcaacgggctgacggcgcccaac
gggtcgtcgcagcaagaggtgatccgtcgggccctggagcaggcggggctggctccggcg
gacgtcagctacgtcgagtgccacggcaccggcacgacgttgggcgaccccatcgaagtg
caggccctgggcgccgtgctggcacaggggcgaccctcggaccggccgctcgtgatcggg
tcggtgaagtccaatatcggacatacgcaggctgcggcgggcgtggccggtgtcatcaag
gtggcgctggcgctcgagcgcgggcttatcccgaggagcctgcatttcgacgcgcccaat
ccgcacattccgtggtcggagctcgccgtgcaggtggccgccaaacccgtcgaatggacg
agaaacggcgcgccgcgacgagccggggtgagctcgtttggcgtcagcgggaccaacgcg
cacgtggtgctggaggaggcgccagcggcggcgttcgcgcccgcggcggcgcgttcagcg
gagcttttcgtgctgtcggcgaagagcgccgcggcgctggacgcgcaggcggcgcggctt
tcggcgcatgtcgttgcgcacccggagctcggcctcggcgacctggcgttcagcctggcg
acgacccgcagcccgatgacgtaccggctcgcggtggcggcgacctcgcgcgaggcgctg
tctgcggcgctcgacacagcggcgcaggggcaggcgccgcccgcagcggctcgcggccac
gcttccacaggcagcgccccaaaggtggttttcgtctttcctggccagggctcccagtgg
ctgggcatgggccaaaagctcctctcggaggagcccgtcttccgcgacgcgctctcggcg
tgtgaccgagcgattcaggccgaagccggctggtcgctgctcgccgagctcgcggccgat
gagaccacctcgcagctcggccgcatcgacgtggtgcagccggcgctgttcgcgatcgag
gtcgcgctgtcggcgctgtggcggtcgtggggcgtcgagccggatgcagtggtaggccac
agcatgggcgaagtggcggccgcgcacgtcgccggcgccctgtcgctcgaggatgctgta
gcgatcatctgccggcgcagcctgctgctgcggcggatcagcggccaaggcgagatggcg
gtcgtcgagctctccctggccgaggccgaggcagcgctcctgggctacgaagatcggctc
agcgtggcggtgagcaacagcccgcgatcgacggtgctggcgggcgagccggcagcgctc
gcagaggtgctggcgatccttgcggcaaagggggtgttctgccgtcgagtcaaggtggac
gtcgccagccacagcccacagatcgacccgctgcgcgacgagctattggcagcattgggc
gagctcgagccgcgacaagcgaccgtgtcgatgcgctcgacggtgacgagcacgatcgtg
gcgggcccggagctcgtggcgagctactgggcggacaacgttcgacagccggtgcgcttc
gccgaagcggtgcaatcgttgatggaaggcggtcatgggctgttcgtggagatgagcccg
catccgatcctgacgacgtcggtcgaggagatccgacgggcgacgaagcgggagggagtc
gcggtgggctcgttgcggcgtggacaggacgagcgcctgtccatgttggaggcgctggga
gcgctctgggtacacggccaggcggtgggctgggagcggctgttctccgcgggcggcgcg
ggcctccgtcgcgtgccgctgccgacctatccctggcagcgcgagcggtactgggtcgaa
gcgccgaccggcggcgcggcgagcggcagccgctttgctcatgcgggcagtcacccgctc
ctgggtgaaatgcagaccctgtcgacccagaggagcacgcgcgtgtgggagacgacgctg
gatctcaaacggctgccgtggctcggcgatcaccgggtgcagggggcggtcgtgttcccg
ggcgcggcgtacctggagatggcgctttcgtctggggccgaggccttgggtgacggtccg
ctccaggtcagcgatgtggtgctcgccgaggcgctggccttcgcggatgatacgccggtg
gcggtgcaggtcatggcgaccgaggagcgaccaggccgcctgcaattccacgttgcgagc
cgggtgccgggccacggccgtgctgcctttcgaagccatgcccgcggggtgctgcgccag
accgagcgcgccgaggtcccggcgaggctggatctggccgcgcttcgtgcccggcttcag
gccagcgcacccgctgcggctacctatgcggcgctggccgagatggggctcgagtacggc
ccagcgttccaggggcttgtcgagctgtggcggggggagggcgaggcgctgggacgtgtg
cggctccccgaggccgccggctccccagccgcgtgccggctccaccccgcgctcttggat
gcgtgcttccacgtgagcagcgccttcgctgaccgcggcgaggcgacgccatgggtaccc
gtcgaaatcggctcgctgcggtggttccagcggccgtcgggggagctgtggtgtcatgcg
cggagcgtgagccacggaaagccaacacccgatcggcggagtaccgacttttgggtggtc
gacagcacgggcgcgatcgtcgccgagatctccgggctcgtggcgcagcggctcgcggga
ggtgtacgccggcgcgaagaagacgactggttcatggagccggcttgggaaccgaccgcg
gtccccggatccgaggtcacggcgggccggtggctgctcatcggctcgggcggcgggctc
ggcgctgcgctctactcggcgctgacggaagctggccattccgtcgtccacgcgacaggg
cacggcacgagcgccgccgggttgcaggcactcctgacggcgtccttcgacggccaggcc
ccgacgtcggtggtgcacctcggcagcctcgatgagcgtggcgtgctcgacgcggatgcc
cccttcgacgccgatgccctcgaggagtcgctggtgcgcggctgcgacagcgtgctctgg
accgtgcaggccgtggccggggcgggcttccgagatcctccgcggttgtggctcgtgaca
cgcggcgctcaggccatcggcgccggcgacgtctccgtggcgcaagcgccgctcctgggg
ctgggccgcgttatcgccttggagcacgccgagctgcgctgcgctcggatcgacctcgat
ccagcgcggcgcgacggagaggtcgatgagctgcttgccgagctgttggccgacgacgcc
gaggaggaagtcgcgtttcgcggcggtgagcggcgcgtggcccggctcgtccgaaggctg
cccgagaccgactgccgagagaaaatcgagcccgcggaaggccggccgttccggctggag
atcgatgggtccggcgtgctcgacgacctggtgctccgagccacggagcggcgccctcct
ggcccgggcgaggtcgagatcgccgtcgaggcggcggggctcaactttctcgacgtgatg
agggccatggggatctaccctgggcccggggacggtccggttgcgctgggcgccgagtgc
tccggccgaattgtcgcgatgggcgaaggtgtcgagagccttcgtatcggccaggacgtc
gtggccgtcgcgcccttcagtttcggcacccacgtcaccatcgacgcccggatggtcgca
cctcgccccgcggcgctgacggccgcgcaggcagccgcgctgcccgtcgcattcatgacg
gcctggtacggtctcgtccatctggggaggctccgggccggcgagcgcgtgctcatccac
tcggcgacggggggcaccgggctcgctgctgtgcagatcgcccgccacctcggcgcggag
atatttgcgaccgctggtacgccggagaagcgggcgtggctgcgcgagcaggggatcgcg
cacgtgatggactcgcggtcgctggacttcgccgagcaagtgctggccgcgacgaagggc
gagggggtcgacgtcgtgttgaactcgctgtctggcgccgcgatcgacgcgagccttgcg
accctcgtgccggacggccgcttcatcgagctcggcaagacggacatctatgcagatcgc
tcgctggggctcgctcactttaggaagagcctgtcctacagcgccgtcgatcttgcgggt
ttggccgtgcgtcggcccgagcgcgtcgcagcgctgctggcggaggtggtggacctgctc
gcacggggagcgctgcagccgcttccggtagagatcttccccctctcgcgggccgcggac
gcgttccggaaaatggcgcaagcgcagcatctcgggaagctcgtgctcgcgctggaggac
ccggacgtgcggatccgcgttccgggcgaatccggcgtcgccatccgcgcggacggcacc
tacctcgtgaccggcggtctgggtgggctcggtctgagcgtggctggatggctggccgag
cagggggctgggcatctggtgctggtgggccgctccggtgcggtgagcgcggagcagcag
acggctgtcgccgcgctcgaggcgcacggcgcgcgtgtcacggtagcgagggcagacgtc
gccgatcgggcgcagatcgagcggatcctccgcgaggttaccgcgtcggggatgccgctc
cgcggcgtcgttcatgcggccggtatcctggacgacgggctgctgatgcagcaaaccccc
gcgcggttccgcgcggtcatggcgcccaaggtccgaggggccttgcacctgcatgcgttg
acacgcgaagcgccgctctccttcttcgtgctgtacgcttcgggagcagggctcttgggc
tcgccgggccagggcaactacgccgcggccaacacgttcctcgacgctctggcacaccac
cggagggcgcaggggctgccagcattgagcatcgactggggcctgttcgcggacgtgggt
ttggccgccgggcagcaaaatcgcggcgcacggctggtcacccgcgggacgcggagcctc
acccccgacgaagggctgtgggcgctcgagcgtctgctcgacggcgatcgcacccaggcc
ggggtcatgccgttcgacgtgcggcagtgggtggagttctacccggcggcggcatcttcg
cggaggttgtcgcggctggtgacggcacggcgcgtggcttccggtcggctcgccggggat
cgggacctgctcgaacggctcgccaccgccgaggcgggcgcgcgggcaggaatgctgcag
gaggtcgtgcgcgcgcaggtctcgcaggtgctgcgcctccccgaaggcaagctcgacgtg
gatgcgccgctcacgagcctgggaatggactcgctgatggggctagagctgcgcaaccgc
atcgaggccgtgctcggcatcaccatgccggcgaccctgctgtggacctaccccacggtg
gcagcgctgagtgcgcatctggcttctcatgtcgtctctacgggggatggggaatccgcg
cgcccgccggatacagggaacgtggctccaatgacccacgaagtcgcttcgctcgacgaa
gacgggttgttcgcgttgattgatgagtcactcgcgcgtgcgggaaagaggtga
atgaccacacgtggcccgaccgctcaacaaaatccactgaaacaagcagcaattatcatt
cagcgccttgaagaacgccttgcaggtctggcacaagcggaactggagcgtactgagcca
attgcgatcgtaggcatcgggtgtcgttttccgggtggcgcagacgcgccggaagcattc
tgggaactgctcgatgctgagcgcgatgccgttcagcctttggaccgtcgctgggcactg
gtcggggtagcgccagtggaagcggtccctcattgggcgggtttattgaccgaaccgatt
gactgtttcgatgcggccttttttggtatttcgccgcgtgaagcacgtagcttggatccg
cagcaccgtctgctccttgaagtagcatgggaggggctggaagacgccggcatcccaccg
cgtagcattgacggctctcgcactggtgtctttgtgggtgcgttcaccgccgattatgcc
cgtactgttgctcgcctgcctcgtgaagaacgcgacgcgtacagcgcgacaggtaacatg
ttatccatcgcggctgggcgtttgtcgtatacgttgggcctccagggcccgtgtttgacc
gttgataccgcatgctcgtcctctcttgttgctattcatctggcgtgccgctccttgcgg
gctggcgaaagtgacctggcccttgcaggcggcgtctcgacgttgttatcacctgatatg
atggaagcggcggcacgcacccaggccctgtccccggatggccgctgtcgtactttcgat
gcgtcggcgaatggctttgtacgtggtgagggttgtggtctggtcgttctcaaacgttta
tccgacgcacagcgtgacggcgaccgtatttgggcgttaatccgcggctcagcgattaat
catgacggtcgctccacgggcctgacagcgccgaacgtccttgcgcaggaaacggtgctg
cgcgaagcactgcgtagtgcgcacgttgaagcaggggccgtggattacgtggagactcat
ggcaccggcaccagcctgggcgatccgatcgaagtggaggccctgagagccaccgtcggc
ccagcccggagcgacggtactcgctgtgtgttaggcgcggtaaaaacgaacattggacac
ctggaggcagccgctggtgtagctgggctgattaaagctgcgctgtccttaacgcacgaa
cgcatcccgcgtaacctgaactttcgtaccttgaacccgcgtatccgtcttgaaggctct
gcattggcgctcgcaaccgagccagttccttggccgcgcacagatcgcccacgctttgcc
ggtgtgagttcatttggcatgtcgggtaccaatgctcacgtggtactggaggaggctccg
gccgtggaactgtggcctgcggcgccggaacgttccgctgaactgctggtgctgagcggc
aaatctgaaggtgccctggatgctcaagctgcccgtctgcgtgaacatttggacatgcac
ccggaactggggttaggcgatgtggctttctccctggcaacgacccgctctgcgatgaca
catcggttggctgttgcggtaacctcccgcgaaggtctgttggccgccttgtcagcggtt
gcacagggccaaacgccagcaggcgctgcacggtgcattgcgagctctagtcgcggtaag
ctggctctgctgtttactggccagggcgcccaaactccgggtatgggtcgcggcttatgt
gccgcctggcccgcttttcgtgaagcctttgatcgctgtgtaacgttatttgaccgtgag
ctggatcggccactgcgggaggttatgtgggcggaagctgggtccgccgaatcattactg
ttagaccagaccgcgttcacgcagcccgcgctgttcgctgtcgaatatgccctgacggcg
ctctggagatcttggggtgtcgaaccagaactgctggttggacactctattggcgaactg
gtcgcggcgtgcgtggctggcgttttctctcttgaagacggtgtgcgcctcgtggcggct
cggggtcgcctcatgcaggggctgagcgctggcggcgccatggtgtcactgggtgctcca
gaggcagaagtagcagcagccgtcgcaccacatgcggcatgggtttcaatcgccgccgta
aatggcccagagcaggtagttattgcaggcgtcgaacaagcggtgcaggcaatcgccgca
gggtttgcggcgcgcggcgtgcgcactaaacgcctccacgtctctcatgcctttcactcc
ccgctgatggaaccaatgctggaagagttcggtcgcgtggcagcgtctgttacctaccgt
cgtcctagcgtctcgctcgtttccaacctgagtggtaaagtggttactgacgagctgagc
gccccaggctactgggttcgtcatgtgcgcgaagccgtccgttttgctgatggtgtgaaa
gccctgcacgaagcgggcgcgggcacctttctggaagtcggtccgaaaccaaccctgctg
ggcctgctcccggcgtgcctgccagaagcagaacctacgttattagcgagcttgcgggcg
ggccgtgaagaagcagcgggtgttctggaggcccttgggcgtttgtgggcggcaggcggt
tccgtttcttggcctggcgtttttccaaccgctggtcgccgtgtgccgcttccgacctat
ccgtggcaacgtcagcgctattggctgcaggcaccggcggaagggctgggtgcgactgcg
gcagatgcgttagcccagtggttttatcgcgtggattggccggaaatgccacggagtagc
gttgattctcgccgtgcgcgttcgggcggctggcttgtcctggcggaccgtggcggggtg
ggcgaagcagccgcagcggcactgagtagtcaaggctgctcatgtgcggtgttacatgct
ccggcggaggcgtccgccgtcgccgaacaggtgacccaggccctgggcgggcgcaatgat
tggcagggcgttctgtacttgtggggtctggatgcagtcgtcgaggcgggcgcatccgca
gaggaggtgggtaaagtgacacacctggcgaccgctccggtgttagcactgattcaggcc
gtcgggactggcccgcgcagccctcgcctgtggattgtaacgcgtggggcttgtacggtc
ggtggcgagccggatgctgccccgtgtcaggctgcactgtgggggatgggtcgtgtggca
gccttggaacatccgggctcctggggtggtctggttgatctggatccggaagaatctcca
acggaagtagaagcgctggtggctgaactgctgtctccggatgccgaagatcagctcgca
tttcgtcaaggccgtcgtcgtgccgcccgcttggtcgccgcgccaccggagggcaacgca
gcgccggtgtcgttaagcgcggaaggttcatatttggttaccggtggtctgggcgctctg
ggtctgctggtggctcgctggctggtggaacgtggtgcgggtcatctggttttaatctct
cggcacgggcttcctgatcgcgaagaatggggccgtgatcaaccacctgaggtacgggcc
cgtatcgcagcgattgaggccctcgaagctcaaggcgcacgcgtaacggttgccgccgtg
gatgttgcagacgctgaggggatggccgctcttttagcagccgtggagccgccactgcgc
ggcgtggtccatgccgctggcctgctggacgacggtctgttagcgcaccaggatgcaggt
cgcctggctcgggtgttacgtccgaaagttgaaggtgcttgggttctgcataccctgacc
cgcgagcagcctcttgatctgtttgttctgtttagctccgcaagtggtgttttcggttcc
atcggccagggctcttatgcggcagggaacgcatttttggatgctctggcggatctgcgt
cgtacacaaggcttggcggccttaagcattgcatggggcctgtgggcggaagggggtatg
ggctcacaagcccagcgccgcgagcatgaggcatccggtatctgggcgatgccgacgtct
cgcgccctggcggcaatggaatggctcctgggcacccgcgccacgcagcgtgtggtaatt
cagatggactgggctcacgcgggtgcagcaccacgggatgcttccagagggcgtttctgg
gatcgtctcgtaaccgtcaccaaagcagctagtagcagtgctgtgcccgcagttgaacgc
tggcgtaatgcaagcgtggtcgaaacccgttcggctctgtatgagctggtgcgcggcgtg
gtagcaggtgtgatgggttttactgatcaaggcacattagatgtccggcgcggctttgca
gagcagggtttagatagcctcatggcggttgaaattcgtaaacgtctgcaaggcgagctg
ggtatgccgttgtctgccacattggcgttcgatcatccgaccgtagaacgtttggtggaa
tatttacttagccaagcgtctagtttacaggaccgtacggatgtccgctccgtgcgtctg
ccagcaacggaagatccaattgcgattgttggggcggcatgccgttttccgggtggcgtc
gaggacctggaatcttactggcagttgctgacggaaggtgtggtcgtttctaccgaagta
ccggcagaccgttggaacggggcggacggccgtggccctggcagcggtgaagcaccgcgc
cagacctatgtcccgcgcggtggctttctccgcgaagtcgaaacttttgacgcggccttc
tttcacatctctccgcgtgaagctatgtccctggacccgcagcaacgcctgttgttagaa
gtctcgtgggaagcaatcgaacgtgccggccaggatccgagtgccctgcgtgaatctcct
actggagtgtttgtgggtgcgggcccgaatgagtatgcagaacgtgttcaggacttagct
gatgaagcagcagggctctactccggaactggcaatatgctgagcgtcgcggcagggcgt
ctttccttttttttggggttacacggcccgaccctggcagtcgacactgcctgtagtagc
agtctggtcgcgttgcaccttggctgtcaatcactgcgccgtggcgagtgtgaccaagct
ttggtggggggcgttaatatgttactgtccccaaaaacgtttgccctgctttcacgcatg
catgcgctgtcacctggtggacgttgtaagactttctcggctgacgctgacgggtatgcc
cgcgccgaaggctgtgccgttgtcgtcctgaagcggctgtctgatgcacaacgggatcgc
gatccgatcctggcagtaatccgcggtacagcaattaaccatgatggtccgagcagtggc
ttgacagtgccctcgggtccggcacaggaagccttacttcgtcaagcgctggcacatgcg
ggcgtagtgcctgctgatgtggacttcgttgaatgccatggcacggggaccgctttaggt
gatccgattgaggttcgcgcactgtccgacgtatacggtcaggcccgcccggcggatcgt
ccgctcattctgggcgcggccaaagcgaatctcgggcacatggaaccggcagcaggctta
gctgggctgttgaaggccgtgctggcgctgggccaggaacaaattccggctcagcctgaa
ctgggtgaactgaacccgctgctgccatgggaagccctgcccgtggcggtggcacgtgcg
gcggtcccgtggccgcgcacggatcgtccgcgttttgcaggtgtgagttcgttcggtatg
agcggtaccaacgcgcatgttgtccttgaagaagcgcccgccgtagaattatggcctgcg
gcgccggaacgctcggcggaattgctggttctttctggcaagagcgagggcgcactggac
gcgcaggccgcacgcctgcgtgaacacttagacatgcatccggaactgggcctgggcgat
gtagccttctccctggcaacaacgcgcagcgcgatgaaccatcgtctggccgtggctgtg
acgagtcgcgaaggcttattagcagctctgagcgccgttgcgcagggtcaaaccccgccg
ggtgcggctcgttgcattgcgagctcaagccgtggtaagctggcctttctgttcactggc
cagggggcgcagaccccgggtatgggccgtgggctgtgcgcagcatggcctgctttccgc
gaagcatttgatcgctgcgtcgccttgtttgatcgcgaactggaccgcccgctgtgtgag
gttatgtgggccgagccgggttcggcggaatctctgttactcgatcaaacagcatttact
cagccagccctgtttacggtagaatatgccctgaccgcgctgtggagatcttggggcgtc
gaacctgaactggtggcggggcactcagcgggcgaactggtggcagcctgtgtagctggt
gtgttctctctggaagatggtgtccgccttgtcgcggcgcgtggccgcctgatgcagggt
ctgtccgctggtggcgcgatggttagtctgggtgctccggaggcggaagttgctgccgcc
gtagctccacatgcggcttgggtatcaatcgcagcggtaaatggtccggaacaagttgtc
attgcaggcgtggaacaggcagttcaggcaatcgcggcgggtttcgcagcacgcggggtc
cgtacgaaacggctgcacgttagtcatgctagccactctcctctgatggaacccatgctg
gaggagttcggccgcgttgctgcttctgttacctaccgccgcccatctgtgtcgctggtt
agcaacctgagtggtaaggttgtcaccgatgaactttctgccccgggttactgggtccgt
cacgtgcgtgaagcggtccgctttgcggatggtgtgaaagcgttacatgaggctggggct
ggtacgtttctggaggtagggcctaaaccgaccctcctgggccttctgccagcatgcctg
ccggaagcggagccgacgctgttggcgagccttcgcgcaggacgtgaggaagcagcaggc
gtcttagaggccctgggtcgtctttgggccgccggaggaagcgtctcgtggcccggtgtg
tttccgaccgctggccgccgtgtcccccttccaacctatccttggcaacgccagcgctac
tggctgcagatcgaacctgatagtcgtcgccacgcggcggcggatccgacacaaggttgg
ttttaccgcgtggattggccggaaattcctcggagtctccagaagtcagaggaggcttca
cgtgggagctggctggttctggccgataaaggcggtgtaggcgaagcggttgcggcggct
ctgtctacacgcgggttaccgtgcgttgtcctgcatgccccagccgaaacgtcagcgact
gcggagctggtgacggaggctgcgggcggtcgcagcgattggcaggttgtgctgtattta
tgggggcttgatgcggtcgtcggtgctgaagcaagtatcgatgaaattggggatgctact
cgtcgcgcgaccgccccggttctgggtctcgcgcgcttcctgtcgaccgttagttgtagc
cctcggctgtgggttgttacacgcggcgcgtgcatcgttggtgatgagcccgccatcgcg
ccgtgccaggcagcactgtgggggatgggtcgcgttgccgcacttgaacaccctggcgca
tgggggggcctcgtggatttggatccgcgagcgtctccgcctcaggcttcaccaatcgac
ggtgaaatgttagttactgaactgcttagtcaagaaaccgaagatcagcttgcgttccgc
cacggccgccgccatgccgctcgcctcgtagccgcgccaccgcgtggggaggcagcgcct
gcgtccttgagcgccgaagcaagttacctggtgaccggtggcctgggtggccttggcttg
attgtcgcgcagtggctggtggaattaggcgcccgtcatctcgtgctgacttcacgtcgc
gggttgccggatcgtcaggcttggcgcgaacagcaaccaccagaaatccgcgctcgtatc
gccgctgtggaagcactggaagctcgtggtgcccgcgttactgtagcagccgtggatgtc
gcagatgtcgaacctatgaccgccctcgtgtcttcagtggaaccgccgctgcgcggtgtt
gtccacgctgcgggcgtctcggttatgcgtccgctggctgaaacagatgagacgctgtta
gagtctgtgctgcgtcctaaggtggcggggagctggttattgcatcgcctgctgcacggc
cgtccgttggacctgtttgtgctgttctcaagcggtgccgccgtttggggcagtcacagc
cagggtgcgtatgctgctgcaaacgcgtttttggatggtctggcacatctgcgtcgctct
cagtcactgcccgccttaagcgtagcctggggtctctgggccgaaggtggcatggcggat
gctgaggcgcatgcccgcttatcagatattggtgtgcttccaatgtcgacctctgctgcc
ttatccgcattgcagcgtctggtggaaaccggcgcagcacaacgtactgtcacgcggatg
gactgggcccgctttgcgccagtgtacacggcacgtggccgtcgtaacctgctgagcgct
ttagtggctggtcgcgatattattgcgcctagccctccggcagctgctacacgtaattgg
cggggcctcagtgtcgcggaggcccgcatggcgctgcatgaagtggtccatggtgcagtt
gcgcgtgttttaggctttttggacccttctgcactggatccgggcatgggctttaacgaa
caaggtttggactctctgatggccgtggagattcggaaccttttgcaggcagaactggac
gtgcgtctctcaacgacattagcgttcgatcaccctactgtgcagcgcctggtggagcat
ctgctcgtggatgtgtctagtttagaagaccgctctgatacgcagcatgtgcgctcgctg
gcctccgacgagccaattgcaatcgtgggcgctgcctgccgttttccgggcggcgtggaa
gacctggaaagctactggcagttactggcagaaggggtagtggtttcggccgaagtccct
gcggaccgctgggacgcggccgattggtacgatccggatccggaaatcccagggcggacc
tatgttaccaaaggcgcgtttttgcgcgatcttcaacgcctggatgccacgttcttccgc
attagcccgcgtgaggctatgagcctcgacccgcaacagcgcctgcttttggaagtgtcc
tgggaagcgctggagagcgccggcatcgccccggacaccttgcgtgacagtccgactggt
gtcttcgtaggtgcgggcccaaacgagtattacacgcagcggttacggggttttactgac
ggcgccgctggtctctatggtggcactggcaacatgctctctgtggcagcagggcgcctt
tcgttttttttaggcttgcacgggccgacattggcgatggacacggcgtgttcgagctcg
ttagtagcgcttcatctggcttgtcagtcgctgcgtctgggtgaatgcgatcaggcattg
gttggcggcgtgaatgtccttttagcgccggaaacctttgtcctgctgtcacgtatgcgt
gccttgtcaccagatggtcgttgtaaaacattcagcgccgatgcagatggctacgcacgt
ggtgaaggctgtgcagtggtggttctgaaacgcctccgtgatgcgcagagggccggtgac
tcgattctggcgctgatccgcggtagtgctgtaaaccatgatggtccgtcctcgggtctg
accgtacctaatggtccggcgcaacaggcactcttgcgtcaggctctgagccaagcaggt
gtgtcccctgtggatgttgatttcgtcgaatgccatggcactggtacggctctgggtgac
ccgattgaagttcaagctctgagtgaagtatacggtccgggtcgtagcgaggatcgccct
ctcgtattaggcgccgttaaagccaatgttgcccacttggaagcagcgagcggcctggca
tcattactgaaagcggtgcttgcgttacgccacgaacagattccagcgcagccagagctc
ggggagctgaacccgcacttgccgtggaatactctcccagtggcggttccacgtaaagcc
gtgccatggggccgtggcgctcgtccgcgccgtgcgggcgtgagtgcctttggtttatcg
ggtaccaacgttcatgtggtgttagaagaagcgccggaggtagagttagtgccagctgca
cctgcgcgtccggtcgaactggtggtgttgagtgcgaaaagcgctgcggctctggacgct
gcggcagaacgcctgagcgcccatctgagcgcacatccggagctgtcgttgggcgatgta
gcctttagtctggctactactcggagcccgatggaacaccgcctggcgattgcgaccacc
agtcgcgaagccttacgtggtgccctggatgccgcagcccagcgccagaccccgcaaggc
gcagtgcgcggcaaagccgtatccagccgaggcaaattagccttcctgtttactggccag
ggggcccagatgccgggtatggggcgcggcctgtacgaagcttggcctgccttccgcgag
gcgtttgaccgctgcgtagcgctgtttgaccgtgaactggatcagccgttgcgtgaagtt
atgtgggcggcgccaggtttggcgcaagctgcgcgtttagatcaaactgcctacgcgcag
ccagccctgtttgcacttgaatacgcactggctgcgctgtggagatcttggggtgtcgaa
cctcacgttcttctgggtcattcgattggtgaactcgttgcggcgtgcgtggctggtgta
tttagcttagaggacgctgtgcgccttgtggccgcacgcgggcgtctgatgcaggcgttg
cccgctggtggcgccatggtggctatcgcagcgagtgaagcggaggtagcggcgagtgtc
gctccacacgcagccaccgtgagtatcgcagccgttaatggtccggatgccgtggtgatc
gcaggcgcggaagttcaggttctggcgttgggtgctaccttcgcggcgcgcgggatccgt
acgaaacgtctggccgtatctcacgcctttcattcaccgttgatggatcctatgctggag
gattttcaacgtgtcgcggcgaccattgcctatcgtgcaccggatcgtccggtagtgtcg
aacgttactggtcacgtggcaggtccggagatcgcgacacctgaatattgggttcgtcat
gtgcgtagcgcggttcgctttggcgatggtgctaaagcccttcacgctgcgggcgcagcg
acgtttgtagaaattgggccgaaacctgtattgctgggtctgctgccagcttgcctgggc
gaagcggacgcggtacttgtgccaagtttacgcgctgatcgctcagagtgcgaagtggtg
ctggcagcattaggcacatggtacgcctggggtggcgcactggactggaaaggcgtattt
ccggatggggcccgccgcgtcgcgctgccgatgtatccgtggcagcgcgaacgtcattgg
ctgcagctgacacctcgttctgcggctccagcgggcattgcgggtcgttggccgctggcg
ggcgtgggtctttgcatgccaggcgcggtgctccatcacgtgctgtcaatagggccacgt
catcagccattcctgggtgaccatctggtgtttggtaaagtcgtggtgccgggtgcattc
catgtggcggtgattctgagtatcgcagcggaacgctggcctgaacgtgcaatcgaactg
acaggcgttgaatttctgaaagccatcgctatggagccggatcaggaagtggaactgcat
gctgtcctgacgccggaggcggcaggggacgggtatctgttcgaactggcaaccttggcg
gcaccagaaactgagcgtcgttggacgacccatgctcgcggccgtgtgcaaccgacagat
ggggcaccgggggccttaccgcgtttagaggtgttagaagatcgcgccattcaacctttg
gactttgcgggcttcctggatcgcctctcagcagtccgcattggctggggcccgttgtgg
cggtggcttcaggatggtcgtgtgggtgacgaagctagcctggcgacgctggtgccgacc
tatccaaacgcccatgacgtggcgccgctgcacccgattttgttagataacggtttcgcg
gtgtcactgttggcgacccggtcggaaccagaagacgatggtactccaccgctgccgttt
gctgttgaacgcgtgcgctggtggcgtgcacctgttggtcgtgtccgctgtgggggcgtt
ccgcgctcacaggcattcggcgtctcttcgttcgtacttgtggacgaaactggtgaagtt
gtcgctgaggtggaaggctttgtgtgtcgccgcgctcctcgcgaagtctttctgcgtcag
gaatcaggggcgtctaccgctgccctgtatcgcctggattggcctgaggcgccgctgccg
gatgcgccagctgagcggatggaagaatcatgggtggtcgttgcagctccggggtccgaa
atggcagccgcactggctacgcgcctcaaccgctgcgtgctcgccgaacctaaaggtctg
gaggcggcactggcaggcgttagccctgccggtgtgatttgcctgtgggaacctggcgcg
catgaagaagcacctgcggcagcgcagcgtgtcgccacggaaggtctgtccgtcgtgcag
gcacttcgtgatcgcgccgtacgcctgtggtgggtaaccacaggggctgtggcggtggaa
gctggtgagcgcgtgcaggttgcaactgccccggtctgggggctcggccgcaccgtgatg
caagagcgtccggaactgtcttgtacgttagtggatctggaaccggaagtcgatgcagcc
cgtagcgccgacgttctgctccgggaattaggccgtgcggatgatgaaacgcaggtcgtc
ttccgttccggcgaacgccgtgtcgctcgcctggtcaaagcgaccacaccggaaggtctt
cttgtgccggacgccgaatcttatcgtctcgaagcaggtcagaaaggcaccctggatcag
ctgcggttggcaccagcccaacggcgggctccgggcccaggcgaagtggaaatcaaagta
accgcgagcggcctgaatttccgtactgttctcgctgttctggggatgtatcctggtgac
gcaggcccgatgggcggggattgtgccggcatcgtcaccgccgtgggccagggtgtccat
cacctgagcgtaggtgacgcggtgatgacgttaggcacattacaccgttttgtgacggtg
gatgctcggctggtggttcgtcaaccggctggcttgactcctgcccaagctgcgaccgtc
ccggttgcatttctgactgcgtggctggcactgcatgatctgggtaacctccgtcgtggt
gaacgcgtgctgattcatgccgccgcaggtggcgtcggcatggcggccgtccaaatcgca
cggtggatcggcgccgaagtttttgccaccgcctctccgtccaaatgggccgctgttcag
gcgatgggtgtgccgcgtacgcacattgccagttctaggactctggagttcgctgaaacc
ttccgccaagttacgggtggccgtggtgtcgatgttgtacttaatgctttggcgggcgag
tttgtggatgcatctctgagcctcttgaccactggtggtcgttttctggagatgggcaaa
acggacattcgcgatcgcgccgccgtcgctgccgcccacccaggggtgcgctaccgcgta
tttgacatcttagagctggcgccagatcggacccgtgagatcctggaacgcgtcgttgaa
ggtttcgcagcgggccatctccgcgctttgccggtgcatgcgtttgccattaccaaagcc
gaagcggcgttccgtttcatggcgcaggctcggcaccaaggcaaagtcgtcctgctccct
gcgccaagcgcggccccactggccccaacggggacggttctgctgaccggtggcttaggg
gcgctcgggttgcatgtggcacgctggttggctcagcagggcgctccacacatggtcctg
acgggtcgccgtggtttggataccccaggggcggccaaagcggttgccgaaattgaggct
cttggtgcgcgtgtcactattgccgcatctgatgtggctgatcgcaacgctctggaggcc
gttttacaagcaatcccagcggaatggccgctccaaggcgtgattcatgcggctggcgca
cttgatgatggtgtcctggatgaacagaccacggaccgtttcagccgtgtattagccccg
aaagtaactggcgcctggaacctgcacgagttaactgcggggaatgatctggcttttttt
gtgttgtttagctcaatgagtggtctgctcggttcagctggtcagtcgaactatgccgcc
gccaacacctttctggatgcgctggcggctcaccgccgcgcagaagggctggcagctcag
tcgctagcttggggtccgtggagtgatggcggtatggcggcgggtctttcagccgccctt
caagcacgtcttgcacgccacggtatgggcgccctttccccggcgcagggcaccgccctg
ctcggtcaagcgctggcacgcccggaaactcagctgggtgctatgtcccttgatgtgaga
gcggcctcccaggcgtccggcgccgcagttcctccagtttggcgtgccctggtgcgtgca
gaggctcgccatgccgccgcaggcgcccagggtgccttagcggcacgcctcggggctttg
cctgaagcccgccgcgcggacgaagtgcggaaagttgttcaagccgaaattgcacgcgtg
ctcagctggggggccgccagcgccgtacccgttgatcgcccgctgtctgatctgggttta
gattcacttacagctgtcgaattacgcaatgttctcggccagcgtgttggtgcaaccctg
ccagcgacccttgcgtttgatcacccaactgtagacgcactgacccgttggctcctggac
aaagtttctagtgtggcagaaccttccgtctccccagccaaaagctctccgcaggttgcg
ctcgatgaaccaattgcggttattgggatcggttgccgctttccgggtggtgttaccgat
ccggaaagcttctggcgcctgctggaagaaggtagcgatgcggtcgttgaggtcccgcat
gagcgctgggacatcgatgccttctatgacccagatccggatgtgcgtgggaaaatgact
acgcggtttggcgggtttttgtcggatattgaccgcttcgaacctgcatttttcggcatt
tccccgcgcgaagctacgaccatggatccgcagcagcgcctgctgctggaaacgagctgg
gaagcgtttgagcgtgccggcattctcccagagcgtcttatgggttcggatacgggtgtc
tttgtgggtcttttctatcaggaatatgcggccctggctggtggtattgaagcatttgac
ggttatctggggaccggcaccacggcatccgtcgcgagcggccgtatctcgtatgttctg
ggcttaaaaggtccgtcgttgactgttgatacggcgtgtagttcgtcgctggtggccgta
catctggcatgccaagcgctccggcggggcgaatgcagtgtcgccttagcaggtggggtg
gctttgatgttgaccccagctacatttgttgagttcagtcgtctgcgcggcttggcgccg
gacggtcgttgcaaatcattcagcgctgccgcagatggtgttggttggtccgaaggctgt
gcgatgctgctcctcaaaccgctgcgcgatgcccaacgcgacggcgatccgatcttagcg
gtgatccgcgggaccgccgtaaaccaagatggccgtagcaacggtttaacggcgcctaat
ggctccagccagcaggaagtcatccgtcgcgcattagagcaggcaggcttagcgccagcc
gacgtgagttatgtcgagtgtcatggtacgggaaccaccctcggtgatccgatcgaagtg
caggcgttgggtgccgtattagcacagggccgcccgagtgatcgtccgctggtaattggt
agcgtcaaaagcaacattgggcatacccaggctgcggcaggcgtggcgggtgtgatcaaa
gtagctctggctctcgaacggggcctgattccgcgctccttgcattttgatgccccgaac
ccgcacattccgtggtccgaactggccgtgcaggtcgcggccaaacctgtggagtggaca
cgcaacggcgcaccgcgtcgcgcaggcgtatcgagttttggtgtcagcggtaccaatgcc
cacgtcgtgttagaagaagccccagcagcggccttcgcaccggccgccgcccggtcagcc
gagttgtttgtgctgtcggcgaaatctgcggcggccctggatgcccaggcggcacgtctt
tctgcgcatgtcgttgcacatcctgaattgggcttaggcgatctggcctttagtctggcg
actacccgctcaccaatgacgtatcgcttagcagtagctgcgaccagccgcgaggcgttg
tctgcggccctggataccgccgcacaagggcaagcacctccagctgctgcgcgtggtcac
gcgagtactggctcggcgccgaaagttgtatttgtgttccctggccaagggagccaatgg
ttaggtatggggcagaaactgctgtccgaagaacctgtattccgtgacgctctgtcagct
tgcgatcgtgcgattcaagcggaggctgggtggtccttactggcagaactggcagcagat
gaaaccacctcacagttgggtcgcattgatgtggtgcagcctgcgctttttgccatcgaa
gtggcactgagcgcgctgtggagatcttggggtgtggaaccggatgccgtggttggtcat
tctatgggcgaagtggcggcggcccacgtagcaggcgcccttagtctggaagacgcggta
gcgatcatttgcaggcgcagccttttgctgcgccgtattagcgggcaaggcgaaatggca
gtggtcgaactgtccctggctgaagcggaagccgcgctgctgggttatgaagaccgtctt
agcgttgctgtttcgaactcgccacgctcaaccgtgcttgcgggcgagcccgctgcgctg
gccgaagttttagcgatcctggcagcaaaaggcgtcttctgtcgtcgcgtgaaagtagat
gtagctagccacagccctcagattgatccattacgtgacgaactgttagcggcgctgggc
gaactggaaccacgtcaggccacggtctctatgcggtccacagtaacaagcacgattgtg
gcgggcccggaactggtggcgagctattgggcagataatgtgcgccaacccgtccgcttc
gcggaagcggtgcaatctctcatggaaggcgggcatgggctgtttgtcgaaatgtcgccg
caccctattttgaccaccagcgtcgaagaaatccgtcgggctactaaacgtgaaggcgtt
gcggtagggtcgctgcgtcgcggccaagatgaacggttgtctatgctggaagcgctgggc
gcactgtgggtgcatgggcaggctgtaggttgggaacgcctgtttagtgcgggcggcgca
gggctgcgccgtgttccattaccaacgtacccgtggcagcgcgaacgctattggctgcag
gcaccaacaggtggtgcggcgagcggcagccgttttgcgcatgctgggtcgcatccgctg
ctgggtgaaatgcagacccttagtacccagcgtagcacccgcgtctgggagaccacactc
gatctgaaacggctgccgtggctgggtgatcaccgtgtacagggggctgtagttttcccg
ggtgctgcctatctggaaatggcgctgagttccggtgcggaggctctgggggatggtcct
ctccaggttagtgatgtggtcctggcggaagccctcgctttcgcggacgacaccccggtg
gctgtgcaggtaatggctacggaagagcgtccgggccgtttacaatttcatgtggcgtca
cgtgttccgggccacggccgcgctgcttttcgctctcacgcacgcggcgtccttcgtcag
accgagcgcgcagaggtgccagcacgcctggacctggccgcgctgcgcgcacgccttcag
gccagtgccccagctgccgccacctacgcagccctggccgaaatgggtttagaatacggc
cctgcctttcaaggtttagttgaactgtggcggggtgagggcgaggcgctgggtcgcgta
cgtcttccggaggccgctggcagcccggccgcttgtcgtctgcatccagcactgctggac
gcctgctttcacgtttcttctgcgtttgctgatcgcggggaggccacaccttgggtgccg
gtagaaatcggttctctgcgctggtttcagcggccgtcaggcgagctttggtgtcatgcc
cgtagcgtatcccatggcaaacctacgcctgatcgccgctcaacagacttttgggtggtt
gactcgactggcgcgatcgtggccgagatttccgggttggttgcacagcgtttggcaggc
ggcgttcgtcgccgggaagaggacgattggttcatggaacctgcttgggagccgacagct
gtgcctggctctgaagttactgcgggccgttggctgttgattgggtcgggtggtgggctg
ggtgcagccctgtatagtgctctgacggaagcaggccacagcgtggtccacgccaccggc
cacggcaccagcgcggcgggcttgcaggctctgctgacggcatcgtttgacggtcaggct
ccgactagcgtcgttcacctaggttcactggatgaacgcggtgttcttgatgccgacgca
ccgtttgatgctgacgccctggaagagtcgctggtgcgcggctgcgattccgtactgtgg
accgtccaggcggttgcaggtgcggggttccgtgatccgccacgtctttggttagtgacg
cgtggggcgcaggccattggcgccggtgatgtctctgtggcgcaagccccactgctgggt
ctcggccgtgtgatcgcattggagcacgccgaactgcgttgcgcccgcatcgacctggat
ccggcgcgtcgcgacggcgaagtcgatgagcttcttgcagagctgttggctgacgatgcc
gaggaagaagttgcgtttcgcggcggcgaacgccgggtggcccgcctcgtgcgtcgttta
ccggagacagattgtcgtgaaaaaatcgaaccagctgaaggccgcccttttcgtctggag
attgacggttcaggtgtcctggacgatttggttctgcgtgccacggaacgtcgtcctccg
ggcccgggggaagttgaaatcgccgtggaagccgccggcctgaattttttggatgtgatg
cgtgcaatgggcatttaccctggtccgggcgacggtccagtagcactgggcgccgaatgt
agtggtcgtattgttgctatgggcgaaggcgtcgaaagccttcggatcggccaagatgtc
gtcgcggtcgcacctttctcttttggtactcatgtgacaatcgatgcccgtatggtcgcc
ccgcgtccagcggcgctgaccgcagcgcaggcggctgccctgcctgtggccttcatgacg
gcatggtatggtttagtgcatctgggtcgtctgcgtgcgggcgaacgtgttttgattcat
agcgccactggcggcactggccttgcggcagtacaaatcgcgcgccatctcggggcggag
atatttgcgacagcaggcaccccggaaaaacgcgcatggctccgcgaacaaggtattgcg
catgtaatggattctaggtcattagactttgctgaacaggtcctggccgcgaccaaaggt
gaaggcgtggatgtggttttaaactccctgtccggtgcggcaatcgatgcttcattagcc
actttagttccagacggccgtttcatcgaactgggtaaaacggacatttacgccgatcgc
agcctggggctggcccacttccgcaaaagcctttcctacagcgcagtcgatctggctggt
ttagcggttcggcgcccggagcgtgttgcggctctgcttgctgaggtggtagacctgctg
gcacgtggtgcgcttcagccgttgccggtagaaatctttcctttgagccgcgcggccgac
gcgtttcgcaaaatggcacaagctcaacatctgggtaaattggtcctggcattagaggat
ccggatgtgcgcattcgcgtcccaggcgagagtggggtagcaattcgcgcagacggcacg
tacctggtgaccggtgggttaggtgggctgggtcttagcgtagcgggttggttggccgaa
cagggcgcgggccatctggttctggttggtcgctcgggtgccgtcagtgcagaacaacag
accgccgtagcggccctggaagcacacggggctcgcgttacagttgctcgtgccgacgtt
gcggatcgtgcacagatcgaacgtatccttcgcgaagtgaccgcgtcgggcatgccgctt
cgtggtgtggtgcatgcagctggcatcctggatgacggcctgctgatgcagcagaccccg
gcacgttttcgcgcagttatggctccgaaagtcagaggtgcccttcacttgcatgcgctg
acccgtgaagcgccactgagttttttcgtgttatatgcgagtggtgcgggccttttgggt
agtccagggcagggcaactatgccgccgcgaacactttcttagatgcattagcacaccac
cggcgcgcgcagggcctcccagccttaagtattgactggggtctgttcgctgatgtgggg
ttggccgctggacagcagaatcgcggcgcgcgcctggtaacacgtgggactcgcagtctg
accccggatgaaggtctgtgggcacttgaacgtctcctggatggcgatcggactcaggca
ggggtgatgccgttcgacgtgcgccaatgggtggagttctatccggccgctgcttcttca
cgtcgcctgagtcgcttggttaccgcccgccgtgtggcgagcggccgtctggcaggcgat
cgcgatctcttagagcgcctcgctacggcagaagcgggtgcccgtgcaggtatgctccag
gaagttgttcgcgcacaagtgtctcaagtgcttcgtctcccggaagggaaacttgacgtt
gacgctccgctgacctccctgggcatggatagcttgatgggtcttgaattgcgtaaccgc
attgaagctgttttggggatcaccatgcctgcgaccctgctgtggacttatcctaccgtc
gcggccctgagtgcgcacctggcgtcccatgtgtctagtactggtgatggcgagtctgcc
cgtccaccggacacaggtaatgttgcccctatgacccatgaagtggcgtcattagatgaa
gatgggttgtttgctctgatcgacgaatccctggcgcgcgcaggcaaacgcgggaattcg
agctccgtcgacaagcttgcggccgcactcgagcaccaccaccaccaccactga
5' End
3' End
NotesNo accession number for the natural gene was mentioned in the paper, so the record AF217189 was used. Two polypeptides were interacted with the help of linker pairs from stiB and stiC.
Expression VectorNAP(BAD)/Myc-HisA (Invitorgen)
Assay MethodsNASDS-PAGE (Coomassie blue staining)
ResultsNot determinedWhen the long size epoD was separated into two smaller polypeptides, both polypeptides were successfully expressed. (Fig. 3B, Lane 2).
Protein Functiontype I polyketide synthase
Recoding PurposeTo improve expression
Synthesized ByAuthors
Recoding MethodE. coli preferred codons were used for the whole gene. Designed by ralph Reid with DNAWorks. The
epoD was devided into two smaller proteins: module3-4 and module5-6. To facilitate cloning, amino
acid substitutions were introduced at I909L; E910Q; L1507S; 1508S; P2402L; 2403Q; L3006S; K3007S;
L5083S; 5084S; V5979L; E5980Q; V7212S
Publication Author(s)Mutka SC, Carney JR, Liu Y, Kennedy J.
Corresponding AuthorJonathan Kennedy
Corresponding AddressKosan Biosciences, Inc., 3832 Bay Center Place, Hayward, California 94545.
Publication Year2006
Publication TitleHeterologous Production of Epothilone C and D in Escherichia coli.
AbstractThe epothilones are a family of polyketide natural products that show a high potential as anticancer drugs. They are synthesized by the action of a hybrid nonribosomal peptide synthetase/polyketide synthase in the myxobacterium Sorangium cellulosum. In this work, the genes encoding the entire cluster,epoA, epoB, epoC, epoD, epoE, and epoF, were redesigned and synthesized to allow for expression in Escherichia coli. The expression of the largest of the proteins, EpoD, also required the protein be separated into two polypeptides with compatible module linkers. Using a combination of lowered temperature, chaperone coexpression, and alternative promoters, we succeeded in producing a soluble protein from all genes in the epothilone cluster. The entire synthetic epothilone cluster was then expressed in a strain of E. coli modified to enable polyketide biosynthesis, resulting in the production of epothilones C and D. Furthermore, feeding a thioester of the normal substrate for EpoD to cells expressing the epoD, epoE, and epoF genes also led to the production of epothilones C and D. The design of the synthetic epothilone genes together with E. coli expression provides the ideal platform for both the biochemical investigation of the epothilone PKS and the generation of novel biosynthetic epothilone analogues.
JournalBiochemistry. 2006 Jan 31;45(4):1321-30.. 45(4): 1321-30.
SummaryA cluster of epothilone genes were fully codon-optimized towards E. coli codon preference (with DNAWorks). It appeared that the synthetic epothilone genes were successfully expressed to a detectable level.
Comments
Discussion http://www.evolvingcode.net/forum/viewtopic.php?t=505
PubMed ID16430229
Submitter NameWu, Gang
Submitter AddressDepartment of Biological Sciences, University of Maryland Baltimore County, 1000 Hilltop Circle, Baltimore, MD 21250 USA
Entry ConfirmationNo
 
 

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