Synthetic Gene DataBase
 

Synthetic Gene 200


 
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Field NameNatural GeneSynthetic Gene
SGDB Gene ID186200
GenBank AccessionAF217189DQ289495
GenBank GI745355482880272
Gene NameepoEepoE(SYN)
Gene Length (bp)1139711457
SpeciesPolyangium cellulosum (Sorangium cellulosum)Escherichia coli
StrainsSMP44K207-3
CDSgtgacagaccgagaaggccagctcctggagcgcttgcgtgaggttactctggcccttcgc
aagacgctgaacgagcgcgataccctggagctcgagaagaccgagccgatcgccatcgtg
gggatcggctgccgcttccccggcggagcgggcactccggaggcgttctgggagctgctc
gacgacgggcgcgacgcgatccggccgctcgaggagcgctgggcgctcgtaggtgtcgac
ccaggcgacgacgtaccgcgctgggcggggctgctcaccgaagccatcgacggcttcgac
gccgcgttcttcggtatcgccccccgggaggcacggtcgctcgacccgcagcatcgcttg
ctgctggaggtcgcctgggaggggttcgaagacgccggcatcccgcctaggtccctcgtc
gggagccgcaccggcgtgttcgtcggcgtctgcgccacggagtatctccacgccgccgtc
gcgcaccagccgcgcgaagagcgggacgcgtacagcaccaccggcaacatgctcagcatc
gccgccggacggctatcgtacacgctggggctgcagggaccttgcctgaccgtcgacacg
gcgtgctcgtcatcgctggtggccattcacctcgcctgccgcagcctgcgcgctcgagag
agcgatctcgcgctggcgggaggggtcaacatgcttctctcccccgacacgatgcgagct
ctggcgcgcacccaggcgctgtcgcccaatggccgttgccagaccttcgacgcgtcggcc
aacgggttcgtccgtggggagggctgcggtctgatcgtgctcaagcgattgagcgacgcg
cggcgggatggggaccggatctgggcgctgatccgaggatcggccatcaatcaggacggc
cggtcgacggggttgacggcgcccaacgtgctcgcccagggggcgctcttgcgcgaggcg
ctgcggaacgccggcgtcgaggccgaggccatcggttacatcgagacccacggggcggcg
acctcgctgggcgaccccatcgagatcgaagcgctgcgcaccgtggtggggccggcgcga
gccgacggagcgcgctgcgtgctgggcgcggtgaagaccaacctcggccacctggagggc
gctgccggcgtggcgggcctgatcaaggctacactttcgctacatcacgagcgcatcccg
aggaacctcaactttcgtacgctcaatccgcggatccggatcgaggggaccgcgctcgcg
ttggcgaccgaaccggtgccctggccgcggacgggccggacgcgcttcgcgggagtgagc
tcgttcgggatgagcgggaccaacgcgcatgtggtgttggaggaggcgccggcggtggag
cctgaggccgcggcccccgagcgcgctgcggagctgttcgtcctgtcggcgaagagcgtg
gcggcgctggatgcgcaggcagcccggctgcgggaccacctggagaagcatgtcgagctt
ggcctcggcgatgtggcgttcagcctggcgacgacgcgcagcgcgatggagcaccggctg
gcggtggccgcgagctcgcgcgaggcgctgcgaggggcgctttcggccgcagcgcagggg
catacgccgccgggagccgtgcgtgggcgggcctccggcggcagcgcgccgaaggtggtc
ttcgtgtttcccggccagggctcgcagtgggtgggcatgggccgaaagctcatggccgaa
gagccggtcttccgggcggcgctggagggttgcgaccgggccatcgaggcggaagcgggc
tggtcgctgctcggggagctctccgccgacgaggccgcctcgcagctcgggcgcatcgac
gtggttcagccggtgctcttcgccatggaagtagcgctttctgcgctgtggcggtcgtgg
ggagtggagccggaagcggtggtgggccacagcatgggcgaggtggcggcggcgcacgtg
gccggcgcgctgtcgctcgaggacgcggtggcgatcatctgccggcgcagccggctgctg
cggcggatcagcggtcagggcgagatggcgctggtcgagctgtcgctggaggaggccgag
gcggcgctgcgtggccatgagggtcggctgagcgtggcggtgagcaacagcccgcgctcg
accgtgctcgcaggcgagccggcggcgctctcggaggtgctggcggcgctgacggccaag
ggggtgttctggcggcaggtgaaggtggacgtcgccagccatagcccgcaggtcgacccg
ctgcgcgaagagctgatcgcggcgctgggggcgatccggccgcgagcggctgcggtgccg
atgcgctcgacggtgacgggcggggtgatcgcgggtccggagctcggtgcgagctactgg
gcggacaatcttcggcagccggtgcgcttcgctgcggcggcgcaagcgctgctggaaggt
ggccccacgctgttcatcgagatgagcccgcacccgatcctggtgccgcccctggacgag
atccagacggcggtcgagcaagggggcgctgcggtgggctcgctgcggcgagggcaggac
gagcgcgcgacgctgctggaggcgctggggacgctgtgggcgtccggctatccggtgagc
tgggctcggctgttccccgcgggcggcaggcgggttccgctgccgacctatccctggcag
cacgagcggtgctggatcgaggtcgagcctgacgcccgccgcctcgccgcagccgacccc
accaaggactggttctaccggacggactggcccgaggtgccccgcgccgccccgaaatcg
gagacagctcatgggagctggctgctgttggccgacaggggtggggtcggcgaggcggtc
gctgcagcgctgtcgacgcgcggactttcctgcaccgtgcttcatgcgtcggctgacgcc
tccaccgtcgccgagcaggtatccgaagctgccagtcgccgaaacgactggcagggagtc
ctctacctgtggggcctcgacgccgtcgtcgatgctggggcatcggccgacgaagtcagc
gaggctacccgccgtgccaccgcacccgtccttgggctggttcgattcctgagcgctgcg
ccccatcctcctcgcttctgggtggtgacccgcggggcatgcacggtgggcggcgagcca
gaggtctctctttgccaagcggcgttgtggggcctcgcgcgcgtcgtggcgctggagcat
cccgctgcctggggtggcctcgtggacctggatcctcagaagagcccgacggagatcgag
cccctggtggccgagctgctttcgccggacgccgaggatcaactggcgttccgcagcggt
cgccggcacgcagcacgccttgtagccgccccgccggagggcgacgtcgcaccgatatcg
ctgtccgcggagggaagctacctggtgacgggtgggctgggtggccttggtctgctcgtg
gctcggtggctggtggagcggggagctcgacatctggtgctcaccagccggcacgggctg
ccagagcgacaggcgtcgggcggagagcagccgccggaggcccgcgcgcgcatcgcagcg
gtcgaggggctggaagcgcagggcgcgcgggtgaccgtggcagcggtggatgtcgccgag
gccgatcccatgacggcgctgctggccgccatcgagcccccgttgcgcggggtggtgcac
gccgccggcgtcttccccgtgcgtcccctggcggagacggacgaggccctgctggagtcg
gtgctccgtcccaaggtggccgggagctggctgctgcaccggctgctgcgcgaccggcct
ctcgacctgttcgtgctgttctcgtcgggcgcggcggtgtggggtggcaaaggccaaggc
gcatacgccgcggccaatgcgttcctcgacgggctcgcgcaccatcgccgcgcgcactcc
ctgccggcgttgagcctcgcctggggcctatgggccgagggaggcgtggttgatgcaaag
gctcatgcacgtctgagcgacatcggagtcctgcccatggccacggggccggccttgtcg
gcgctggagcgcctggtgaacaccagcgctgtccagcgttcggtcacacggatggactgg
gcgcgcttcgcgccggtctatgccgcgcgagggcggcgcaacttgctttcggctctggtc
gcggaggacgagcgcactgcgtctcccccggtgccgacggcaaaccggatctggcgcggc
ctgtccgttgcggagagccgctcagccctctacgagctcgttcgcggcatcgtcgcccgg
gtgctgggcttctccgacccgggcgcgctcgacgtcggccgaggcttcgccgagcagggg
ctcgactccctgatggctctggagatccgtaaccgccttcagcgcgagctgggcgaacgg
ctgtcggcgactctggccttcgaccacccgacggtggagcggctggtggcgcatctcctc
accgacgtgctgaagctggaggaccggagcgacacccggcacatccggtcggtggcggcg
gatgacgacatcgccatcgtcggtgccgcctgccggttcccgggcggggatgagggcctg
gagacatactggcggcatctggccgagggcatggtggtcagcaccgaggtgccagccgac
cggtggcgcgcggcggactggtacgaccccgatccggaggttccgggccggacctatgtg
gccaagggggccttcctccgcgatgtgcgcagcttggatgcggcgttcttctccatctcc
cctcgtgaggcgatgagcctggacccgcaacagcggctgttgctggaggtgagctgggag
gcgatcgagcgcgctggccaggacccgatggcgctgcgcgagagcgccacgggcgtgttc
gtgggcatgatcgggagcgagcacgccgagcgggtgcagggcctcgacgacgacgcggcg
ttgctgtacggcaccaccggcaacctgctcagcgtcgccgctggacggctgtcgttcttc
ctgggtctgcacggcccgacgatgacggtggacaccgcgtgctcgtcgtcgctggtggcg
ttgcacctcgcctgccagagcctgcgattgggcgagtgcgaccaggcactggccggcggg
tccagcgtgcttttgtcgccgcggtcattcgtcgcggcatcgcgcatgcgtttgctttcg
ccagatgggcggtgcaagacgttctcggccgctgcagacggctttgcgcgggccgagggc
tgcgccgtggtggtgctcaagcggctccgtgacgcgcagcgcgaccgcgaccccatcctg
gcggtggtccggagcacggcgatcaaccacgatggcccgagcagcgggctcacggtgccc
agcggtcctgcccagcaggcgttgctaggccaggcgctggcgcaagcgggcgtggcaccg
gccgaggtcgatttcgtggagtgccacgggacggggacagcgctgggtgacccgatcgag
gtgcaggcgctgggcgcggtgtatggccggggccgccccgcggagcggccgctctggctg
ggcgctgtcaaggccaacctcggccacctggaggccgcggcgggcttggccggcgtgctc
aaggtgctcttggcgctggagcacgagcagattccggctcaaccggagctcgacgagctc
aacccgcacatcccgtgggcagagctgccagtggccgttgtccgcgcggcggtcccctgg
ccgcgcggcgcgcgcccgcgtcgtgcaggcgtgagcgctttcggcctgagcgggaccaac
gcgcatgtggtgttggaggaggcgccggcggtggagcctgaggccgcggcccccgagcgc
gctgcggagctgttcgtcctgtcggcgaagagcgtggcggcgctggatgcgcaggcagcc
cggctgcgggatcatctggagaagcatgtcgagcttggcctcggcgatgtggcgttcagc
ctggcgacgacgcgcagcgcgatggagcaccggctggcggtggccgcgagctcgcgcgag
gcgctgcgaggggcgctttcggccgcagcgcaggggcatacgccgccgggagccgtgcgt
gggcgggcctccggcggcagcgcgccgaaggtggtcttcgtgtttcccggccagggctcg
cagtgggtgggcatgggccgaaagctcatggccgaagagccggtcttccgggcggcgctg
gagggttgcgaccgggccatcgaggcggaagcgggctggtcgctgctcggggagctctcc
gccgacgaggccgcctcgcagctcgggcgcatcgacgtggttcagccggtgctcttcgcc
gtggaagtagcgctttcagcgctgtggcggtcgtggggagtggagccggaagcggtggtg
ggccacagcatgggcgaggttgcggcggcgcacgtggccggcgcgctgtcgctcgaggat
gcggtggcgatcatctgccggcgcagccggctgctgcggcggatcagcggtcagggcgag
atggcgctggtcgagctgtcgctggaggaggccgaggcggcgctgcgtggccatgagggt
cggctgagcgtggcggtgagcaacagcccgcgctcgaccgtgctcgcaggcgagccggcg
gcgctctcggaggtgctggcggcgctgacggccaagggggtgttctggcggcaggtgaag
gtggacgtcgccagccatagcccgcaggtcgacccgctgcgcgaagagctggtcgcggcg
ctgggagcgatccggccgcgagcggctgcggtgccgatgcgctcgacggtgacgggcggg
gtgattgcgggtccggagctcggtgcgagctactgggcggacaatcttcggcagccggtg
cgcttcgctgcggcggcgcaagcgctgctggaaggtggccccacgctgttcatcgagatg
agcccgcacccgatcctggtgccgcctctggacgagatccagacggcggtcgagcaaggg
ggcgctgcggtgggctcgctgcggcgagggcaggacgagcgcgcgacgctgctggaggcg
ctggggacgctgtgggcgtccggctatccggtgagctgggctcggctgttccccgcgggc
ggcaggcgggttccgctgccgacctatccctggcagcacgagcggtactggatcgaggac
agcgtgcatgggtcgaagccctcgctgcggcttcggcagcttcataacggcgccacggac
catccgctgctcggggctccattgctcgtctcggcgcgacccggagctcacttgtgggag
caagcgctgagcgacgagaggctatcctatctttcggaacatagggtccatggcgaagcc
gtgttgcccagcgcggcgtatgtagagatggcgctcgccgccggcgtagatctctatggc
gcggcgacgctggtgctggagcagctggcgctcgagcgagccctcgccgtgccttccgaa
ggcggacgcatcgtgcaagtggccctcagcgaagaagggcccggtcgggcctcattccag
gtatcgagccgtgaggaggcaggtagaagctgggttcggcacgccacggggcacgtgtgt
agcgaccagagctcagcagtgggagcgttgaaggaagctccgtgggagattcaacagcga
tgtccgagcgtcctgtcgtcggaggcgctctatccgctgctcaacgagcacgccctcgac
tatggcccctgcttccagggtgtggagcaggtgtggctcggcacgggggaggtgctcggc
cgggtacgcttgccagaagacatggcatcctcaagtggcgcctatcggattcatcccgcc
ttgttggatgcatgttttcaagtgctgaccgcgctgctcaccacgccggaatccatcgag
attcggaggcggctgacggatctccacgaaccggatctcccgcggtccagggctccggtg
aatcaagcggtgagtgacacctggctgtgggacgccgcgctggacggtggacggcgccag
agcgcgagcgtgcccgtcgacctggtgctcggcagcttccacgcgaagtgggaggtcatg
gatcgcctcgcgcagacgtacatcatccgcactctccgcacatggaacgtcttctgcgct
gctggagagcgtcacacgatagacgagttgctcgtcaggctccaaatctctgctgtctac
aggaaggtcatcaagcgatggatggatcaccttgtcgcgatcggcgtccttgtaggggac
ggagagcatcttgtgagctctcagccgctgccggagcatgattgggcggcggtgctcgag
gaggccgcgacggtgttcgccgacctcccagtcctacttgagtggtgcaagtttgccggg
gaacggctcgcggacgtgttgaccgggaagacgctggcgctcgagatcctcttccctggc
ggctcgttcgatatggcggagcgaatctatcaagattcgcccatcgcccgttactcgaac
ggcatcgtgcgcggtgtcgtcgagtcggcggcgcgggtggtagcaccgtcgggaacgttc
agcatcttggagatcggagcagggacgggcgcgaccaccgccgccgtcctcccggtgttg
ctgcctgaccggacagaataccatttcaccgatgtttctccgctcttccttgctcgtgcg
gagcaaagatttcgagatcatccattcctgaagtatggtattctggatatcgaccaggag
ccagctggccagggatacgcacatcagaagttcgacgtcatcgtcgcggccaacgtcatc
catgcgacccgcgatataagagccacggcgaagcgtctcctgtcgttgctcgcgcccgga
ggccttctggtgctggtcgagggcacagggcatccgatctggttcgatatcaccacggga
ttgatcgaggggtggcagaagtacgaagatgatcttcgtaccgaccatccgctcctgcct
gctcggacctggtgtgacgtcctgcgccgggtaggctttgcggatgccgtgagtctgcca
ggcgacggatctccggcggggatcctcggacagcacgtgatcctctcgcgcgctccgggc
atagcaggagccgcttgtgacagctccggtgagtcggcgaccgaatcgccggccgcgcgt
gcagtacggcaggaatgggccgatggctccgctgacggcgtccatcggatggcgttggag
agaatgtacttccaccgccggccgggccggcaggtttgggtccacggtcgattgcgtacc
ggtggaggcgcgttcacgaaggcgctcactggagatctgctcctgttcgaagagaccggg
caggtcgtggcagaggttcaggggctccgcctgccgcagctcgaggcttctgctttcgcg
ccgcgggacccgcgggaagagtggttgtacgcgttggaatggcagcgcaaagaccctata
ccagaggctccggcagccgcgtcttcttccaccgcgggggcttggctcgtgctgatggac
cagggcgggacaggcgctgcgctcgtatcgctgctggaagggcgaggcgaggcgtgcgtg
cgcgtcgtcgcgggtacggcatacgcctgcctcgcgccggggctgtatcaagtcgatccg
gcgcagccagatggctttcataccctgctccgcgatgcattcggcgaggaccggatgtgc
cgcgcggtagtgcatatgtggagccttgatgcgaaggcagcaggggagaggacgacagcg
gagtcgcttcaggccgatcaactcctggggagcctgagcgcgctttctctggtgcaggcg
ctggtgcgccggaggtggcgcaacatgccgcgactttggctcttgacccgcgccgtgcat
gcggtgggcgcggaggacgcagcggcctcggtggcgcaggcgccggtgtggggcctcggt
cggacgctcgcgctcgagcatccagagctgcggtgcacgctcgtggacgtgaacccggcg
ccgtctccagaggacgcagctgcactcgcggtggagctcggggcgagcgacagagaggac
cagatcgcattgcgctcgaatggccgctacgtggcgcgcctcgtgcggagctccttttcc
ggcaagcctgctacggattgcggcatccgggcggacggcagttatgtgatcaccgatggc
atggggagagtggggctctcggtcgcgcaatggatggtgatgcagggggcccgccatgtg
gtgctcgtggatcgcggcggcgcttccgacgcctcccgggatgccctccggtccatggcc
gaggctggcgcagaggtgcagatcgtggaggccgacgtggctcggcgcgtcgatgtcgct
cggcttctctcgaagatcgaaccgtcgatgccgccgcttcgggggatcgtgtacgtggac
gggaccttccagggcgactcctcgatgctggagctggatgcccatcgcttcaaggagtgg
atgtatcccaaggtgctcggagcgtggaacctgcacgcgctgaccagggatagatcgctg
gacttcttcgtcctgtactcctcgggcacctcgcttctgggcttgcccggacaggggagc
cgcgccgccggtgacgccttcttggacgccatcgcgcatcaccggtgtaggctgggcctc
acagcgatgagcatcaactggggattgctctccgaagcatcatcgccggcgaccccgaac
gacggcggcgcacggctccaataccgggggatggaaggtctcacgctggagcagggagcg
gaggcgctcgggcgcttgctcgcacaacccagggcgcaggtaggggtaatgcggctgaat
ctgcgccagtggctggagttctatcccaacgcggcccgactggcgctgtgggcggagttg
ctgaaggagcgtgaccgcaccgaccggagcgcgtcgaacgcatcgaacctgcgcgaggcg
ctgcagagcgccaggcccgaagatcgtcagttggttctggagaagcacttgagcgagctg
ttggggcgggggctgcgccttccgccggagaggatcgagcggcacgtgccgttcagcaat
ctcggcatggactcgttgataggcctggagctccgcaaccgcatcgaggccgcgctcggc
atcaccgtgccggcgaccctgctatggacttaccctaccgtagcagctctgagcgggaac
ctgctagatattctgttcccgaatgccggcgcgactcacgctccggccaccgagcgggag
aagagcttcgagaacgatgccgcagatctcgaggctctgcggggtatgacggacgagcag
aaggacgcgttgctcgccgaaaagctggcgcagctcgcgcagatcgttggtgagtaa
atgaccgaccgtgaaggccagcttttggaacgcctgcgtgaagtgacgttggccctgcgg
aaaactctgaacgagcgcgataccttagagttagaaaaaacggaaccaattgccattgtc
ggcattggctgccgttttccaggcggtgcggggactccggaagctttttgggagctgctg
gatgatggtcgtgatgcgatccggccacttgaggagcggtgggcgctggtcggggtcgat
cctggtgatgacgtcccacgctgggctggccttctgactgaagcgattgacggctttgac
gcggccttctttggcattgcgccgcgcgaagcccgctctctcgatcctcagcaccggctg
ctgctggaagttgcatgggaagggtttgaagacgccggcatcccgccgcgtagcctggtc
gggagtcgcacgggtgtcttcgtaggcgtatgtgcaacagaatatttacatgcggcggtg
gctcaccagccgcgcgaggaacgcgatgcttatagcacaacgggtaacatgttgtctatt
gccgctggccgcttgtcatacacgcttggccttcagggcccttgcttgacagttgacaca
gcctgctcttcgagtctggtggcgatccacctggcgtgtcgctcactccgtgcgcgtgaa
tccgacttagcgctggcgggtggcgtcaatatgctgttatctcctgacaccatgcgcgcc
cttgctcgtacccaggcattgtccccgaacggtcgttgtcaaaccttcgatgcaagcgcg
aacggttttgtccggggcgagggttgtggcctgatcgtgcttaaacgtctctccgatgcg
cgtcgggacggcgaccgtatttgggccctgatccgcggcagcgctattaaccaggatggt
cgctccacaggtctgaccgcaccgaatgtactggctcagggcgcactgctgcgtgaagct
ttacgtaatgcaggggtggaagccgaagctattggctacatcgagactcatggcgccgcg
acttctttaggggatccgattgagatcgaagccctgcgcactgtggtgggcccggcgcgc
gctgatggcgcccgttgcgtgctcggcgcggtgaaaaccaacctgggccatttggaaggc
gcggccggggttgctgggctgatcaaagcaaccctgtctttgcaccatgaacgtattccg
cgcaacctgaatttccgtacacttaatccgcgtatccgcattgaagggacggcattagcc
ctcgctaccgaaccagttccatggcctcgcaccggccgtacgcggttcgccggtgtttca
agctttggcatgtcgggtaccaatgcgcatgttgttctggaggaagcccctgctgttgag
ccggaggcagcagcgccggaacgggctgccgagctgtttgtgttaagtgcgaaatcagtt
gccgccctggatgcccaagcagcgcgcctgcgtgatcacctggaaaaacatgtggaactg
ggtcttggtgacgtggcatttagcctggcgactacccgtagcgcaatggaacatcgcctg
gccgtggcagcgagctctcgtgaggcgctgcgcggggccctgtcggctgccgcccaaggc
cacacgccgccgggcgcggtgcggggccgcgcatccggtgggtcagcgccaaaagtggtc
ttcgtgttccctggccagggttcccagtgggtagggatgggccgtaaactgatggcggaa
gaacctgtctttcgcgcagcgctggagggctgcgaccgtgccatcgaagcagaagccggt
tggtccctgttaggtgagctgtcggcagatgaagccgcaagccagcttggccgtatcgac
gttgtccagccggtactgtttgctatggaagtggccttatcggccctgtggagatcttgg
ggtgtggagccagaggccgtagtgggtcactcaatgggcgaggtagccgctgcgcatgtg
gcaggtgccctgtctctggaagacgcggtggctattatttgccgtcgctcacgcctgctc
cgtcggatctcggggcaaggtgaaatggcactcgtggagctgtccctggaggaagccgaa
gcagccctgcgcggccatgaaggtcgcctgtctgttgctgtgtccaatagcccacgcagc
accgtactggccggtgaaccggccgcactgtcggaagttctggcagcgttgaccgcgaaa
ggcgttttctggcgtcaagttaaagtcgatgtggctagccactcgccgcaggtggacccg
ttgcgtgaagaactcattgccgccctgggtgccatccgcccacgcgcagccgctgttcca
atgcgttccaccgtgaccggcggtgttattgcaggcccggaactgggcgcgtcttattgg
gctgataacttgcgccaacccgtacggtttgcggctgccgcgcaagcactgctggaaggt
ggtccgacgctgttcatcgaaatgagtccgcatccgatccttgtcccgccgttggatgaa
attcagacggcggtcgaacaaggtggtgcagcggttgggtcactgcgccgtggtcaggac
gagcgtgcaactttactggaagcactggggaccctctgggcctcgggctacccggtatcg
tgggctcgtctgtttccagcggggggtcgtcgcgtaccgcttccaacgtatccgtggcaa
cacgagcgttgttggctgcaggttgaaccagatgctcgtcgtttagctgctgccgaccca
acgaaagattggttctatcgcactgactggccggaagttcctcgcgccgccccgaaaagt
gaaacagcacacgggagctggcttctcctcgctgaccgtggcggcgttggtgaggcggtc
gctgcggcacttagcacccgtggcctgagttgtaccgtgttacatgcgtccgctgatgca
tcgacggttgcggagcaagtgagcgaagccgccagccgtcgcaacgattggcagggggta
ttgtatctctggggtctggatgctgtcgttgatgctggcgcgagtgcagatgaagtttcg
gaagcgacacgccgcgcaaccgcgccggtgttaggtttggtgcgcttcctgtcagctgcg
ccgcatcctccccggttttgggttgtgaccagaggtgcgtgcaccgttggcggggagcct
gaagttagtctgtgccaggccgcgttgtggggtctggcacgtgtggtagcgcttgaacat
ccggcggcctggggtggcctggtcgatctggatccgcagaaatcaccgaccgaaattgaa
ccactggtggctgagctgctgagccctgatgccgaagaccagttggcttttcgtagtggc
cgtcgtcacgcagcgcggcttgtcgcagcgccgccggaaggtgatgtcgcgccgatcagt
cttagtgcggaaggctcttacttagtcaccggtggcttgggtggtctgggtcttctggtg
gcgcgctggttggtagagcgtggggcccgccacttggttctgacttcccgccatggcctg
cctgaacgtcaagcatcgggtggtgaacagccgccggaagcccgcgcacgcattgccgcc
gtggaaggtctggaagctcagggggcacgtgttaccgtagcggcggtggacgtagctgag
gcggaccctatgacggccttgttagctgctattgagcctccattgcgcggtgtcgttcac
gccgcaggtgtgtttccggtccgtccgctggctgaaactgatgaggccctcttagaaagc
gtattacgccctaaagttgccggtagttggttactgcatcggcttctgcgtgaccgtcct
ctggatttgtttgtactcttcagcagcggggcggcagtctgggggggcaaaggccagggc
gcgtatgcagcagcaaatgcgttcctggatggcttggcacatcatcgtcgcgcacattct
ctgccagccttaagtctcgcatggggcctgtgggcggagggcggcgtggttgatgccaaa
gcgcatgcgcgcttatctgacatcggcgttctcccaatggcgacgggcccggctctcagc
gcgctcgaacgcttagtgaacacaagtgcggtgcagcgcagcgtcacacgcatggattgg
gcccgctttgccccagtctacgccgctcgtggtcggcgtaacctgctttccgcgctggtt
gcggaagatgagcgcacggcaagccctccggttccaaccgcgaatcgcatttggcgcggt
ctgagcgtagcggaatcacgctcggcgctgtatgaactggtgcgtggtattgttgcacgg
gtgctgggcttctccgatccgggggcgctggacgtgggtcgcggcttcgcggagcagggc
ctggattcacttatggcgttggaaatccgcaatcgcttacagcgtgaactgggtgagcgt
ttaagcgccaccttagcttttgatcatccgacggtggaacgccttgtcgcgcacctgttg
actgatgtgtctagtcttgaagaccgttccgatacgcgccatatccgcagcgtggccgcc
gatgacgacatcgcaattgtgggcgccgcatgtcgttttccggggggcgatgaggggctg
gagacctactggcgtcacttagctgagggcatggtcgtttcaaccgaggtgccagcagac
cgttggcgcgctgcggactggtatgatccggatccggaagtaccaggtcgtacctacgtc
gcgaaaggtgccttcctccgtgacgtgcgttcgttagatgcggcatttttttccatcagt
ccgcgtgaagctatgagtttggatccgcagcagcgcctgctgctggaggtctcatgggaa
gctatcgagcgcgccggccaggacccgatggccttacgcgagagcgccactggcgtcttt
gtcggtatgatcggtagtgaacacgccgaacgggtccaaggtttagatgacgatgccgca
ctgctgtacggcaccaccgggaatttgctgtctgtggcagcaggccgcctgagttttttc
ctgggcctgcatggcccgacgatgaccgtggataccgcttgctctagctccctggtcgcc
ctgcacctggcttgccagtcattacgcctgggcgaatgcgatcaggcgctggctggcggt
tcctctgttctgctttcgcctcgctcatttgtggcggcctcccgtatgcgtttgctgagc
cctgatggtcgctgtaaaacgttcagcgcagccgccgatgggtttgcgcgtgccgaaggt
tgcgccgtggtggtattaaaacgcctgcgtgatgcccaacgtgaccgcgacccgattttg
gcggtggtaagatctacagccattaaccacgatgggcctagcagtggtctcaccgtcccg
tctgggccagcccaacaggcactgttgggtcaagctcttgctcaagcaggggtagcgcct
gccgaagttgactttgttgagtgtcacggaaccgggaccgcgctgggtgatccaatagag
gtccaggctttgggcgcagtgtatggccgtggtcgcccggcggagcgcccactgtggtta
ggggcagtgaaagcgaatcttgggcatctggaggcagccgctggcttggcaggcgttctg
aaagtgctgctggcattagaacatgaacaaattcctgcgcaaccggaactggatgagctg
aaccctcatattccatgggcggaactgccggttgcggttgtccgcgccgcagtgccgtgg
cctcgtggcgcacggccacgtcgcgccggtgtgtcggcattcggtctcagcggtaccaac
gctcacgtcgtgcttgaggaggcacctgctgttgaaccggaggcagccgcaccagaacgt
gcggccgaactgttcgttctgagcgctaaaagtgtggccgcgctggatgctcaggccgcc
cgcctgcgtgatcatctggaaaaacacgtggaacttgggctgggcgatgtcgctttctca
ttggctaccacacgttctgccatggagcatcgtctggcggttgcagccagctctcgtgaa
gccctgcgtggtgcgttgagtgccgccgcgcagggtcacactccgccgggtgccgttcgc
ggccgtgcttctggtggcagcgccccaaaagtagtgttcgttttccctggccagggttcg
cagtgggtaggcatgggccgtaaactgatggcggaggagcctgtatttcgtgccgccctt
gaaggctgcgatcgtgccatcgaagccgaagcaggctggtccctgcttggggaactcagt
gcggatgaagccgcctctcaacttggccgcattgatgtggtccagccggttctgtttgcg
gttgaagtggccctgtctgctctgtggagatcttggggcgttgaaccggaagctgttgta
ggtcatagcatgggcgaagtcgcagcagcccatgttgctggtgccttgtctctggaggat
gcggtggcgattatctgtcgtcgctctcgcctgctgcgccggatttcaggccaaggtgaa
atggccttagtggaactgtcgttagaggaagcggaagcagcattgcgcgggcatgaaggt
cgtctgagcgtggcagtctcaaactcgcctcgttctaccgttttagcaggtgaacctgct
gctttaagtgaagttctggccgcgttgaccgccaaaggtgtcttctggcgtcaagtgaaa
gtggatgttgctagccacagtccgcaagtggaccctttgcgcgaggagctggtagctgca
ttaggcgccatccgcccgcgcgctgcggcggtgccaatgcgcagcaccgtgaccgggggt
gtcattgcgggtcctgaactcggtgcgtcttattgggctgataacttgcgccagccagtc
cggtttgccgcagctgcacaagctttgttagaaggcgggccgactctcttcattgaaatg
tccccgcatccgatcctggttccgcctctcgatgaaatccagacagctgtggaacaaggg
ggtgcagcggttggttcactgcggcgtggtcaagatgaacgcgccacgctgctcgaagcc
ttgggcactctgtgggcgtcgggctatccggtgtcatgggcacgtctgtttcctgctggg
ggccgtcgtgtgcctctgccgacatacccgtggcagcatgagcggtactggctgcaggat
tctgtacatggcagcaaaccgtcccttcgcctgcgccaactccacaatggtgcaacggat
catccgttactgggtgcgccgttactggtcagcgcgcgccctggtgcacacctgtgggaa
caggctttgagcgacgaacgtctgtcttacctgtcagagcaccgtgtgcacggcgaagcg
gtgcttccaagcgctgcgtatgttgagatggcccttgccgcaggcgtcgacttgtatggc
gcggcgactttagtcttagagcagttggcattggaacgcgccctggcagtgcctagcgag
gggggccgcattgtacaggttgctctgtctgaagaaggcccgggccgtgcgtcttttcag
gtctcgtcccgtgaggaagccggtcgttcttgggtacgtcatgcgactgggcacgtatgc
agcgatcagtccagtgcggttggtgcgcttaaggaggcgccgtgggagattcaacagcgt
tgtccttccgttctgagctcggaagctctgtacccgttactgaacgaacatgctcttgac
tatgggccgtgttttcagggcgtagaacaggtttggctgggcactggcgaggtactgggg
cgcgtccgtctcccggaagacatggcttcgtccagcggtgcgtaccggatccatccggcc
ttgttagacgcgtgctttcaagtcctgaccgcactgcttacaacgccagaaagtatcgaa
atccgccgtcgcctgaccgatctgcacgagccagacctgccgcgtagccgtgcgccagta
aatcaggcagtgagcgatacctggctgtgggatgcagcattggatggtggtcgcagacag
tctgcctctgtacccgttgacttggtacttggttcttttcacgctaaatgggaagtaatg
gaccgtttggcgcaaacttatatcattcggacgcttcgcacatggaacgtcttttgcgcc
gccggcgaacgtcacactatcgacgagttattggtgcgtttacagattagtgcggtgtat
cgcaaagttattaaacgctggatggaccatctggtcgccattggcgtgctggtgggcgat
ggcgaacatctcgtatcatcgcagccactgccggaacacgactgggcggccgttttggag
gaggcggccaccgtgtttgcggacttaccagttttactggagtggtgtaaattcgcaggt
gaacgcctggctgatgtgctgaccggcaaaaccctggcgttggaaattctgtttccgggc
ggtagcttcgacatggcagaacgtatttatcaggactcccctattgcgcgttatagtaac
ggtatcgtccgtggtgtggtcgaatccgcagcccgcgtcgtggcgccttcgggcaccttt
tctatcttagaaattggcgcaggtacaggggcaacgacagcggccgttctgcctgttctg
ctgccggaccgtacggagtatcacttcaccgatgtatcgccgctgttcttagctcgtgcg
gaacaacgctttcgtgatcatccgttcctgaaatacggtattctggatattgatcaagag
ccagcgggccaggggtacgcccatcagaaattcgatgtgattgtggcagcgaatgtgatt
cacgcgacccgtgacatccgtgccactgcgaaacgtttgctgagcttgctcgcgccaggc
gggctgctggtgctcgtggaagggaccggccacccgatctggtttgacattacgacgggc
ctgatcgaaggctggcagaaatatgaggatgatctgcgcacggatcatccgctgttgcca
gcacgtacctggtgtgatgtgcttcgccgcgttggcttcgcagatgccgtgagccttccg
ggcgatgggtctccagccgggatcctggggcagcacgtaatcttatcgcgcgcgccaggc
atcgcgggcgctgcttgtgactcaagtggcgagtcggctactgagtctcccgcggcccgg
gccgtccgtcaagagtgggcggatggttcggctgatggcgttcaccgcatggcgctggaa
cgcatgtactttcatcgccgtccaggccgccaggtttgggtgcacggtcgcctccgtaca
gggggcggcgccttcacgaaagcactgacgggcgacctgctgcttttcgaagaaacgggc
caggtggtggctgaggtgcagggcctgcgcctgccgcagcttgaggcatctgcttttgct
ccgcgcgacccacgtgaagagtggttatacgcgctggagtggcagcgcaaagatccgatc
cctgaagcgcctgccgcagcctcatccagcacggcgggcgcgtggcttgttcttatggat
cagggcggcacgggcgcggccttagtgagcctgttggaaggcagaggtgaagcctgcgtt
cgcgtggttgcaggcacagcgtatgcatgcttggcgcctggcctgtatcaggttgatccg
gctcagccagatggctttcatactctgctgcgcgacgcttttggggaagaccgtatgtgc
cgcgcggtggtccacatgtggtcactcgatgctaaagccgctggtgagcgtaccacagcg
gaatcgctgcaagctgaccagctgcttggtagcctgtcggcccttagcctggtgcaggcc
ctggtacggcgccgttggcgcaatatgccgcgtctttggctgctgacgcgtgcagtgcac
gccgtgggtgcggaagacgctgcggcctctgtcgctcaggcaccagtctggggtcttggt
cgcacactcgcactggaacatccggaattacggtgcactctcgtagatgttaatccggcg
ccgagtccagaagatgcggcggcgctggcagttgagttgggcgcgagtgatcgtgaggat
cagattgccctgcgctccaacggtcgctacgttgcccggctggttcgttcaagtttctcc
ggcaagccggcgaccgactgcggcattcgggccgatgggtcatacgtcatcaccgatggg
atgggccgcgttggcctcagcgttgcgcagtggatggttatgcagggcgcgcggcatgtt
gttctcgtggaccgtggcggcgccagtgatgcctctcgtgatgcacttcgctcgatggca
gaagctggtgcggaagtacaaatcgtcgaagcggacgtggcccgccgtgtagatgtagcc
cgtttactgtctaaaattgaaccgagtatgccgccgttgcggggcattgtgtatgtggac
ggtacgtttcagggggattccagcatgttggaactcgatgcccatcgcttcaaagagtgg
atgtatccgaaagttttgggtgcttggaacttgcacgccctgacacgtgaccgtagctta
gattttttcgtcctgtatagcagcggtacatctttactgggccttccgggtcaaggtagc
cgcgccgcaggggatgccttcttagatgcgattgcacatcatcgctgtcgcctaggtctt
accgcgatgtcaattaattggggcctgcttagtgaagccagcagtccggccacgccaaac
gatggtggtgcgcgtctccagtaccgtgggatggaagggcttaccttggagcaaggtgcg
gaagctctgggtcgtttacttgcgcaaccacgcgcgcaggtgggggttatgcgcctgaat
ctccgccagtggctggagttctacccgaatgcggcacgcctggcattatgggcggaactg
ctgaaagaacgtgatcgcaccgatcgcagtgcaagtaacgctagtaacctgcgggaagcg
cttcaatccgcccgcccggaggatcggcagctggttctcgaaaaacacctgtcagaactg
ctgggccgtggtctccgtctgccaccagaacggattgaacgtcatgtcccttttagcaac
ctgggtatggacagtctcattggtttagagctgcgtaaccggattgaagcggccctgggt
attaccgttcctgccactctgctgtggacgtatccgaccgttgccgcactgtccggtaat
ctcctggacattctttctagtaatgctggcgcgacgcatgctccggcgaccgagcgcgaa
aaaagctttgaaaacgacgccgcagatttagaagccttgcgtgggatgactgatgaacag
aaagatgcgctgcttgcggagaaactcgcacaactggcccagatcgtgggcgaagggaat
tcgagctccgtcgacaagcttgcggccgcactcgagcaccaccaccaccaccactga
5' End
3' End
NotesNo accession number for the natural gene was mentioned in the paper, so the record AF217189 was used.
Expression VectorNApET22b, P(BAD)/Myc-HisA (Invitorgen)
Assay MethodsNASDS-PAGE
ResultsNot determinedDetectable expression with P(BAD) promoter.
Protein Functiontype I polyketide synthase
Recoding PurposeTo improve expression
Synthesized ByAuthors
Recoding MethodE. coli preferred codons were used. Gene was designed by Ralph Reid with DNAWorks. Mutations were
introduced to help cloning: I906L; E907Q; L1504S; K1505S; I2398L; E2399Q; F3746S; P3747S.
Publication Author(s)Mutka SC, Carney JR, Liu Y, Kennedy J.
Corresponding AuthorJonathan Kennedy
Corresponding AddressKosan Biosciences, Inc., 3832 Bay Center Place, Hayward, California 94545.
Publication Year2006
Publication TitleHeterologous Production of Epothilone C and D in Escherichia coli.
AbstractThe epothilones are a family of polyketide natural products that show a high potential as anticancer drugs. They are synthesized by the action of a hybrid nonribosomal peptide synthetase/polyketide synthase in the myxobacterium Sorangium cellulosum. In this work, the genes encoding the entire cluster,epoA, epoB, epoC, epoD, epoE, and epoF, were redesigned and synthesized to allow for expression in Escherichia coli. The expression of the largest of the proteins, EpoD, also required the protein be separated into two polypeptides with compatible module linkers. Using a combination of lowered temperature, chaperone coexpression, and alternative promoters, we succeeded in producing a soluble protein from all genes in the epothilone cluster. The entire synthetic epothilone cluster was then expressed in a strain of E. coli modified to enable polyketide biosynthesis, resulting in the production of epothilones C and D. Furthermore, feeding a thioester of the normal substrate for EpoD to cells expressing the epoD, epoE, and epoF genes also led to the production of epothilones C and D. The design of the synthetic epothilone genes together with E. coli expression provides the ideal platform for both the biochemical investigation of the epothilone PKS and the generation of novel biosynthetic epothilone analogues.
JournalBiochemistry. 2006 Jan 31;45(4):1321-30.. 45(4): 1321-30.
SummaryA cluster of epothilone genes were fully codon-optimized towards E. coli codon preference (with DNAWorks). It appeared that the synthetic epothilone genes were successfully expressed to a detectable level.
Comments
Discussion http://www.evolvingcode.net/forum/viewtopic.php?t=505
PubMed ID16430229
Submitter NameWu, Gang
Submitter AddressDepartment of Biological Sciences, University of Maryland Baltimore County, 1000 Hilltop Circle, Baltimore, MD 21250 USA
Entry ConfirmationNo
 
 

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