Synthetic Gene DataBase
 

Synthetic Gene 201


 
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Field NameNatural GeneSynthetic Gene
SGDB Gene ID187201
GenBank AccessionAF217189DQ289496
GenBank GI745355482880274
Gene NameepoFepoF(SYN)
Gene Length (bp)73207380
SpeciesPolyangium cellulosum (Sorangium cellulosum)Escherichia coli
StrainsSMP44K207-3
CDSatggcgaccacgaatgccgggaagcttgagcatgcccttctgctcatggacaagcttgcg
aaaaagaacgcgtctttggagcaagagcggaccgagccgatcgccatcataggtattggc
tgccgcttccccggcggagcggacactccggaggcattctgggagctgctcgactcgggc
cgagacgcggtccagccgctcgaccggcgctgggcgctggtcggcgtccatcccagcgag
gaggtgccgcgctgggccggactgctcaccgaggcggtggacggcttcgacgccgcgttc
tttggcacctcgcctcgggaggcgcggtcgctcgatcctcagcaacgcctgctgctggag
gtcacctgggaagggctcgaggacgccggcatcgcaccccagtccctcgacggcagccgc
accggggtattcctgggcgcatgcagcagcgactactcgcataccgttgcgcaacagcgg
cgcgaggagcaggacgcgtacgacatcaccggcaatacgctcagcgtcgccgccggacgg
ttgtcttatacgctagggctgcagggaccctgcctgaccgtcgacacggcctgctcgtcg
tcgctcgtggccatccaccttgcctgccgcagcctgcgcgctcgcgagagcgatctcgcg
ctggcggggggcgtcaacatgctcctttcgtccaagacgatgataatgctggggcgcatc
caggcgctgtcgcccgatggccactgccggacattcgacgcctcggccaacgggttcgtc
cgtggggagggctgcggtatggtcgtgctcaaacggctctccgacgcccagcgacatggc
gatcggatctgggctctgatccggggttcggccatgaatcaggatggccggtcgacaggg
ttgatggcacccaatgtgctcgctcaggaggcgctcttacgccaggcgctgcagagcgct
cgcgtcgacgccggggccatcgattatgtcgagacccacggaacggggacctcgctcggc
gacccgatcgaggtcgatgcgctgcgtgccgtgatggggccggcgcgggccgatgggagc
cgctgcgtgctgggcgcagtgaagaccaacctcggccacctggagggcgctgcaggcgtg
gcgggtttgatcaaggcggcgctggctctgcaccacgaatcgatcccgcgaaacctccat
tttcacacgctcaatccgcggatccggatcgaggggaccgcgctcgcgctggcgacggag
ccggtgccgtggccgcgggcgggccgaccgcgcttcgcgggggtgagcgcgttcggcctc
agcggcaccaacgtccatgtcgtgctggaggaggcgccggccacggtgctcgcaccggcg
acgccggggcgctcagcagagcttttggtgctgtcggcgaagagcaccgccgcgctggac
gcacaggcggcgcggctctcagcgcacatcgccgcgtacccggagcagggcctcggagac
gtcgcgttcagcctggtagcgacgcggagcccgatggagcaccggctcgcggtggcggcg
acctcgcgcgaggcgctgcgaagcgcgctggaagctgcggcgcaggggcagaccccggca
ggcgcggcgcgcggcagggccgcttcctcgcccggcaagctcgccttcctgttcgccggg
cagggcgcgcaggtgccgggcatgggccgtgggttgtgggaggcgtggccggcgttccgc
gagaccttcgaccggtgcgtcacgctcttcgaccgggagctccatcagccgctctgcgag
gtgatgtgggccgagccgggcagcagcaggtcgtcgttgctggaccagacggcattcacc
cagccggcgctctttgcgctggagtacgcgctggccgcgctcttccggtcgtggggcgtg
gagccggagctcatcgctggccatagcctcggcgagctggtggccgcctgcgtggcgggt
gtgttctccctcgaggacgccgtgcgcttggtggtcgcgcgcggccggttgatgcaggcg
ctgccggccggcggtgcgatggtatcgatcgccgcgccggaggccgacgtggctgccgcg
gtggcgccgcacgcagcgtcggtgtcgatcgcggcagtcaatgggccggagcaggtggtg
atcgcgggcgccgagaaattcgtgcagcagatcgcggcggcgttcgcggcgcggggggcg
cgaaccaaaccgctgcatgtttcgcacgcgttccactcgccgctcatggatccgatgctg
gaggcgttccggcgggtgaccgagtcggtgacgtatcggcggccttcgatggcgctggtg
agcaacctgagcgggaagccctgcacggatgaggtgtgcgcgccgggttactgggtgcgt
cacgcgcgagaggcggtgcgcttcgcggacggcgtgaaggcgctgcacgcggccggtgcg
ggcatcttcgtcgaggtgggcccgaagccggcgctgctcggccttttgccggcctgcctg
ccggatgccaggccggtgctgctcccagcgtcgcgcgccgggcgtgacgaggctgcgagc
gcgctggaggcgctgggtgggttctgggtcgtcggtggatcggtcacctggtcgggtgtc
ttcccttcgggcggacggcgggtaccgctgccaacctatccctggcagcgcgagcgttac
tggatcgaagcgccggtcgatggtgaggcggacggcatcggccgtgctcaggcgggggac
cacccccttctgggtgaagccttttccgtgtcgacccatgccggtctgcgcctgtgggag
acgacgctggaccgaaagcggctgccgtggctcggcgagcaccgggcgcagggggaggtc
gtgtttcctggcgccgggtacctggagatggcgctgtcgtcgggggccgagatcttgggc
gatggaccgatccaggtcacggatgtggtgctcatcgagacgctgaccttcgcgggcgat
acggcggtaccggtccaggtggtgacgaccgaggagcgaccgggacggctgcggttccag
gtagcgagtcgggagccgggggcacgtcgcgcgtccttccggatccacgcccgcggcgtg
ctgcgccgggtcgggcgcgccgagaccccggcgaggttgaacctcgccgccctgcgcgcc
cggcttcatgccgccgtgcccgctgcggctatctatggggcgctcgccgagatggggctt
caatacggcccggcgttgcgggggctcgccgagctgtggcggggtgagggcgaggcgctg
ggcagagtgagactgcctgagtccgccggctccgcgacagcctaccagctgcatccggtg
ctgctggacgcgtgcgtccaaatgattgttggcgcgttcgccgatcgcgatgaggcgacg
ccgtgggcgccggtggaggtgggctcggtgcggctgttccagcggtctcctggggagcta
tggtgccatgcgcgcgtcgtgagcgatggtcaacaggcccccagccggtggagcgccgac
tttgagttgatggacggtacgggcgcggtggtcgccgagatctcccggctggtggtggag
cggcttgcgagcggtgtacgccggcgcgacgcagacgactggttcctggagctggattgg
gagcccgcggcgctcgaggggcccaagatcacagccggccggtggctgctgctcggcgag
ggtggtgggctcgggcgctcgttgtgctcagcgctgaaggccgccggccatgtcgtcgtc
cacgccgcgggggacgacacgagcgctgcaggaatgcgcgcgctcctggccaacgcgttc
gacggccaggccccgacggccgtggtgcacctcagcagcctcgacgggggcggccagctc
gacccggggctcggggcgcagggcgcgctcgacgcgccccggagcccagatgtcgatgcc
gatgccctcgagtcggcgctgatgcgtggttgcgacagcgtgctctccctggtgcaagcg
ctggtcggcatggacctccgaaatgcgccgcggctgtggcttttgacccgcggggctcag
gcggccgccgccggcgatgtctccgtggtgcaagcgccgctgttggggctgggccgcacc
atcgccttggagcacgccgagctgcgctgtatcagcgtcgacctcgatccagcccagcct
gaaggggaagccgatgctttgctggccgagctacttgcagatgatgccgaggaggaggtc
gcgctgcgcggtggcgagcggtttgttgcgcggctcgtccaccggctgcccgaggctcaa
cgccgggagaagatcgcgcccgccggtgacaggccgttccggctagagatcgatgaaccc
ggcgtgctggaccaactggtgctccgggccacggggcggcgcgctcctggtccgggcgag
gtcgagatcgccgtcgaagcggcggggctcgactccatcgacatccagctggcggtgggc
gttgctcccaatgacctgcctggaggagaaatcgagccgtcggtgctcggaagcgagtgc
gccgggcgcatcgtcgctgtgggcgagggcgtgaacggccttgtggtgggccagccggtg
atcgcccttgcggcgggagtatttgctacccatgtcaccacgtcggccacgctggtgttg
cctcggcctctggggctctcggcgaccgaggcggccgcgatgcccctcgcgtatttgacg
gcctggtacgccctcgacaaggtcgcccacctgcaggcgggggagcgggtgctgatccgt
gcggaggccggtggtatcggtctttgcgcggtgcgatgggcgcagcgcgtgggcgccgag
gtgtatgcgaccgccgacacgcccgagaaacgtgcctacctggagtcgctgggcgtgcgg
tacgtgagcgattcccgctcgggccggttcgccgcagacgtgcatgcatggacggacggc
gagggtgtggacgtcgtgctcgactcgctttcgggcgagcacatcgacaagagcctcatg
gtcctgcgcgcctgtggccgccttgtgaagctgggcaggcgcgacgactgcgccgacacg
cagcctgggctgccgccgctcctacggaatttttccttctcgcaggtggacttgcgggga
atgatgctcgatcaaccggcgaggatccgtgcgctcctcgacgagctgttcgggttggtc
gcagccggtgccatcagcccactggggtcggggttgcgcgttggcggatccctcacgcca
ccgccggtcgagaccttcccgatctctcgcgcagccgaggcattccggaggatggcgcaa
ggacagcatctcgggaagctcgtgctcacgctggacgacccggaggtgcggatccgcgct
ccggccgaatccagcgtcgccgtccgcgcggacggcacctaccttgtgaccggcggtctg
ggtgggctcggtctgcgcgtggccggatggctggccgagcggggcgcggggcaactggtg
ctggtgggccgctccggtgcggcgagcgcagagcagcgagccgccgtggcggcgctagag
gcccacggcgcgcgcgtcacggtggcgaaagcggatgtcgccgatcggtcacagatcgag
cgggtcctccgcgaggttaccgcgtcggggatgccgctgcggggtgtcgtgcatgcggca
ggtcttgtggatgacgggctgctgatgcagcagactccggcgcggctccgcacggtgatg
ggacctaaggtccagggagccttgcacttgcacacgctgacacgcgaagcgcctctttcc
ttcttcgtgctgtacgcttctgcagctgggctgttcggctcgccaggccagggcaactat
gccgcagccaacgcgttcctcgacgccctttcgcatcaccgcagggcgcacggcctgccg
gcgctgagcatcgactggggcatgttcacggaggtggggatggccgttgcgcaagaaaac
cgtggcgcgcggctgatctctcgcgggatgcggggcatcacccccgatgagggtctgtca
gctctggcgcgcttgctcgagggtgatcgcgtgcagacgggggtgataccgatcactccg
cggcagtgggtggagttctacccggcaacagcggcctcacggaggttgtcgcggctggtg
accacgcagcgcgcggttgctgatcggaccgccggggatcgggacctgctcgaacagctt
gcctcggctgagccgagcgcgcgggcggggctgctgcaggacgtcgtgcgcgtgcaggtc
tcgcatgtgctgcgtctccctgaagacaagatcgaggtggatgccccgctctcgagcatg
ggcatggactcgctgatgagcctggagctgcgcaaccgcatcgaggctgcgctgggcgtc
gccgcgcctgcagccttggggtggacgtacccaacggtagcagcgataacgcgctggctg
ctcgacgacgccctcgccgtccggcttggcggcgggtcggacacggacgaatcgacggca
agcgccggatcgttcgtccacgtcctccgctttcgtcctgtcgtcaagccgcgggctcgt
ctcttctgttttcacggttctggcggctcgcccgagggcttccgttcctggtcggagaag
tctgagtggagcgatctggaaatcgtggccatgtggcacgatcgcagcctcgcctccgag
gacgcgcctggtaagaagtacgtccaagaggcggcctcgctgattcagcactatgcagac
gcaccgtttgcgttagtagggttcagcctgggtgtccggttcgtcatggggacagccgtg
gagctcgctagtcgttccggcgcaccggctccgctggccgtttttgcgttgggcggcagc
ttgatctcttcttcagagatcaccccggagatggagaccgatataatagccaagctcttc
ttccgaaatgccgcgggtttcgtgcgatccacccaacaagttcaggccgatgctcgcgca
gacaaggtcatcacagacaccatggtggctccggcccccggggactcgaaggagccgccc
tcgaagatcgcggtccctatcgtcgccatcgccggctcggacgatgtgatcgtgcctcca
agcgacgttcaggatctacaatctcgcaccacggagcgcttctatatgcatctccttccc
ggagatcacgagtttctcgtcgatcgagggcgcgagatcatgcacatcgtcgactcgcat
ctcaatccgctgctcgccgcgaggacgacgtcgtcaggccccgcgttcgaggcaaaatga
atggcgacgacgaacgcgggtaaactggaacatgctcttctgttaatggataagctggcg
aagaagaacgcaagtttagagcaggaacgcactgaaccaattgcgattattgggatcggc
tgccgttttccgggtggtgcggacaccccggaagcgttttgggaactgttggatagtggc
cgcgatgctgtgcagccgctggatcgccgttgggcgctggtgggcgtccatccttcagaa
gaagtcccgcgctgggcggggttgctgaccgaggccgtggatgggtttgacgcggcgttc
tttggtacaagtccgcgcgaagcgcgtagcctcgatccgcaacagcgtctgctcctggag
gtaacctgggaaggtctggaagatgccggcatcgcaccgcaatcgctggatggtagccgt
acaggcgtctttcttggggcttgtagctccgactatagccatactgttgcgcagcagcgc
cgcgaagaacaggacgcctatgacattacgggcaacactctttccgtcgctgccgggcgt
ctcagctataccctcggtctacagggcccgtgcctcaccgtagacactgcgtgtagctca
tcgttggtggcaattcacctggcgtgtcgcagcctccgcgcacgcgagtctgatctggcc
ctggctggcggtgttaatatgctgctgtcaagcaaaaccatgatcatgctcggtcgcatt
caagcactgagcccggatggacattgccgtacctttgatgcgtccgctaatggcttcgta
cgcggcgaaggctgcggtatggtggtattaaaacgtctgagcgatgcccagcggcacggc
gatcgcatttgggcattgatccgcggttcagccatgaaccaggacggccgttccaccggg
ttgatggcgccaaacgtcctcgcccaggaagcgctgctgcgtcaggcgctacagagcgca
cgtgtggatgctggcgcgatcgattacgtggagacacatggcacaggcacctcgctgggc
gatccaatagaagttgacgctctgcgtgcagtcatgggtccggctcgtgcggatgggagc
cgttgtgtgttgggtgcagtgaaaacaaacttaggccacctggagggcgccgctggggtg
gcgggtctgatcaaagccgcactggcgcttcaccacgaaagcattcctcgtaatctgcat
ttccacacactcaatccgcgtattcgtattgagggaaccgcgctggccctggcaaccgaa
ccagttccgtggcctcgcgcgggtcgtccacgctttgcgggtgtgtctgctttcggcctg
agtggtaccaacgtgcatgttgtgttggaagaagcacctgccaccgtgttagccccggca
acgccgggccgttctgctgaactgcttgttttaagcgctaaatccacagccgctctggac
gcacaggcggcgcggttatcggcccacatcgcggcatatccggagcaaggtctgggtgat
gtggccttttccttagttgcgacccgcagtccgatggaacatcgtctcgccgttgccgcc
acgtctcgcgaagcgctgcgttctgcgttagaggcggcggcacagggccaaaccccggca
ggcgcggctcgtggtcgtgcggcctcgtcaccgggtaaattggcatttctgttcgctggc
cagggcgcccaagtaccaggtatgggccgtggtctgtgggaagcctggcctgcgtttcgt
gaaaccttcgaccgctgcgttactttgttcgaccgtgagctgcaccaacctctgtgtgaa
gttatgtgggcggaaccgggtagtagccgttcgtcgcttttagaccaaacggcgttcacc
caaccagcgctgttcgcgcttgaatacgcgctggctgcgctgtttagatcttggggcgtg
gaaccggaactgatcgcgggccattctttgggcgagctggtggccgcgtgcgttgcgggc
gtgttttcgctggaagacgctgttcgcttggtggtggcacgcgggcgcctgatgcaggcg
ctgccagctggcggtgccatggttagcattgccgctccggaagccgatgtcgccgcagct
gttgcaccgcacgcggctagtgtctcaatcgccgccgtcaatggccctgagcaggttgtc
attgctggcgcggagaaatttgtgcaacaaattgccgctgcctttgctgcgcgcggtgct
cgcaccaaacctttgcatgtttcccacgcgttccactccccgctgatggatccaatgctg
gaagcatttcgccgcgtcactgaatctgtgacctatcgccgcccgtcgatggcgttagta
agcaatctgtcgggtaaaccgtgtaccgatgaggtgtgtgcgcctggttattgggtacgc
catgctcgggaagcggtgcgcttcgcagatggcgttaaagcgctgcacgcagcaggcgcg
ggtatttttgttgaagttggtccgaaacctgccctgctgggtctgctgcctgcatgtctg
ccggatgcccgtccagtgttactgccagcaagccgcgcaggtcgtgacgaggccgcgtca
gcattagaagcactgggtgggttttgggtggttggtggcagcgtaacgtggagtggtgtg
ttcccgtcaggtggtcgccgtgttcctctcccaacgtatccgtggcaacgggaacggtat
tggctgcaggcacctgtagacggtgaagcggatggtatcggtcgcgcacaagctggcgat
catccattgctgggtgaagccttcagtgtgtcaacccacgcaggtctgcgcctgtgggag
actaccctcgatcgtaaacgtctgccgtggctgggtgagcatcgggcgcagggtgaagta
gtgtttccgggggcaggctacctggaaatggccctttcctcaggcgccgagatattaggg
gatggtccgatccaggtaacggatgtggtgctgattgagaccctgacttttgctggcgat
acggcagttcctgtgcaggttgtgacaactgaagaacgtccgggtcgtctgcggttccag
gtcgcctcccgcgaaccaggggcccgtcgtgcaagttttcgcattcatgcccgtggtgtt
ctgcgtcgcgtcggtcgtgcggaaacgcccgctcgtcttaatctcgccgcactgagagcc
cgcctgcatgcagcagtcccagccgctgctatctatggcgcattggcagaaatggggtta
cagtacgggcctgcactgcgtggtctggcagaactgtggcgtggcgagggtgaagctctg
ggtcgcgttcgtctgccagaatccgcgggttcggcgacagcctatcagctgcacccggtg
ctccttgatgcatgcgtacagatgattgtgggcgcgttcgcggaccgtgatgaagctacg
ccatgggccccggtggaggtcgggagcgtgcgtctcttccaacgctctcctggcgaattg
tggtgccatgcccgtgttgtgtcagacggccaacaggcaccgagtcgctggagcgccgac
tttgagctgatggacggcacaggggctgtagttgcagagattagccgtctggtggttgaa
cgcttagcgtccggcgtccgccgccgtgacgcggacgattggtttctggagctcgattgg
gaaccggcagcattagagggtccgaaaatcacggccggtcgctggctgctgctgggggag
ggtgggggcttgggccgttctttatgtagtgcgctgaaagcggctggtcatgttgtggta
cacgccgcaggggatgatacgtctgcggcaggcatgcgtgcgttgctggcgaacgcgttc
gatggtcaggcgccgacggctgtcgtccacctcagctctctggacggcggcggtcaactg
gatcctggcttgggcgctcaaggcgcattggacgctccgagatctccagacgtggacgca
gacgcccttgagtccgcattaatgcgcggttgcgattccgtgctgagcctggtgcaggcg
ctcgtcggtatggatctgcggaacgcaccacgtctgtggctgcttacccgtggcgcacag
gcagctgccgcaggcgatgtctcggtggtgcaggctccgctgctggggctgggccgcacg
atcgcgctggaacatgcagaacttcgctgtatctcagtagatttggatccggcacagccg
gaaggcgaagcggacgcgctgctggccgaactgctggctgacgacgcggaggaagaagtg
gcattgcgtggtggtgaacgctttgtggcacgtctggttcaccgcttgccggaagcgcaa
cgtcgggaaaaaattgcgccagcgggcgaccgcccgtttcgcttggaaatcgatgaaccg
ggtgttttagatcagttagttcttcgtgcaacgggtcgccgtgcgccgggcccgggcgaa
gtcgagatcgccgtagaggctgcgggcctggattctattgatattcagcttgccgtcggg
gtagcaccgaacgacttgcctggcggggagatcgagccgtcggtcctgggtagtgaatgc
gccggccgcatcgtagcagtaggtgaaggcgtgaatgggttggtagtgggtcagccggtt
attgccttagcggcgggtgtttttgcgacgcatgttacgacttctgcgaccctggtgctg
ccgcgtccgctcgggttgagcgcgaccgaagcggcggcgatgccattggcgtatcttacc
gcttggtatgcgcttgataaagttgctcaccttcaggcaggcgaacgtgttctgattcgg
gcggaggccgggggcattggtctgtgcgccgtccggtgggcgcagcgcgttggtgctgag
gtctatgcgaccgccgacacgccagaaaaacgtgcctaccttgagtcgctgggtgtgcgc
tacgtgagcgattctaggtctggtcgcttcgcagcggatgtccatgcgtggaccgatggg
gagggcgttgatgtggttctggactctctgtccggcgaacatatcgataaaagtctgatg
gttttacgcgcatgtgggcgcctcgttaaactgggtcgccgtgacgattgcgctgacacc
caaccagggctgccaccgttgttgcgcaacttttcattttctcaggtggatctgcgtggc
atgatgctggaccagcccgcgcggattcgtgctcttctggatgaattgtttggcctggtg
gcggccggtgcgatttcccctttagggagcggtctgcgggttggtggcagcctgaccccg
ccacctgtcgaaaccttcccaattagtcgtgccgctgaagccttccgtcgcatggcgcag
ggtcagcatctcggtaaactggtcctgaccctggatgatccagaggttcgtattcgtgcg
ccagccgaaagcagcgtggcagttcgtgcagatggcacctatttagttaccggtggttta
ggtggcttgggcttacgtgttgctggctggctggcagaacgcggtgctgggcagttagtg
ttagtgggccgtagcggcgctgcctccgcagaacagagagccgccgtggccgccctggag
gcccatggcgcccgcgtcaccgtagctaaagctgatgtagcggatcgttcacaaattgaa
cgcgtactgcgcgaagtcacggcttccggcatgccgctgcggggcgttgtccacgccgct
ggtttagtagacgacggcctgttgatgcaacagaccccggcccgccttcgtacggtaatg
ggccctaaagtgcaaggtgcccttcatctgcacactctgactcgggaagcacctttatct
ttctttgttctgtatgcaagtgcagcaggtttattcggcagcccgggtcagggtaattac
gctgctgcaaacgcttttctggatgcgctgagtcatcaccggcgtgcgcatgggttgcca
gccttaagcattgactggggcatgtttaccgaagtggggatggcggtcgcacaagagaac
cgtggcgcacgccttattagtcggggcatgcgcggtattacgccggacgaagggctgtca
gcgttggcccgccttctcgaaggtgatcgtgttcaaacgggtgtgatcccgattacaccg
cgtcagtgggtggagttctatccggccacagcggccagtcgtcgtctcagccgcctggtc
acaactcagcgtgcggtcgctgatcgcaccgccggggatcgcgatctcctcgaacagttg
gcctcggcggaaccatccgctcgggctggcctgttgcaagatgtcgtacgcgtgcaggtg
tcgcatgtgctccgcctgccggaggataaaatcgaggtggacgcaccgttatccagtatg
ggtatggatagtttgatgtcgctggaattacgcaatcgtatcgaagccgcgctgggcgta
gcggctccggcagctctgggttggacttacccgacggtggcagctattacccgttggtta
ctggatgatgctctttctagtcgcttaggcggcgggagcgatacggatgaatccactgca
tcggcgggtagctttgttcacgtcctgcgttttcgcccggtagtaaaaccgcgtgcacgc
ctgttttgttttcacggttcggggggttctccagaaggcttccgtagctggtctgaaaaa
tcagagtggagtgacctcgaaattgtcgcgatgtggcatgatcgttccttggcatctgag
gatgccccgggcaaaaaatatgttcaggaagctgccagtctcatccaacattatgcggat
gccccatttgctcttgtgggtttctctttgggtgttcgctttgtaatgggcacagcggtg
gagctggcttctcggagtggggcgccagcaccattggcggtgttcgcactgggtggctcc
ctgatttccagcagcgaaatcactccggagatggagaccgatattatcgcgaaactgttt
tttcgtaacgcggccggtttcgtgcgctcaacacagcaagtccaggctgacgcccgcgcg
gataaagtgattactgataccatggtcgcccctgcgccgggtgatagcaaagaaccgccg
tcaaaaatcgcggtgccgatcgttgcaattgccggttcggatgacgtgatcgtccctcca
tcggacgttcaggacttacagagccgtaccaccgaacggttttacatgcatctgctgccg
ggcgaccatgagttcctggttgaccgcgggcgtgaaattatgcatattgtagattcacac
cttaatccgctgttagctgcccgcaccacgtccagtggcccggccttcgaagcaaaaggg
aattgtagctccgtcgacaagcttgcggccgcactcgaacaccaccaccaccaccactga
5' End
3' End
NotesNo accession number for the natural gene was mentioned in the paper, so the record AF217189 was used.
Expression VectorNAP(BAD)/Myc-HisA (Invitorgen)
Assay MethodsNASDS-PAGE (Coomassie blue staining)
ResultsNot determinedDetectable expression
Protein Functiontype I polyketide synthase
Recoding PurposeTo improve expression
Synthesized ByAuthors
Recoding MethodE. coli preferred codons were used for the whole gene. Designed by ralph Reid with DNAWorks. To
facilitate cloning, amino acid substitutions were introduced at I902L; E903Q; A2166S; V2167S
Publication Author(s)Mutka SC, Carney JR, Liu Y, Kennedy J.
Corresponding AuthorJonathan Kennedy
Corresponding AddressKosan Biosciences, Inc., 3832 Bay Center Place, Hayward, California 94545.
Publication Year2006
Publication TitleHeterologous Production of Epothilone C and D in Escherichia coli.
AbstractThe epothilones are a family of polyketide natural products that show a high potential as anticancer drugs. They are synthesized by the action of a hybrid nonribosomal peptide synthetase/polyketide synthase in the myxobacterium Sorangium cellulosum. In this work, the genes encoding the entire cluster,epoA, epoB, epoC, epoD, epoE, and epoF, were redesigned and synthesized to allow for expression in Escherichia coli. The expression of the largest of the proteins, EpoD, also required the protein be separated into two polypeptides with compatible module linkers. Using a combination of lowered temperature, chaperone coexpression, and alternative promoters, we succeeded in producing a soluble protein from all genes in the epothilone cluster. The entire synthetic epothilone cluster was then expressed in a strain of E. coli modified to enable polyketide biosynthesis, resulting in the production of epothilones C and D. Furthermore, feeding a thioester of the normal substrate for EpoD to cells expressing the epoD, epoE, and epoF genes also led to the production of epothilones C and D. The design of the synthetic epothilone genes together with E. coli expression provides the ideal platform for both the biochemical investigation of the epothilone PKS and the generation of novel biosynthetic epothilone analogues.
JournalBiochemistry. 2006 Jan 31;45(4):1321-30.. 45(4): 1321-30.
SummaryA cluster of epothilone genes were fully codon-optimized towards E. coli codon preference (with DNAWorks). It appeared that the synthetic epothilone genes were successfully expressed to a detectable level.
Comments
Discussion http://www.evolvingcode.net/forum/viewtopic.php?t=505
PubMed ID16430229
Submitter NameWu, Gang
Submitter AddressDepartment of Biological Sciences, University of Maryland Baltimore County, 1000 Hilltop Circle, Baltimore, MD 21250 USA
Entry ConfirmationNo
 
 

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