BIOT

DIVISION OF BIOCHEMICAL TECHNOLOGY

Final Program, 225th ACS National Meeting, New Orleans, LA, March 23-27, 2003

OTHER SYMPOSIA OF INTEREST: Gene-Based Medicine: Delivery and Diagnostics (see Division of Polymeric Materials: Science and Engineering, Tue) New Concepts in Biomaterials: Synthesis, Application, Concerns (see Division of Polymeric Materials: Science and Engineering, Sun, Mon) Polymeric Surface Modification: Biomedical Applications (see Division of Polymer Chemistry, Sun, Mon) Polymeric Surface Modification: Biomedical Applications (see Division of Polymer Chemistry, Sun) MALDI and ESI Mass Spectrometry Techniques for Polymers (see Division of Polymeric Materials: Science and Engineering, Sun, Mon) Macromolecules to Bioparticles: Analyses with Field-Flow Fractionation (see Division of Analytical Chemistry, Mon) Adsorption of Macromolecules at Liquid-Solid Interfaces (see Division of Colloid and Surface Chemistry, Sun, Mon, Tue) Biological Applications of Implicit Solvent Models (see Division of Computers in Chemistry, Mon, Wed, Thu) Microscale Biosensors for Environmental Monitoring (see Division of Environmental Chemistry, Mon) Biological Applications of Nanomaterials and Nanotechnology (sponsored by Advanced Materials & Nanotechnology Subdivision) (see Division of Industrial and Engineering Chemistry, Wed, Thu) Informatics Challenges in Pharmacogenomics (see Biotechnology Secretariat, Wed) Processes for Utilization of Agricultural By-Products (see Division of Agricultural & Food Chemistry, Wed, Thu) SOCIAL EVENTS: Annual BIOT Reception: Sun BIOT Business Meeting: Wed BIOT Future Programming Meeting: Tue Poster Session: Wed

SUNDAY MORNING

 Section A

Collection, Analysis, and Use of Process Data

8:00 — Introductory Remarks.

8:05 —1. Managing and analyzing bioprocess manufacturing data for process troubleshooting, statistical quality control, and regulatory submissions. S. Illich, N. Gerrior, P. Werth

8:30 —2. Critical assessment on the analysis of batch fermentation databases. C. Undey, A. Cinar

8:55 —3. On-line monitoring of bioprocesses using multiwavelength fluorescence. A. Eliasson, M. Haack, L. Olsson

9:20 —4. Identifying key variables for cell culture process data analysis utilizing principal component analysis and basis transformation. S. Kaneshiro, T. Monica, R. Kiss

9:45 — Intermission.

10:05 —5. Retrospective time-dependent optimization of recombinant E. coli fermentations using historical data and hybrid neural network models. M. C. Coleman, D. E. Block

10:30 —6. Use of statistical experimental design methods in developing a high-concentration liquid formulation for a monoclonal antibody. E. Kaisheva

10:55 —7. Integrated process optimization for bioparticle production. P. E. Cruz, A. Bengala, L. Maranga, M. J. T. Carrondo

 Section B

Advances in Formulation

8:00 — Introductory Remarks.

8:05 —8. Correlation of physical and chemical stability of rhDNase with melting temperature. M. E. M. Cromwell, T. Patapoff, S. J. Shire

8:25 —9. Development of a stable parenteral formulation for Pemphigus vulgaris tolerizing peptide (PI-0824). J. Ghiorse, L. Waldmann, A. Trischitta, T. Sawyer, E. Clark

8:45 —10. Characterization of albutropin fusion protein: A novel, long-acting form of human growth hormone. A. M. Wilcox

9:05 —11. Stabilization of an oral rotavirus vaccine. C. Burke

9:25 —12. Designing an in vitro cell cell culture model to predict drug pharmacokinetics and stability. S. F. Khattak, S. C. Roberts

9:45 — Intermission.

10:10 —13. Characterization of a novel adjuvant/delivery system for plasmid DNA vaccines. R. K. Evans, H. Mach, D. Zhu, R. D. Troutman, D. R. Casimiro, S. Chin, S. Wu, C. Ahn, D. K. Nawrocki, L. A. Isopi, D. M. Williams, D. B. Volkin, J. W. Shiver

10:30 —14. Sterilizing filtration of plasmid DNA: Effects of plasmid molecular weight and conformation. M. P. Watson, D. Boyd, J. C. Murphy, S. Sagar, R. K. Evans, A. L. Lee, M. Winters

10:50 —15. Effects of formulation components on the corrosion of 316L stainless steel. P. Lam, R. Keck, S. Lo, R. Wong, C. Hsu

 Section C

Environmental Biotechnology

8:00 — Introductory Remarks.

8:05 —16. Enhanced solubility of TCE and other contaminants due to the presence of biological material. J. W. Barton, S. A. Jones, C. Vodraska, B. H. Davison

8:25 —17. Use of fluorinated analogs to explore pollutant fate in plant systems. J. M. Tront, F. M. Saunders

8:45 —18. Enzymatic treatment of phenol with crude fungal peroxidase from Coprinus species. K. Ikehata, I. D. Buchanan, D. W. Smith

9:05 —19. Microbial identification using "signature probes". M. Larios-Sanz, K. D. Kourentzi, G. E. Fox, R. C. Willson

9:25 —20. An alternative ecological approach to process control optimization for bioprocesses operated with mixed microbial populations. P. E. Saikaly, D. B. Oerther

9:45 — Intermission.

10:05 —21. Detoxification of organophosphates by immobilized dual functional biocatalyst in a cellulose hollow fiber bioreactor. A. A. Wang, W. Chen, A. Mulchandani

10:25 —22. Two-phase partitioning bioreactors: A new paradigm for xenobiotics destruction in air, water, and soil. A. J. Daugulis, B. G. Amsden

10:45 —23. Influence of CO₂ concentration in air on algae respiration in membrane bioreactor. L. M. Cao, H. L. Chen, C. Y. Gao, L. H. Cheng, J. Y. Feng

Polymeric Surface Modification: Biomedical Applications

SUNDAY AFTERNOON

 Section A

Advances in Expression Systems

1:30 — Introductory Remarks.

1:35 —24. Mimicking the cytoplasmic environment of Escherichia coli with cell-free protein synthesis activates oxidative phosphorylation. M. C. Jewett, J. R. Swartz

1:55 —25. Cell-free protein synthesis utilizing unnatural amino acids. J. Schulte, J. R. Swartz

2:15 —26. Export of correctly folded antibodies from the cytoplasm of Escherichia coli via the twin-arginine translocator. M. P. DeLisa, D. Tullman, G. Georgiou

2:35 —27. NusA fusion protein expression and purification in conventional and high-throughput applications. A. Grabski, M. M. Mehler, K. W. Yaeger, K. Novy Jr., G. D. Davis, R. G. Harrison

2:55 —28. Engineering yeast for high-level expression of G-protein coupled receptors. A. S. Robinson, R. T. Niebauer, J. Butz

3:15 — Intermission.

29. Withdrawn.

3:45 —30. Modification of the N-glycosylation pathway of lower eukaryotes to a mammalian type. R. H. Contreras, W. Vervecken, N. Callewaert, S. Geysens, V. Kaigorodov

4:05 —31. Expression of human antibodies for clinical supply using myeloma cells. L. Daramola, R. Field

 Section B

Advances in Protein Folding, Aggregation, and Modification

1:30 — Introductory Remarks.

1:35 —32. Effect of secondary structure on protein aggregation: A replica exchange on-lattice simulation. D. Bratko, H. W. Blanch

1:55 —33. Critical role of hydration in enzyme activity in organic solutions. L. Yang, J. S. Dordick, S. Garde

2:15 —34. Protein folding and proton binding are linked: Analysis by capillary electrophoresis and electrokinetic models of colloids. J. D. Carbeck

2:35 —35. Aggregation during refolding is dependent on the second virial coefficient. J. G. S. Ho, A. P. J. Middelberg

2:55 —36. Strategies for modulating aggregation kinetics of amyloid-forming peptides. R. M. Murphy, J. R. Kim, T. J. Gibson, L. L. Kiessling

3:15 — Intermission.

3:25 —37. Multiple active forms of single-chain antibody: Characterization and folding pathway analysis. A. S. Robinson, J. R. Sinacola

3:45 —38. Structure, function, and engineering of bacterial disulfide isomerases for enhancing heterologous protein expression. L. Segatori, G. Georgiou

4:05 —39. Exploring the dimer interface as element of kinetic stability in a bacterial starch phosphorylase. B. Nidetzky, R. Griessler, A. Schwarz, F. Tanfani, A. Scire

4:25 —40. Analysis of cross-linking hemoglobins using variable-length reagents. E. Tarasov, K. M. Bobofchak, G. S. Gawlak, K. P. Normoyle, R. M. Danner, K. W. Olsen

 Section C

Bioprocess Monitoring and Control

1:30 — Introductory Remarks.

1:35 —41. Application of the focused beam reflectance measurement method to the characterization of plant cells in suspension culture. P. T. Jeffers, S. Raposo, M. E. Lima-Costa, P. Kieran, B. Glennon

1:55 —42. Growth sensitivity of microbial populations in mixed cultures is determined by a new microrespirometry method. P. G. Stroot, D. B. Oerther

2:15 —43. On-line viable cell monitoring during long-term microcarrier cultures. F. Jimenez-Marrero, L. Bonnarens, J. L. Barker, J. E. Fleury, M. A. Forbes, R. N. Pattison, C. W. Buser, C. Hwang

2:35 —44. On-line biomass monitoring with scanning dielectric spectroscopy. C. M. Cannizzaro, S. Valentinotti, I. W. Marison, U. von Stockar

2:55 —45. A mechanism for glucose pH control. M. A. Alexander, J. G. Hoogerheide, C. M. Campbell

3:15 — Intermission.

3:35 —46. Novel sensors for cell culture and fermentation. G. Rao, X. Ge, Y. Kostov, L. Tolosa

3:55 —47. Adaptive control of a G. xylinus fed-batch fermentation using in situ mid-IR spectroscopy. H. Kornmann, S. Valentinotti, M. H. Rhiel, U. von Stockar

4:15 —48. Real-time fault detection, diagnosis, and quality prediction in batch fermentations. C. Undey, S. Ertunc, E. Tatara, A. Cinar

Polymeric Surface Modification: Biomedical Applications

Tutorial

SUNDAY EVENING

 Section A

David Perlman Memorial Lecture

5:00 —49. Title to be announced. T. To Be Announced

Polymeric Surface Modification: Biomedical Applications

MONDAY MORNING

 Section A

Advances in Polypeptide Production Technology: Accelerating Product and Process Development

8:00 — Introductory Remarks.

8:05 —50. Accelerating product and process development by using cell-free protein synthesis. J. R. Swartz, N. Michelle-Reydellet, K. Knapp, G. Yin, J. Yang

8:30 —51. Enhancement of antibody fragments expression in Escherichia coli: A novel cytoplasmic screening approach. R. Levy, B. Iverson, G. Georgiou

8:55 —52. Engineering fungal systems to produce human glycoproteins. T. Gerngross

9:20 —53. Overexpression of industrial enzymes in Pichia pastoris. J. Li, T. Kaneko, C. Isaac, N. Palackal, J. Wei, J. Sun, B. Steer, A. Vasavada

9:45 — Intermission.

10:00 —54. Simulation of large-scale environments in the laboratory. T. Dodge, P. Lundqvist

10:25 —55. Oxidative stress responses in E. coli fermentations under controlled conditions. C. Lu, W. E. Bentley, H. J. Cha, J. Garcia, G. Rao

10:50 —56. Accelerating development of mammalian cell bioprocesses for recombinant protein production. C. Goudar, R. Heidemann, J. Michaels, K. Konstantinov

 Section B

Therapeutic Antibody Issues: Fundamentals and Production Processes

8:00 — Introductory Remarks.

8:05 —57. Challenges of securing biologics capacity. E. Bramhall, S. S. Lee, J. Tabor

8:30 —58. Development of a robust fed-batch CHO cell culture platform to optimize monoclonal antibody production. P. A. Garza, C. Kopelev, D. Osborne, M. Reid, J. Torres, M. Gaya, N. Vargas, W. Noe, Y. H. D. Chang

8:55 —59. Improved process yields in monoclonal antibody manufacture. A. E. Schmelzer, S. White, E. Tsao, A. Varma

9:20 —60. The role of process simulation in the development, evaluation, and debottlenecking of monoclonal antibody production processes. C. Siletti, D. Petrides

9:45 — Intermission.

10:00 —61. Characterization of a small molecule conjugated antibody. W. Zhang

10:25 —62. Development of high-performance IDEC's in-house medium for antibody production. Y. Huang, T. Huynh, L. Ly, W. Noé, Y. H. D. Chang

10:50 —63. Impacts of glycosylation on antibody effector function. V. Sarantschin

11:15 — Panel Discussion.

 Section C

Evaluation of Column Performance in Preparative Chromatography

8:00 — Introductory Remarks.

8:05 —64. How can you tell if a column is doing its job? E. N. Lightfoot

8:35 —65. Monitoring, diagnosis, and troubleshooting of large-scale chromatography. J. Prior, M. Menon, C. Dugopolski, K. Legg

8:55 —66. Packing quality and integrity of industrial chromatography columns: From raw data smoothing to performance correlations. A. Sonnenfeld, H. Forrester, J. Thommes

9:15 —67. Determination of the parameters governing biomolecule transport in EDTPA-modified zirconia particles. A. Subramanian, S. Sarkar, P. W. Carr

9:35 —68. Chemometric analysis of chromatography production data: Integrating principal component analysis into a highly regulated industry. M. M. Mostajo, T. M. Larson, J. O. Davis

9:55 — Intermission.

10:10 —69. Resin reuse validation and evaluation of performance over resin lifetime. T. S. Taggart, D. J. Dripps, T. Kessler, M. E. Cameron, J. E. Seely, R. J. Todd

10:30 —70. Evaluation of algorithms for quantification of packed-bed integrity in production-scale chromatography. J. O. Davis, T. M. Larson, R. Arnold

10:50 —71. Tracking resin dynamic capacity in preparative chromatography. R. S. Blackmore, J. R. Ryland

 Section D

2002 Industrial Biotechnology Awards Presentation and Lecture

11:40 —72. Title to be announced. T. To Be Announced

Polymeric Surface Modification: Biomedical Applications

MONDAY AFTERNOON

 Section A

Advances in Fermentation Process Development

2:00 — Introductory Remarks.

2:05 —73. Seed train development for production of HIV gag plasmid in E. coli. J. L. Okonkowski, R. Greasham, M. Chartrain

2:30 —74. Integrated systems approach for the optimization of recombinant fermentation processes. K. Bayer, M. Cserjan, G. Striedner, K. Duerrschmid, H. Reischer, F. Clementschitsch

2:55 —75. Process development by CFD modeling for very large-scale fermentations: Development of yeast kinetics for batch processes. C. G. Hébert, C. E. Wyman, L. R. Lynd, A. Bakker

3:20 —76. Monitoring cell populations in bioreactors. J. Kacmar, N. Vijayasankaran, F. Srienc

3:45 — Intermission.

4:10 —77. Production of novel triketide lactone analogs by directed biosynthesis. R. Regentin, J. Kennedy, N. Wu, J. Galazzo, P. Licari, R. Desai

4:35 —78. Enhanced protein production by Bacillus subtilis using a dual exponential feeding strategy. H. Huang, D. Ridgway, T. Gu, M. Moo-Young

5:00 —79. Improvement in oxygen transfer in submerged fermentation using microbubbles. J. Weber, F. Agblevor

5:25 —80. Development and scale-up of Pichia pastoris fermentation and protein recovery processes. D. B. Rudolph, T. Powers, R. Land, S. Pittinger, D. R. Durham

 Section B

Therapeutic Antibody Issues: Fundamentals and Production Processes

2:00 — Introductory Remarks.

2:05 —81. Effects of anti-apoptosis genes on mammalian cells producing antibodies. B. Figueroa Jr., E. Ailor, M. Reff, J. M. Hardwick, M. J. Betenbaugh

2:25 —82. Production of proteins with complex glycosylation in insect cells. C. E. Joosten, M. L. Shuler

2:45 —83. Analysis of metabolic decline in batch/fed-batch hybridoma cultures. R. R. Balcarcel, L. M. Clark

3:05 —84. Production of antibodies and antibody fragments in Aspergillus niger. H. J. Meerman, H. Wang, C. Lin, D. Victoria, M. Ang, J. Pucci, M. Heng, X. Wang, R. Fong, B. Fox, B. Fryksdale, D. Wong, M. Ward

3:25 —85. Engineered antibodies to treat whooping cough. J. Maynard, D. Relman, B. Iverson, T. Merkel, G. Georgiou

3:45 — Intermission.

4:05 —86. Toward production of therapeutic antibodies with mammalian-type glycosylation in fungal systems. S. Wildt

4:25 —87. Evaluation of a continuous disc stack centrifuge for the clarification of mammalian cell cultures. R. Shpritzer

4:45 —88. CE: A rapid analysis tool and an emerging technology. D. L. Beurer, D. J. Roush, K. E. Goklen

5:05 — Discussion.

 Section C

Advances in Chromatography

2:00 — Introductory Remarks.

2:05 —89. Chromatography of ultralarge proteins and nanoparticles using monoliths. A. Jungbauer, A. Zoechling, R. Hahn

2:25 —90. Stimulus-responsive polymers used in chromatographic separation. R. Palmgren, J. Van Alstine, A. Rudstedt, A. Kjellgren, M. Wulff

2:45 —91. Protein and stationary-phase determinants of FGF retention on cation exchangers. A. Lehnoff, P. DePhillips, Y. Yao

3:05 —92. Modeling of breakthrough curves of staphylococcal enterotoxin B in peptide affinity chromatography. G. Wang, R. G. Carbonell

3:25 —93. Strategies for maintaining chromatographic column performance across scales. A. Williams, K. Taylor, K. Dambuleff, R. Kennedy

3:45 — Intermission.

4:15 —94. Purification of plasmid DNA on a novel matrix using ion-pair chromatography. J. Curling, M. Chambers, L. Smiley, A. Whan, D. Baines

4:35 —95. Qualification of large-scale chromatography systems. O. Kaltenbrunner, A. Stokelman

4:55 —96. Novel software tools for evaluating integration within a downstream process: A case study of expanded-bed adsorption vs packed-bed adsorption. M. A. Mustafa, J. Washbrook, S. Farid, A. C. Lim, N. Titchener-Hooker

 Section D

Novel Bioanalyses Using Lab-on-a-Chip Technologies

2:00 — Introductory Remarks.

2:05 —97. Biomediated assembly of functionalized microbeads for capture of microorganisms. T. T. Huang, T. Geng, D. Akin, W. Chang, J. Sturgis, R. Bashir, A. K. Bhunia, J. P. Robinson, M. R. Ladisch

2:25 —98. Biomembranes on a microfluidic chip. P. Cremer

2:55 —99. Development of a biomimetic circulatory system using chips fabricated from PDMS. D. J. Fischer, N. J. Torrence, D. M. Spence

3:15 —100. Microfluidic devices for complex sample analysis: Integrated on-chip sample preparation, cellular, and chemical analysis starting from raw samples. B. H. Weigl

3:45 — Intermission.

4:05 —101. Polymer-based microfluidic devices for PCR amplification of genomic DNA. S. A. Soper, R. L. McCarley, M. C. Murphy

4:35 —102. Microreactors without microfabrication: Using microscale steady-state kinetic analysis to study biological interactions. N. J. Gleason, J. D. Carbeck

4:55 —103. Microfluidic sensors for protein analyses. G. Thomas, M. J. Tarlov

MONDAY EVENING

 Section A

Sci-Mix Poster Session

8:00 - 10:00

223-224, 226, 229-230, 232, 234, 242, 244-245, 253, 255, 258, 260, 262-266, 268-269, 275, 279, 285, 287, 290, 293, 296, 300, 302, 304, 311, 315, 325-328, 331-332, 335. See subsequent listings.

TUESDAY MORNING

 Section A

Bioinformatics, Genomics, and Proteomics

8:00 — Introductory Remarks.

8:20 —104. Metabolic flux analysis based on dynamic genomic information. K. San, S. Cox, G. N. Bennett

8:40 —105. Metabolite and protein profiling of whole cells using soft-ionization mass spectrometry for rapid microbial characterization. S. Vaidyanathan, R. Goodacre

9:00 —106. Dynamic modeling of the Escherichia coli phosphoenolpyruvate-glycose phosphotransferase system. P. Prakash, J. S. Edwards

9:20 —107. Transcriptome analysis of ethanologenic Escherichia coli strains: Tolerance to ethanol. R. Gonzalez, H. Tao, K. T. Shanmugam, L. O. Ingram

9:40 — Intermission.

9:55 —108. A functional proteomics study in neurobiology and the elucidation of signal transduction pathways. W. D. Mo

10:15 —109. Glycoside hydrolases from Thermotoga maritima: Physiological and biotechnological aspects. S. R. Chhabra, K. R. Shockley, S. B. Conners, K. Scott, R. D. Wolfinger, R. M. Kelly

10:35 —110. Protein classification by ligand binding. M. Farnum, S. Izrailev

10:55 —111. A computational framework for the discovery of novel biotransformations. V. Hatzimanikatis, C. Li, J. A. Ionita, L. J. Broadbelt

 Section B

Advances in Bioseparations

8:00 — Introductory Remarks.

8:05 —112. Biofunctional membranes for Listeria monocytogenes detection. W. Chen, M. R. Ladisch, T. Geng, A. K. Bhunia

8:25 —113. Enhanced ultrafiltration: Using electrostatic repulsion to increase yield and throughput. G. Bolton, R. Kuriyel, S. Pearl

8:45 —114. Optimization of an osmotic shock procedure for isolation of a protein product expressed in E. coli. A. S. Rathore, R. E. Bilbrey, D. E. Steinmeyer

9:05 —115. Purification of a polysaccharide conjugate vaccine using microfiltration membrane in backpulsing mode. E. P. Wen, L. D. D'Silva, D. Murray, S. L. Sagar, A. L. Lee

9:25 —116. Membrane adsorber technology for trace impurity removal applications. M. W. Phillips, H. Lutz

9:45 — Intermission.

10:15 —117. Membrane adsorber process optimization through design of experiments. J. Pieracci, C. Schifftner, J. Thommes, L. E. Conley

10:35 —118. Strategies for optimizing the robustness of viral filtration steps in large-scale biopharmaceutical manufacturing processes. R. Caren

10:55 —119. Strategies for improving mammalian cell clarification performance. S. Abraham, N. Bingham, K. Green, J. Kenworthy

 Section C

Advances in Vaccine Development, Gene Delivery, and Gene Therapy

8:00 — Introductory Remarks.

8:05 —120. Development and characterization of an improved adenoviral gene therapy manufacturing process. C. K. Murphy, J. M. Keegan, D. McNeilly

8:30 —121. Charged poly(lactide-co-glycolide) microparticles can effectively deliver proteins and DNA in vaccines. J. D. Chesko, J. Kazzaz, M. Ugozzoli, M. Singh, D. O'Hagan

8:55 —122. Developing an AAV-based vaccine for HIV-1. B. A. Thorne

9:20 —123. High-titer production of retroviral vector with suspension-adapted 293GPG cells. A. Garnier, K. Ghani, P. Trudel, A. Kamen

9:45 — Intermission.

10:05 —124. Protection against anthrax toxin by heteropolymers directed against protective antigen. R. Mabry, C. Maassen, N. Mohamed, K. Brasky, J. Pattersen, G. Spitalny, L. Casey, B. L. Iverson, G. Georgiou

10:30 —125. Quantitative comparison of nonviral gene delivery vectors: Model analysis of intracellular trafficking events. C. M. Varga, R. Langer, D. A. Lauffenburger

10:55 —126. Development of a manufacturing process for a cell culture-derived smallpox vaccine. F. Wu, G. Seanez, R. Kiefer, J. Marshall, S. Kodihalli, J. Gilly, D. Fine, D. Clanton

 Section D

Marvin J. Johnson Award Presentations and Lecture

11:40 —127. De novo design of protein secretion pathways for profit and for fun. G. Georgiou

TUESDAY AFTERNOON

 Section A

Bioinformatics, Genomics, and Proteomics

2:00 — Introductory Remarks.

2:20 —128. Predicting operon and regulon structure in Archaeoglobus fulgidus using transcriptomic data. L. Rohlin, C. Sabatti, J. C. Liao

2:40 —129. Genome-wide analysis of protein export via the Tat pathway in Escherichia coli. D. Tullman, M. P. DeLisa, Y. Kawarasaki, G. Georgiou

3:00 —130. Global effects of oxygen exposure during recombinant protein production in Escherichia coli. W. B. Perry, C. L. Cooney

3:20 —131. Analysis of mRNA and protein expression in Escherichia coli. K. H. Lee, P. S. Lee

3:40 — Intermission.

4:10 —132. Proteomic analysis of metabolic changes in fed-batch CHO cell cultures. D. E. Pascoe, D. Arnott, W. M. Miller, E. T. Papoutsakis, D. C. Andersen

4:30 —133. Metabolic and evolutionary engineering in the era of genomics: Antibiotic resistance as a model for engineering new microbial behaviors. R. T. Gill, S. Copley, M. Lynch

4:50 —134. Functional genomics in tissue engineering: Gene expression profile of engineered skin equivalents subjected to barrier disruption. S. T. Andreadis, P. Koria, D. Brazeau, P. Hayden

5:10 —135. Pattern discovery as a generic tool for the analysis of biological data. K. Jensen, G. N. Stephanopoulos

 Section B

Advances in Bioseparations

2:00 — Introductory Remarks.

2:05 —136. Comparison of strong cation-exchange resins for protein purification. A. Staby, I. H. Jensen, U. Bruus, M. Sand, R. G. Hansen, J. H. Jacobsen

2:25 —137. New chromatofocusing methods in biotechnology. D. Frey, X. Kang, H. Shen

2:45 —138. Practical aspects of large-scale protein crystallization. M. Heng

3:05 —139. Effect of support characteristics on the performance of protein A chromatography media. J. T. McCue, I. Quiñones-Garcia, G. Kemp, D. Low

3:25 —140. Efficiency of expanded-bed adsorption processes. R. A. Hjorth

3:45 — Intermission.

4:15 —141. Multiobjective optimization strategies for preparative chromatography. D. Nagrath, A. Messac, B. W. Bequette, S. M. Cramer

4:35 —142. Salt effects on protein RPC retention and conformation. E. J. Fernandez, J. M. Sokol, J. O'Connell

4:55 —143. Microcalorimetry study of the interactions between poly(N-isopropylacrylamide) microgels and amino acids. H. Bianco-Peled, O. Kimhi

 Section C

Advances in Vaccine Development, Gene Delivery, and Gene Therapy

2:00 — Introductory Remarks.

2:05 —144. Live, oral, attenuated Salmonella typhi vaccine strains as carriers of heterologous antigens. E. M. Barry, A. Santiago, M. M. Levine

2:25 —145. Toll-4 dependent adjuvant activity of emulsan. B. Panilaitis, L. Glickstein, J. Fuhrman, D. L. Kaplan

2:45 —146. Antibody responses to encapsulated group C meningococcal polysaccharide-tetanus toxoid conjugates. C. E. Frasch, C. Lee, J. Zhang

3:05 —147. Antigen-specific molecular sizing of a multivalent capsular polysaccharide vaccine by modern size-exclusion chromatography and nephelometry. Y. Yang, A. Matukonis, H. Hou, T. Kolachina, N. Kumar, P. McVerry, X. Wu

3:25 —148. Strategies of purification process development for bacterial recombinant vaccines. T. Yuan, Y. Yang

3:45 — Intermission.

4:05 —149. A nicotine conjugate vaccine for the possible treatment and prevention of nicotine addiction. A. Fattom, S. Ennifar, G. Horwith, S. Fuller, P. Pentel, D. Malin, R. Naso

4:25 —150. Increase capsular polysaccharide production using fed-batch. Y. He, S. Mindel, E. McGill, D. Anderson, T. Kim, S. Carter, M. Brancieri, A. Lages

4:45 —151. Enzymatic versus chemical pretreatment of the fermentation broth of N. meningitidis to improve harvest recovery. A. Lages, S. Ahuja, B. Baibak

5:05 — Concluding Remarks.

WEDNESDAY MORNING

 Section A

Validation of Biopharmaceuticals Processes

8:00 — Introductory Remarks.

8:05 —152. An approach to second sourcing of raw materials. S. Abraham, H. Bevan, A. Thomas, A. Westlake

8:30 —153. Development and qualification of a scale-down cell culture model for process validation. R. L. deVries, A. Gardner, R. Gerber, P. McAllister

8:55 —154. Bioburden monitoring and establishment of bioburden specifications for bioprocesses. C. Z. Chen, P. Yegneswaran

9:20 —155. Process validation approach for monitoring the performance of tangential flow filtration membrane devices. J. T. Petrone, T. Erdenberger, C. Esenther

9:45 — Intermission.

10:15 —156. Design of a new chromatography column for cleaning effectiveness. G. Kierans, C. Antoniou, H. Carter, T. Dennen, A. Noyes

10:40 —157. Impact of column hardware on CIP and resolution during the scale-up in preparative biochromatography. F. Drewe, J. B. Lee

 Section B

Advances in Cell Culture Process Development

8:00 — Introductory Remarks.

8:05 —158. Optimization of animal-component-free medium for CHO cell growth and recombinant protein production using statistical design. R. Bhatia, C. Robberson, T. Osborne, S. Walfish, J. Zhang

8:25 —159. Development of feed concentrates for CHO fed-batch processes. B. Horvath, S. Gu

8:45 —160. Enhancing batch efficiency by harvest/fill operations in manufacturing. S. Chen, A. Dadson, K. Carswell, F. Situ, A. Wajid

9:05 —161. A challenge with Vero cell attachment in large-scale nunc cell factories. I. Blumentals, C. Ranucci, S. Choudhari, A. Harris, E. Houghton, B. Mowrer, S. Ozuna, R. Scott, J. Whipple, G. Zang, J. Aunins, C. Goochee

9:25 —162. Protein glycosylation is controlled by cell culture conditions. M. Butler, N. Huzel, M. Yang, V. Restelli, J. Kunkel

9:45 — Intermission.

10:10 —163. Controlling product quality of a recombinant antibody produced in CHO cells by optimizing cell culture conditions. J. C. Goodrick, S. Kaneshiro, R. Kiss, T. Monica

10:30 —164. Bottleneck analysis of the glycosylation pathway for galactose-fed CHO cells. K. J. R. Clark, S. W. Harcum, K. M. Bailey, F. W. R. Chaplen

10:50 —165. Enhanced specific productivity at low culture temperature in recombinant Chinese hamster ovary cells depends on the integration site of a foreign gene. S. K. Yoon, G. M. Lee

 Section C

Advances in Metabolic Engineering

8:00 —166. Use of metabolic models in metabolic engineering. J. Forster, J. Nielsen

8:23 —167. Large-scale cybernetic modeling of protease production in Bacillus subtilis. J. Varner

8:46 —168. What can we learn from systems biology? J. S. Edwards

9:09 —169. Quantification and characterization of uncertainty in metabolic engineering. V. Hatzimanikatis, I. Birol, J. Shi, L. Wang

9:32 —170. Numerical innovations in modeling and simulation of carbon bond labeling experiments. G. Sriram, J. V. Shanks

9:55 — Intermission.

10:10 —171. Metabolic engineering of folic acid production. T. Zhu, R. Koepsel, M. M. Domach, M. Ataai

10:33 —172. Designing novel metabolic control in Escherichia coli. W. C. Wong, S. Lee, E. Fung, J. C. Liao

10:56 —173. Metabolic engineering of acetyl-CoA node and its effect on metabolic flux redistribution in chemostat Escherichia coli cultures. R. V. Vadali, G. N. Bennett, K. San

 Section D

2002 Elmer Gaden Award

11:40 —174. Scalable bioprocesses for the controlled differentiation of embryonic stem cells. P. W. Zandstra

WEDNESDAY AFTERNOON

 Section A

Regulatory Science: An FDA Perspective

1:45 — Introductory Remarks.

1:50 —175. Analytical methods development at FDA CBER: Analytical Chemistry staff, Office of Vaccines Research and Review. A. Del Grosso, J. May

2:10 —176. FDA CDER's perspective on specifications for peptide maps. S. K. Moore

2:30 —177. Product-directed research in the division of monoclonal antibodies. K. Brorson

2:50 —178. Regulatory considerations for rapid development of biological products. P. G. Swann

3:10 —179. Substrate specificity studies on recombinant Campylobacter jejuni N-acetylneuraminic acid synthetase. A. Sundaram, W. Vann

 Section B

Advances in Biocatalysis

2:00 —180. Pharmaceutical biocatalysis: Challenges of whole-cell bioconversions. D. J. Pollard, K. Telari, C. McWilliams, G. Humphrey, R. Greasham

2:25 —181. Ketoreductases in the synthesis of valuable chiral intermediates: Application in the synthesis of statine, phenylstatine, and their analogs. S. Kambourakis

2:50 —182. Cofactor regeneration of NAD⁺ from NADH: Novel water-forming NADH oxidases. B. R. Riebel, P. R. Gibbs, W. B. Wellborn, A. S. Bommarius

3:15 —183. Improving redox biocatalysis by engineered cells. J. D. Stewart, A. Z. Walton, I. Kaluzna, B. D. Feske

3:40 — Intermission.

4:00 —184. Practical biohydroxylation with Sphingomonas sp. HXN-200: A highly active, regio- and stereoselective, and easy-to-handle biocatalyst. Z. Li, B. Witholt

4:25 —185. Application of a Baeyer-Villiger monooxygenase from Cunninghamella echinulata NRRL 3655. C. S. Whitcher, J. A. Littlechild, R. Wohlgemuth, J. M. Woodley

4:50 —186. Oxygen limitations in the biocatalytic BVMO catalyzed synthesis of lacones. C. V. F. Baldwin, J. M. Woodley

 Section C

Advances in Metabolic Engineering

2:00 —187. Expression profiling and high-temperature pressure adaptation of the hyperthermophilic methanarchaeon, Methanococcus jannaschii. B. B. Boonyaratanakornkit, A. J. Simpson, C. B. Park, C. Fraser, N. M. El-Sayed, D. S. Clark

2:25 —188. Carbon flux analysis of metabolic reactions in cell-free protein synthesis. K. A. Calhoun, J. R. Swartz

2:50 —189. Metabolic engineering of yeast for polyketide biosynthesis. N. A. Da Silva, S. Lee, K. K. M. Lee, C. G. Ching, S. Mutka, J. T. Keasley

3:15 —190. Engineering Streptomyces bacteria to overproduce natural products. C. Kao, A. Lum, J. Huang, S. Cohen, C. R. Hutchinson

3:40 — Intermission.

4:00 —191. Rewiring cellular circuitry to improve biomanufacturing processes. E. M. Driggers, J. Royer, M. Askenazi, K. Madden, R. Doten, D. Zimmer, T. Feibelman, J. Trueheart, B. Cali

4:25 —192. Target hierarchy: The cases for interfering RNA and transient controllers. W. E. Bentley, S. F. Kramer, M. P. DeLisa, R. Srivastava, L. Wang, K. Carter, C. Tsao

4:50 —193. DNA array-based transcriptional analysis of the Clostridium acetobutylicum differentation and degeneration programs. E. T. Papoutsakis, C. Tomas, K. Alsaker, H. Bonarius, W. Hendriksen, J. Beamish, H. Yang, C. Paredes

5:15 —194. A functional protein chip for in vitro metabolic engineering. G. Stephanopoulos, G. Jung

 Section D

Advances in Tissue Engineering and Cell Therapy

2:00 — Introductory Remarks.

2:05 —195. Development of in vitro and in vivo models of wound healing based on engineered tissues: A novel role of fibrin in wound healing. D. J. Geer, D. Swartz, S. T. Andreadis

2:25 —196. Use micropatterned biodegradable materials to control cell behaviors. Y. Wang, C. Ho

2:45 —197. Organization of cell-adhesion proteins at surfaces on a subcellular-length scale using colloidal particle assembly. N. J. Gleason, J. D. Carbeck

3:05 —198. Mathematical model for the depth-filtration seeding of human mesenchymal stem cells in 3-D nonwoven PET matrices. W. L. Grayson, T. Ma

3:25 —199. Physical and mechanical properties of porous scaffolds fabricated from a blend of starch and chitosan. A. Subramanian, H. Lin, M. Hanna, K. Kim

3:45 — Intermission.

4:15 —200. Polysaccharide-based composite scaffolds for tissue engineering applications. A. Moshfeghian, Y. Huang, A. Sarasam, R. Lanman, S. Madihally

4:35 —201. Modeling of cell growth and nutrient consumption in hematopoiesis in a 3-D perfusion bioreactor. P. Pathi, T. Ma, B. R. Locke

4:55 —202. Tumor angiogenesis model with scaffold of small intestinal submucosa. H. Yang, R. R. Rivera, T. Ma

 Section E

The Process of Process Development

3:40 — Introductory Remarks.

3:45 —203. Structured decisional-support tool for rapid bioprocess modeling. A. C. Lim, S. Farid, J. Washbrook, N. J. Titchener-Hooker

4:10 —204. Principles of fermentation process development and characterization: Application to fermentation development for vaccine manufacture. T. Lee, J. H. Crinean, J. R. Olges-Murphy, B. D. Bahler, S. H. Bauer, R. L. Hopfer, J. L. Cowell

4:35 —205. Selection of chromatographic media and conditions for preferred recovery processes. T. N. Breece, T. M. Nguyen, C. Schmelzer

5:00 —206. Using a high-throughput screening system for the development of scalable protein crystallization processes. V. Klyushnichenko

5:25 —207. Enabling faster process development: Automation of process monitoring assays. P. DePhillips, A. Bowman, K. Little, M. Vaccaro, M. Wenger, A. Lee

5:50 — Concluding Remarks.

Processes for Utilization of Agricultural By-Products

WEDNESDAY EVENING

 Section A

Poster Session

6:00 - 9:00

208. Novel fluorescent pH indicator. G. G. Yi, Z. Diwu, L. Lavis, Y. Chen, J. Twu

209. Overcoming the barriers to batch process scheduling in the biopharmaceutical industry. D. P. Petrides, C. Siletti

210. Cloning of nattokinase genes and its preliminary studies. J. Ling, W. Du, L. Luo, R. Yang

211. Construction of a vector permitting immobilization and visualization of enzymes on compatible polymeric surfaces. E. Sahin, A. Taralp, Z. Sayers

212. Investigation of expression strength of various antibody gene arrangements in targeted CHO genome using an flpin CHO system. P. Fan, J. Larson, H. Qian, J. zhu

213. Medium characterization and optimization by amino acid screening and design of experiment. J. H. Crinean, J. R. Olges-Murphy, T. Lee, S. H. Bauer, R. L. Hopfer, J. L. Cowell

214. Utilizing microarray technology for rapid identification of influenza A. M. Townsend, K. Rowlen

215. Morphological changes and extent of autolysis in filamentous fungi as a response to pulse-feeding of nutrients. J. Kadarusman, M. R. Marten

216. Characterization and transcriptional analysis of three adjacent Thermotoga maritima genes that show significant similarity to both aminopeptidases and endoglucanases. J. K. Michel, D. E. Ward, S. B. Conners, R. M. Kelly

217. A tool for the rapid evaluation of process limitation using microscale experimentation. F. Ahmed, G. J. Lye, J. M. Woodley

218. Effects of bionanoconjugation on protein-DNA interactions in Lac Repressor. M. K. Calabretta, B. D. Yuhas, V. L. Colvin, K. S. Matthews

219. Using atomic force microscopy to study micromechanical properties of the model fungus Aspergillus nidulans. L. Zhao, M. R. Marten, D. Schaefer

220. Using proteomics to understand how rapidly changing nutrient environments can affect E. coli during fed-batch fermentation. B. Raman, M. R. Marten, M. P. Nandakumar

221. A simple process strategy in filamentous fungal fermentation can significantly improve recombinant protein productivity. S. Bhargava, M. R. Marten, K. S. Wenger

222. Engineering of a de novo disulfide bond formation pathway in E. coli. L. Masip, J. Collet, M. P. DeLisa, J. C. A. Bardwell, G. Georgiou

223. Strategic engineering of R-oxynitrilase from almonds for industrial application. A. Glieder, W. Skranc, P. Poechlauer, H. Schwab, K. Gruber, R. Weis, M. Wubbolts, H. Mayrhofer

224. The cDNA-derived amino acid sequence of hemoglobin II from Lucina pectinata. J. López-Garriga, C. L. Cadilla-Vázquez, E. Torres-Mercado

225. Tranfer of nisin gene cluster into the chromosome of B. subtilis 168. J. N. Hansen, S. Yüksel

226. Transchip: A new system for studies of in vitro transcription of whole genomes. T. Wu, H. Yu, C. Churas, S. Zhou, D. C. Schwartz

227. Generation of recombinant influenza virus using baculovirus as a delivery vehicle. R. Grabherr, K. Poomputsa, C. Kittel, L. Toellner, W. Ernst, A. Egorov

228. Increasing acetyl-CoA pool in the presence of overexpressed phosphoenolpyruvate carboxylase or pyruvate carboxylase enhances succinate production in Escherichia coli. H. Lin, R. V. Vadali, G. N. Bennett, K. San

229. Enhanced isoamyl acetate production in Escherichia coli upon manipulation of the acetyl-CoA node. R. V. Vadali, K. San, G. N. Bennett

230. Expression studies of affinity-tailed green fluorescent proteins in Eschericia coli: Batch productivity differences when different immobilized metal affinity chromatography tails are employed. H. Liu, R. R. Beitle

231. Expression and secretion of active lignin peroxidase in the yeast Kluyveromyces lactis. N. A. Da Silva, D. Shah, W. Panuwatsuk

232. An investigation into the size-activity relationship of RNA catalysis. T. M. Coleman, F. Huang, J. Manning

233. Protein engineering for shifting pH optimum of Bacillus circulans xylanase. J. Yang, Y. S. Choi, Y. J. Yoo

234. Interaction of partially folded BPTI analogs with C191/C220A trypsin. I. V. Getun, J. Tulla-Puche, C. Woodward, G. Barany

235. Cofactor metabolic engineering: Comparative study of the effect of increasing NADH availability in E. coli chemostat cultures on minimal and complex medium. A. M. Sanchez, K. San, G. N. Bennett

236. Semiparametric regression analysis on yeast gene regulation prediction. F. Pan, X. Hu, W. Perrizo

237. Probing aromatic amino acid biosynthesis in Archaeoglobus fulgidus using DNA microarray. L. Rohlin, S. Lim, I. Schroeder, H. Monbouquette, J. C. Liao

238. Diagnosing biopharmaceutical fermentations using transcriptional profiling. J. M. Bonomo, R. T. Gill

239. Metabolic engineering of Saccharomyces cerevisiae for the production of isoprenoids. E. M. Paradise, G. Y. Wang, J. D. Keasling

240. Directed evolution to determine structure-function relationships in oxidosqualene cyclases. S. P. T. Matsuda, M. J. R. Segura, S. Lodeiro

241. Metabolic engineering of yeast for the production of 1,2-propanediol. N. A. Da Silva, W. Lee

242. Metabolomic application of NMR and MS: Tools for broad-screening metabolic pathways. T. W. Fan, R. M. Higashi, A. N. Lane

243. Metabolic modeling for evaluating effects of pressure pulsation on glycerol production by Candida krusei. W. Huang, G. T. Tsao

244. Monitoring cell culture by LDH measurements. J. C. Lagos, Y. Tsao

245. Experimental versus simulated calibration mdels for in situ FTIR monitoring of bioprocesses. S. Valentinotti, H. Kornmann, M. H. Rhiel, U. von Stockar

246. Bioreactor optimization for the production of a secreted reporter protein in CHO cells using an inducible expression system. D. S. Kompala, M. L. Lipscomb, M. C. Mowry

247. Feeding strategy optimization for mammalian cell culture. S. F. Gorfien, W. C. Paul, J. L. Walowitz, D. A. Judd, L. D. Tescione, S. J. Jacobia, D. W. Jayme

248. A multidimensional Kla model to expedite cell culture process transfer. J. A. Groves, C. Chun, T. Ryll, S. Chamow

249. Adaptation and propagation of A549 cells in suspension culture for the production of an adenoviral vector. Z. Liu, R. Longley, L. Radzniak, M. Santoro, M. Voloch

250. Analytical methods for mammalian cell culture feed streams. D. K. Rice II, J. Zdanowicz, J. S. Goldfuss

251. Applications of red fluorescent protein markers for indicating therapeutic protein expression and pericellular oxygen concentrations. C. W. Yung, Y. Hashimoto, W. E. Bentley, T. A. Barbari

252. Investigation of bivalent antibodies binding on fluid supported phospholipid membranes: The effect of hapten density. T. Yang, O. K. Baryshnikova, H. Mao, M. A. Holden, P. S. Cremer

253. A novel heat-shock protein prevents amyloid formation and toxicity. S. Lee, T. Good, K. Carson, A. Rice Ficht

254. Control of temperature and pH enhances human monoclonal antibody production in CHO cell culture. S. Oguchi, H. Saito, M. Tsukahara, H. Tsumura

255. Expression of EGFP reporter protein with a recombinant vaccinia virus: Comparison of microcarrier and cell-suspension-based bioreactor systems. N. A. Bleckwenn, W. E. Bentley, J. Shiloach

256. Influence of mixing on phenotypic differences in well and poorly differentiated prostate cancer spheroids. H. Song, K. O'Connor, C. Giordano, S. Clejan, O. David, H. LeBeau

257. A mini-bioreactor system for accelerated process development. R. Chen, S. Rajendran, K. Haverty, M. Tomkalski, P. Teplitsky, G. Wei, K. Namdev, R. Chillakuru

258. pH-Sensitive surfactants for water-in-oil xanthan fermentation. A. S. Restrepo, L. Ju, J. M. Bell, J. Y. Xu

259. Conversion of unsaturated fatty acids by compost bacteria. T. M. Kuo

260. High-throughput screening of heterologous P450 expression in yeast. J. A. Morgan, H. Jiang, D. chen

261. Microbubble fermentation of recombinant Pichia pastoris for human serum albumin production. W. Zhang, F. A. Agblevor

262. Methods for evaluation of composite latex coatings containing Gluconobacter oxydans: Accurate determination of the effectiveness factor for the oxidation of D-sorbitol to L-sorbose in oxygenated microbioreactors. C. W. Solheid, S. P. Charaniya, U. Diel, M. C. Flickinger

263. Control of methanol feed for Bacillus methanolicus fermentation via mass spectrometry. C. F. Komives, C. Wang, S. Rech

264. Biopesticide production through fermentation and entrapment of metarhizium anisopliae spores. Y. chen, J. Vaughan, F. A. Agblevor

265. Biosynthesis of germanium oxide by marine diatoms. G. L. Rorrer, C. Chang, C. Jeffryes

266. Continuous pH monitoring during shake flask fermentations using optical sensors. H. R. Kermis, Y. Kostov, P. Harms, G. Rao

267. On-line process monitoring of moisture measurements and reaction kinetics by near-infrared spectroscopy for a polysaccharide vaccine. J. B. Fletcher, E. P. Wen, S. L. Sagar, A. L. Lee

268. On-line cell mass monitoring of Saccharomyces cerevisiae fermentations by multi-wavelength fluorescence. M. Haack, A. Eliasson, L. Olsson

269. Large-scale expression and purification of hemoglobin I from Lucina pectinata. E. A. Collazo-Santiago, J. Lopez-Garriga, C. L. Cadilla

270. Characterization of fermentation process by design of experimental to reduce project time. J. R. Olges-Murphy, J. H. Crinean, T. Lee, S. H. Bauer, R. L. Hopfer, J. L. Cowell

271. The role of computer modeling in bioprocess development and technology transfer. C. Siletti, D. Petrides

272. Design of experiment applied to fermentation process optimization and characterization for validation. B. D. Bahler, T. Lee, S. H. Bauer, R. L. Hopfer, J. L. Cowell

273. Technical and economic feasibility of a novel, low-energy sterilization process. J. Zhang, J. Li, H. Kaiser, G. L. Thomson, T. Thomas, T. Davis, M. Matthews

274. Sample self-displacement purification of an antisense oligonucleotide by anion exchange membrane chromatography. A. Lajmi, T. Warner, Y. Sanghvi

275. Compaction agent clarification of microbial lysates. B. DeWalt, J. C. Murphy, T. Cano, J. Zijffer, G. E. Fox, R. C. Willson

276. Membranes from whey protein isolate: Studies of flux and rejection. J. Y. Teo, R. R. Beitle

277. Alternatives to protein A resin evaluated for the purification of human antibodies. R. Alsop, L. Nguyen, G. Zapata

278. Dissolved oxygen affects the accumulation of recombinant clostridia peptide fragment in E. coli. N. G. Dalal, J. Geva, R. Fass, J. Shiloach

279. Aromatic thiols as redox buffers: Increasing folding rates for disulfide-containing proteins. J. D. Gough, W. J. Lees

280. Characterization and optimization of a pseudobioaffinity-based antibody separation method. A. Subramanian, J. Hommerding, S. Roy, C. Mascoli

281. Hydrophobic interaction chromatography: Effect of water, salt types, and process modeling. F. Xia, D. Nagrath, S. M. Cramer

282. Chromatography resin evaluation procedures. M. E. Cameron, R. Seely, K. Sabados, H. Harker

283. Development of a library of cation-exchanger salt-tolerant media based on a multimodal interaction approach. J. Maloisel, N. Thevenin, E. Brekkan

284. Purification of 2-gram parenteral, low copy-number plasmid for phase I/II clinical trials on an ÄKTA purifier chromatography system. S. Herzer, Z. Mei, C. L. DeSombre, X. Zhang, D. R. Rill

285. Scale-down of continuous filtration for rapid bioprocess design. T. S. Reynolds, R. M. Boychyn, M. Bulmer, J. More, M. Hoare

286. Evaluation of filters for the removal of E. coli proteins. C. W. Richey Jr., J. Seely, H. Rushing

287. Streamlining Escherichia coli S30 extract preparation for economical cell-free protein synthesis. D. V. Liu, J. F. Zawada, J. R. Swartz

288. The evaluation of column performance over the lifetime of sepharose high-performance resin. D. J. Dripps, T. Taggart, T. Kessler, M. Cameron, R. Todd, J. Seely

289. Topoisomerase-targeting anticancer drug screening by capillary electrophoresis. J. Chen, G. Liu, M. Du, Y. Huang, Y. Ma

290. A new pectate lyase from Bacillus pumilus for bioscouring. B. Klug, W. Schnitzhofer, K. Robra, G. M. Guebitz

291. Differential stability of a monoclonal antibody in acetate, succinate, citrate, and histidine buffer systems. N. Piros, M. E. M. Cromwell, S. Bishop

292. Effect of stainless steel exposure on the stability of a recombinant humanized IgG1 monoclonal antibody. R. Wong, S. Lo, C. Quan, W. Lau, S. Wang

293. Chip devices for CEC of peptides and proteins using various stationary phase materials. R. Jindal, S. M. Cramer

294. Evaluating chromatographic process variability using design of experiments. E. R. Cunningham, D. Myers, C. Bork, L. E. Conley

295. Highly concentrated formulations of the HIV-1 entry inhibitor PRO 542. K. Prakash, L. Zhao, D. Fisch, S. Rosenfield, K. Nagashima, N. Schülke, W. Olson

296. Bridged diarylmethane fluorescent probes for labeling biomolecules. W. Zhou, Z. An, J. Lavin, S. R. Marder

297. Hydrophobic interaction chromatography: Using design of experiments to determine critical process parameters. M. W. Baillargeon, D. A. Ross, D. Eisenhauer, E. O. Lundell

298. Enzymatic method for detection of specific DNA-protein interactions. G. E. Sroga, J. S. Dordick

299. Preparative continuous annular chromatography: Investigations on the "peak wobbling" phenomenon. R. van Wegen, J. Prior

300. Screening of isolated cells via arrays of impedance sensors. D. Nguyen, H. Xiaoqiu, T. Afentakis, M. Hatalis, M. Domach, D. Greve

301. Characterization of 3-D collagen hydrogels for functional cell-based biosensing. C. Mao, W. S. Kisaalita

302. A novel immunoassay for atrazine using tunable immunosorbent. J. Kim, A. Mulchandani, W. Chen

303. Establishment of efficient systems to quantify human monoclonal antibody for the selection of production cells. T. Nakagawa, Y. Takamatsu, M. Tsukahara, T. Kadoya, H. Tsumura

304. Objective and quantitative method for titration of nonoccluded baculovirus. O. T. Ramírez, J. A. Mena, L. A. Palomares

305. New tissue micro-osmometer. F. Horkay, A. S. Berman, P. J. Basser

306. Direct cell and tissue analysis by infrared DIOS. S. M. Dutta, D. J. Rousell, K. K. Murray

307. Measurement of the elastic modulus of thin gel layers by the atomic force microscope. E. K. Dimitriadis, F. Horkay, B. Kachar, R. S. Chadwick

308. Microfluidic chip detection using MALDI mass spectrometry. D. A. Narcisse, H. K. Musyimi, X. Zhang, S. A. Soper, K. K. Murray

309. Novel assembly platform for integrating biological species within MEMS devices. H. Yi, L. Wu, D. A. Small, T. Chen, R. Ghodssi, G. W. Rubloff, G. F. Payne, W. E. Bentley

310. Protein aggregation kinetics: Applications to protein A chromatography. P. Gupta, A. A. Shukla

311. Bti toxin Cyt1A in the lipid bilayer: Aggregation and the membrane damage. S. D. Manceva, P. S. Russo, M. Pusztai-Carey, P. Butko

312. Biodegradable microparticles as potent delivery systems for vaccines: Experimental measurement of factors governing antigen adsorption and efficacy. J. Chesko, J. Kazzaz, M. Ugozzoli, M. Singh, D. O'Hagan

313. Stable surface attachment and detection of fusion proteins. S. B. Brueggemeier, S. P. Palecek

314. Use of biarsenic labels to monitor protein-protein interactions. H. Eliason, M. S. Shekhani, M. Robers, K. W. Vogel, G. T. Hanson, K. L. Vedvik, C. Rüttimann-Johnson, N. Qadir

315. Automated docking of phospholipids to the phospholipase D active site: Insight into the catalytic mechanism. C. L. Aikens, A. Laederach, P. J. Reilly

316. Effect of molecular weight on the expression and characterization of a self-assembling peptide. W. D. Marner II, N. L. Goeden-Wood, J. D. Keasling, S. J. Muller

317. Synthesis of protein-based artificial transaminase by site-directed mutagenesis and chemical modification. C. Tann, D. Qi, M. D. Distefano

318. Comparison of immunoassay-blocking strategies on metal oxide substrates. A. N. Scribner, C. L. Cole, R. J. Colton, L. J. Whitman

319. Synthesis and biophysical characterization of template-assembled GALA. D. H. Haas, R. M. Murphy

320. Evolvability of random polypeptides through functional selection. A. Yamauchi, I. Urabe, T. Yomo

321. Generation of a semisynthetic oxidoreductase via chemical and genetical modication. C. Tann, M. D. Distefano

322. Elimination of oxygen limitation in enzmetic reactions. A. T. Y. Cheng

323. Hydrolysis of oligosaccharides using strong cation exchange catalyst and cellulase enzymes. Y. M. Kim, N. S. Mosier, R. Hendrickson, J. Goetz, M. R. Ladisch

324. Activity and kinetics of bovine pancreatic ribonuclease in organic-aqueous media. S. Emadi, F. Masdar-al Omour

325. Effect of silicon atom in substrates on (R)-oxynitrilase-catalyzed enantioselective synthesis of (R)-ketone cyanohydrin. N. Li, M. H. Zong, C. Liu, H. S. Peng

326. Enzymatic ammonolysis of trimethylsilylmethyl acetate in microaqueous phase. H. S. Peng, M. H. Zong, Y. Q. Xu

327. Enzymatic enantioselective synthesis of (R)-2-trimethylsilyl-2-hydroxyl ethylcyanide. N. Li, M. H. Zong, C. Liu, H. S. Peng

328. Lipase-catalyzed enantioselective esterification of 1-(trimethylsilyl)ethanol in organic solvent. H. Wu, M. H. Zong, D. H. Luo

329. Effects of various parameters on the enzymatic degradation of chlorogenic acid in model and sunflower meal systems. E. R. Martinez, Z. Duvnjak

330. Enzyme mediated production of free lutein from marygold flowers in nonaqueous media. E. Bárzana, M. J. Mora-Pale, S. Pérez-Munguía, M. López-Vargas, A. Lopez-Munguia

331. Production of biodiesel fuel from soybean oil catalyzed by lipozyme TL in a solvent-free medium. Y. Xu, W. Du, D. Liu

332. Structural differences between bovine and porcine vitreous humor. F. Y. Ohene

333. Studies on coagulation factor VIIa autoproteolysis and formation of degradation products. J. C. Krarup, T. B. Hansen

334. Collagen-hydroxyapatite multilayer sheet (fish scale model). K. Yamauchi, T. Tanabe, T. Goda

335. Controlled nanostructures assembly based on DNA-protein interactions. G. E. Sroga, J. S. Dordick

336. In vivo gene transfection into rat brain cells: A comparison between viral, polyethyleneimine, and calcium phosphate nanoparticle methods. T. D. Corso, I. Roy, M. Bland, T. Buckley, A. S. Gambino, L. C. Jenkins, B. Cranston, J. Myers, E. Stachowiak, E. J. Bergey, P. N. Prasad, D. Bloom, W. J. Bowers, M. K. Stachowiak

337. Surface characterization and in vitro protein adsorption studies of sol-gel derived ultrathin titanium oxide layers. M. C. Advincula, X. Fan, J. E. Lemons, R. Advincula

338. Binding of perfluorooctanoic acid to rat and human plasma proteins. X. Han, T. A. Snow, R. A. Kemper, G. W. Jepson

THURSDAY MORNING

 Section A

Advances in Biocatalysis

8:00 — Introductory Remarks.

8:05 —339. Accelerated biocatalyst stability testing for process optimization. P. R. Gibbs, U. Neunert, A. S. Bommarius

8:25 —340. Multi-enzymatic synthesis of xylulose 5-phosphate. J. Shaeri, E. B. Rathbone, I. Wright, R. Wohlgemuth, J. M. Woodley

8:45 —341. Natural vanillin synthesis from corn bran. R. R. Chen, N. Win, K. Brunson

9:05 —342. Formation of lactobionic acid by a novel biocatalytic process. R. Ludwig, M. Ozga, M. Zamocky, K. D. Kulbe, D. Haltrich

9:25 —343. Biocompatible and degradable biocatalytic dextran hydrogels. L. S. Ferreira, A. M. S. Cabrita, A. Rafael, M. H. Gil, J. S. Dordick

9:45 — Intermission.

10:15 —344. Surface hydrophobicity of silica supports effects activity of immobilized enzymes in organic reactions. J. P. Lindsay, R. F. Wormsbecher, J. S. Dordick

10:35 —345. Optimizing the cell concentration in thin, microporous latex biocatalytic coatings containing nongrowing Gluconobacter oxydans for very high oxidation rates of D-sorbitol to L-sorbose. S. P. Charaniya, C. W. Solheid, M. C. Flickinger

10:55 —346. Efficient encapsulation of alcohol dehydrogenase in silicate matrix via sol-gel process as biocatalyst. Z. Jiang Sr.

 Section B

Continuous Bioprocessing

8:00 — Introductory Remarks.

8:05 —347. Continuously perfused fluidized bed fermentation for large-scale production of r-protein and monoclonal antibodies: Process stability and scale-up. G. Blueml, K. Landauer, M. Duerrschmid, S. Wiederkum, H. Klug, O. Doblhoff-Dier, H. Katinger

8:25 —348. Quantifying physiology and metabolism of mammalian cells in continuous perfusion culture. C. Goudar, R. Heidemann, J. H. Vogel, J. Michaels, K. B. Konstantinov

8:45 —349. Protein refolding by continuous chromatography. H. Lanckriet, A. P. J. Middelberg

9:05 —350. Continuous matrix-assisted refolding using annular chromatography: A technology for quantitative conversion of unfolded proteins into native proteins. A. Jungbauer, C. Machold, R. Schlegl, W. Buchinger

9:25 —351. Kinetics and reactor design for continuous and semicontinuous processing of cellulosic biomass via simultaneous saccharification and fermentation. X. Shao, C. E. Wyman, L. R. Lynd

9:45 — Intermission.

10:10 —352. Solvent gradient operation of simulated moving bed processes. M. Mazzotti, S. Abel, L. Aumann, M. Morbidelli

10:30 —353. In-line, online optimization of a new SMB technology working as single-column system. P. Poetschacher

10:50 —354. Advances toward process-scale countercurrent chromatography and the prospect of continuous countercurrent extraction. I. Sutherland

 Section C

Mechanistic Aspects of Virus-Cell Interactions

8:00 — Introductory Remarks.

8:05 —355. Simulations of virus binding using Brownian adhesive dynamics. D. A. Hammer, T. English

8:25 —356. Cell targeting using metabolically biotinylated adenoviral vectors. M. A. Barry, M. B. Parrott, S. Campos, K. E. Adams

8:45 —357. Study of the adsorption effect on the diffusion of recombinant adenoviruses toward 293S cells. A. Garnier, A. Gilbert, A. Kamen

9:05 —358. A controllable gene delivery muscle patch. J. Bonadio, M. Salva

9:25 —359. Monitoring viral spread and the development of cellular defensive responses to vesicular stomatitis virus infections in vitro. V. Lam, K. Duca, J. Yin

9:45 — Intermission.

10:15 —360. Retrovirus gene transfer to epidermal stem cells: The role of integrins and extracellular matrix. S. T. Andreadis, B. Bajaj

10:35 —361. Effects of lipid rafts on the dynamics of retrovirus trafficking. K. Lim, J. Yin

10:55 —362. Designing a gene therapy to prevent AIDS but maintain HIV. L. S. Weinberger, D. V. Schaffer, A. P. Arkin

Processes for Utilization of Agricultural By-Products

THURSDAY AFTERNOON

 Section A

Advances in Biocatalysis

2:00 —363. Directed evolution of alkane hydroxylation activity in cytochrome P450 BM-3. F. H. Arnold, M. W. Peters, P. Meinhold, E. T. Farinas, A. Glieder, C. W. Bugg

2:20 —364. Engineering a protein molecular switch by combinatorial domain insertion. M. Ostermeier, G. Guntas

2:40 —365. Enzymology of xylose utilization in yeast: Coenzyme specificity of xylose reductase from Candida tenuis analyzed by site-directed mutagenesis and X-ray crystallography. B. Nidetzky, B. Petschacher, M. Klimacek, K. L. Kavanagh, D. K. Wilson

3:00 —366. Novel screening method for enzyme activity and enantioselectivity using surface-enhanced resonance Raman scattering. L. C. Stevenson, D. Graham, B. D. Moore

3:20 —367. Flow cytometric screening of chimeric glutathione transferase libraries expressed in Escherichia coli. K. E. Griswold, Y. Kawarasaki, T. Selzer, J. D. Stevenson, S. Benkovic, B. L. Iverson, G. Georgiou

3:40 — Intermission.

4:10 —368. Microarray-based enzymatic modification of immobilized substrates for drug discovery. L. Santhanam, M. G. Hogg, J. A. Holland, J. S. Dordick

4:30 —369. Solid-phase biocatalytic amplification of the natural product bergenin. U. Akbar, J. S. Dordick, D. S. Clark

4:50 —370. Comparative thermostability/thermoactivity analysis of class II xylose isomerases for high-fructose corn syrup production at elevated temperatures. K. L. Epting, T. V. Pearce Jr., C. Vieille, D. Sriprapundh, J. G. Zeikus, R. M. Kelly

 Section B

Separations and Process Technology for Genetic Therapy and Diagnosis

2:00 — Introductory Remarks.

2:05 —371. The impact of nanotechnology on the development of cell isolation, separation, and expansion. G. Keller, J. Martinec, D. Richard

2:25 —372. Sequence-specific oligonucleotide purification using peptide surfactants. J. W. Schneider, J. P. Vernille, B. F. Marques

2:45 —373. Plasmid purification by environmentally triggered precipitation. J. Kostal, A. Mulchandani, W. Chen

3:05 —374. Plasmid DNA production using compaction agents and immobilized metal-affinity chromatography. T. Cano, J. Murphy, G. E. Fox, R. C. Willson

3:25 —375. Purification of supercoiled plasmid DNA by selective calcium silicate adsorption of closely related impurities. M. A. Winters, J. D. Richter, S. L. Sagar, R. J. Lander

3:45 — Intermission.

4:05 —376. Metabolic biotinylation of proteins and viral vectors for purification from mammalian cells. M. A. Barry, M. B. Parrott, K. E. Adams, S. Campos, D. Ghosh

4:25 —377. Viral vectors for gene therapy: New purification challenges. S. Herzer, R. Morenweiser, N. Brument, P. M. Moore

4:45 —378. Review of novel technologies for use as a virus barrier to eliminate adventitious contamination of viral drug products. R. Pearce, H. Lutz

 Section C

Advances in Plant Biotechnology

2:00 — Introductory Remarks.

2:05 —379. Expanded- and fixed-bed adsorption in capture of proteins from plant extracts. C. E. Glatz, T. J. Menkhaus, Y. Bai

2:25 —380. Metabolic flux analysis of Catharanthus roseus hairy roots using a carbon bond labeling experiment. G. Sriram, B. Fulton, J. V. Shanks

2:45 —381. Metabolic flux analysis of halogenated monoterpene biosynthesis in microplantlet suspension cultures of marine red macroalgae. G. L. Rorrer, J. P. Polzin

3:05 —382. Study of polyunsaturated fatty acid synthesis in Glossomastix chrysoplastos, a marine microalgae, through optimization of bioreactor conditions and desaturase expression. T. Y. Hsiao, H. W. Blanch

3:25 —383. Investigating cell subpopulation dynamics of Taxus cell suspension cultures: A single cell method. M. C. Naill, S. C. Roberts

3:45 — Intermission.

4:05 —384. Metabolic engineering of indole alkaloid pathways in Catharanthus roseus hairy roots. E. H. Hughes, S. Hong, S. Gibson, J. V. Shanks, K. San

4:25 —385. Analysis of theoretical metabolic yields under photo-autotrophic conditions. J. A. Morgan, O. M. Akpobasah

Processes for Utilization of Agricultural By-Products