|
Instrument
|
Year |
Ionization method
and mass analyzer type |
Applications
|
| Bruker AUTOFLEX | 2002 | MALDI
Time of flight |
Characterization of proteins, oligonucleotides and other polymers. Characterization and identification of proteins by peptide mass mapping and sequencing. |
| Bruker ESQUIRE 3000 | 2002 | ESI, APCI
Ion Trap |
Analysis of non-volatile and thermally-labile compounds; protein mass determination and digest analysis; peptide sequencing. |
| Shimadzu GC-17A/QP-5000 | 1993 | +EI
Quadrupole |
Analysis of volatile (GC amendable) compounds |
| JEOL HX110/HX100 | 1986 | EI, CI, FD, FAB, ESI
Sector |
Accurate mass measurement |
| Kratos Kompact III | 1993 | MALDI
Time of flight |
Training |
| Manufacturer: | Bruker Daltonics, Billerica, MA, USA |
| Mass Range: | Linear: > 250 kDa Reflectron: > 5,000 Da |
| Resolution | Linear: > 4,000 (FWHM)
Reflectron: >10,000 (FWHM) |
| Ionization | Matrix Assisted Laser Desorption-Ionization (MALDI) |
| Applications | Characterization of proteins, oligonucleotides and other polymers. Characterization and identification of proteins by peptide mass mapping. Peptide sequencing by postsource decay (PSD). |
This instrument may be accessed by the researchers upon completion of a training session followed by several supervised runs. Because the instrument has been designed with the end user in mind its software is fairly easy to use. There are also few preset method files defining parameters for a wide range of experiments. A condensed version of operation instructions is available for download.
| Manufacturer: | Bruker Daltonics, Billerica, MA, USA |
| Mass Range: | 50-3000 |
| Resolution | Nominal mass measurement capability throughout the entire m/z range |
| Ionization | Electrospray ionization (ESI), atmospheric pressure chemical ionization (APCI) |
| Applications | Characterization of proteins, oligonucleotides. Characterization and identification of other ESI and APCI amendable compounds. |
| Manufacturer: | Shimadzu Scientific Instruments , Columbia, MD, USA |
| Mass Range: | 10-700 |
| Resolution | Nominal mass measurement capability throughout the entire m/z range |
| Ionization | Electron Impact (EI) in positive ion mode |
| Applications | Characterization and identification of GC amendable compounds. |
The GCMS-QP5000 compact, quadrupole mass spectrometer combines good performance with easy operation. Even first-time users can easily perform an analysis, evaluate the results, and print a report.
| Manufacturer: | JEOL, Tokyo, Japan |
| Mass Range: | 1- >10,000 |
| Resolution | Up to 100,000 |
| Ionization | EI, CI, FAB, FD, ESI |
| Applications | Mostly used for exact mass measurement using FAB (Fast Atom Bombardment) |
This instrument is one of the first MALDI-TOF machines made commercially available by Kratos (Manchester, UK). It is mostly used for undergraduate instruction.
