Seminar† -† March 24th,2008


Nonequilibrium theory1

Dispersion phenomena in chromatography


In the last decades, the importance of chromatography in purification and isolation of biological macromolecules have forced this arena to be revolutionized. Several studies have been made to develop better columns, trying to satisfy various contradictory requirements simultaneously: a high rate of efficiency, a reasonable analysis time, an acceptable inlet pressure and a low detection limit. To achieve those requirements, it is mandatory to minimize the dispersion phenomena that occur on the process. The nonequilibrium theory (theoretical approach, extensions, limitations and assumptions) is the tool employed to allow a better understanding of the dynamics of chromatography.

Finally, the dispersion phenomena and the packing processes cannot be studied apart, because the columnís bed structure plays an important role in both fields. Some efforts have been made earlier; however, one lacuna still exists in studding the dynamics of packing and dispersion phenomena, which is the aim of this study.







Nuno Pinto




1 Giddings, J.C., Dynamics of Chromatography, Marcel Dekker, Inc., New York (1965)