Nirav Shelat, Biochemistry
"Structural Development of Unknown Streptococcus Pneumonia Polysaccharide
through NMR Spectroscopy Analysis"
Faculty Mentor: Dr. C. Allen Bush
Expected Graduation Date: Spring 2010
The polysaccharide called “10F” is molecule attached to the cell surface of the bacteria Streptococcus Pneumoniae. This polysaccharide is one of the many serotypes for the Strep. Pneumoniae strain, and has a proposed structure that was published in 1979. However, research upon the polysaccharide’s gene cluster suggests that this published structure maybe quite different from the actual structure. In order to confirm these new findings, two-dimensional H1 and C13 NMR spectroscopy proves to be an extremely useful tool. By extracting data through the employment of various NMR spectra such as COSY, TOCSY, NOESY and HMBC, we will determine a definite molecular structure of this polysaccharide. Ultimately, our findings may help in the creation of new vaccines or treatments against the Streptococcus Pneumonia strain.